All Phenotypes Gt(ROSA)26Sor<tm1(HA)Libl> MGI Mouse

weight loss ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells, mice exhibit weight loss

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells, severely affected animals display difficulty to right themselves when overturned

tremors ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells (Tc1 cells), severely affected animals exhibit tremors

decreased locomotor activity ( J:137838 )

• 4 weeks after receiving transfer of HA-specific effector CD8 T cells, severely affected animals show reduced mobility

brain inflammation ( J:137838 )

• inflammation is also observed frequently in cerebellum, fornix and periventricular areas

spinal cord inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show spinal cord inflammation

• T cell infiltration into spinal cord starts on day 5 after transfer and peaks at day 28

optic nerve inflammation ( J:137838 )

• by 5 days following transfer of Tc1 cells, all mutants examined show optic nerve inflammation

abnormal oligodendrocyte morphology ( J:137838 )

• apoptosis in optic nerve and spinal cord is seen at early time points following Tc1 transfer

abnormal optic nerve morphology ( J:137838 )

• focal areas with reduced myelin density and thin myelin sheaths are observed in animals after transfer of Tc1 cells

• vacuolization is seen at day 5 after Tc1 cell transfer

demyelination ( J:137838 )

• by 5 days following transfer of Tc1 cells, demyelination is observed in the spinal cord and optic nerve

• from 9 days post-transfer, most mutants exhibit extensive demyelination in optic nerves

• by day 19, actively demyelinating lesions show severe axonal damage