Phenotypes associated with this allele

• Allele Symbol: E2f3^tm2.1Gle
• Allele Name: targeted mutation 2.1, Gustavo Leone
• Allele ID: MGI:3806814
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	E2f3^tm2.1Gle/E2f3^tm2.1Gle	FVB.129S6-E2f3^tm2.1Gle	MGI:3806835
hm2	E2f3^tm2.1Gle/E2f3^tm2.1Gle	involves: 129S6/SvEvTac * FVB/N * NIH Black Swiss	MGI:3806841
cx3	E2f1^tm1Meg/E2f1^tm1Meg E2f2^tm1Zubi/E2f2^tm1Zubi E2f3^tm2.1Gle/E2f3^tm2.1Gle	involves: 129 * FVB/N * NIH Black Swiss	MGI:3806839
cx4	E2f2^tm1Zubi/E2f2^tm1Zubi E2f3^tm2.1Gle/E2f3^tm2.1Gle	involves: 129S1/Sv * 129S6/SvEvTac * FVB/N * NIH Black Swiss	MGI:3806843
cx5	E2f1^tm1Meg/E2f1^tm1Meg E2f3^tm2.1Gle/E2f3^tm2.1Gle	involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss	MGI:3806844
cx6	E2f3^tm2.1Gle/E2f3^tm2.1Gle E2f7^tm1.1Gle/E2f7^tm1.1Gle E2f8^tm1.1Gle/E2f8^tm1.1Gle	involves: 129S6/SvEvTac	MGI:5473265

Genotype
MGI:3806835

hm1

• Allelic Composition: E2f3^tm2.1Gle/E2f3^tm2.1Gle
• Genetic Background: FVB.129S6-E2f3^tm2.1Gle

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:138289 )

N • unlike mice lacking both isoforms, in mice lacking only the E2f3a isoform no significant embryonic lethality is seen

Genotype
MGI:3806841

hm2

• Allelic Composition: E2f3^tm2.1Gle/E2f3^tm2.1Gle
• Genetic Background: involves: 129S6/SvEvTac * FVB/N * NIH Black Swiss

adipose tissue

decreased white adipose tissue amount ( J:138289 )

• in older mice compared to age-matched wild-type mice

Genotype
MGI:3806839

cx3

• Allelic Composition: E2f1^tm1Meg/E2f1^tm1Meg; E2f2^tm1Zubi/E2f2^tm1Zubi; E2f3^tm2.1Gle/E2f3^tm2.1Gle
• Genetic Background: involves: 129 * FVB/N * NIH Black Swiss

mortality/aging

perinatal lethality ( J:138289 )

cellular

decreased cell proliferation ( J:138289 )

• in MEFs after 5 and 6 days in culture compared to littermate-derived controls

Genotype
MGI:3806843

cx4

• Allelic Composition: E2f2^tm1Zubi/E2f2^tm1Zubi; E2f3^tm2.1Gle/E2f3^tm2.1Gle
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * FVB/N * NIH Black Swiss

normal phenotype

no abnormal phenotype detected ( J:138289 )

• born at the expected frequency, normal birth weights and mature without obvious defects

Genotype
MGI:3806844

cx5

• Allelic Composition: E2f1^tm1Meg/E2f1^tm1Meg; E2f3^tm2.1Gle/E2f3^tm2.1Gle
• Genetic Background: involves: 129S4/SvJae * 129S6/SvEvTac * FVB/N * NIH Black Swiss

mortality/aging

decreased survivor rate ( J:138289 )

• only a few mice survive beyond 1 month of age

premature death ( J:138289 )

• most die within the first month of life

adipose tissue

adipose tissue phenotype ( J:138289 )

N • despite loss of white fat, brown fat is relatively unaffected

decreased white adipose tissue amount ( J:138289 )

• by 3 weeks of age all white adipose deposits are absent

behavior/neurological

behavior/neurological phenotype ( J:138289 )

N • no defect in eating behavior is detected, despite the absence of white fat

digestive/alimentary system

digestive/alimentary phenotype ( J:138289 )

N • no defect in fat absorption is detected, despite the absence of white fat

growth/size/body

decreased body size ( J:138289 )

• severely runted by 3 weeks of age

decreased body weight ( J:138289 )

homeostasis/metabolism

decreased circulating insulin-like growth factor I level ( J:138289 )

• by 21 days of age

decreased circulating leptin level ( J:138289 )

• by 21 days of age

decreased circulating growth hormone level ( J:138289 )

• by 21 days of age

decreased circulating triglyceride level ( J:138289 )

cellular

decreased cell proliferation ( J:138289 )

• by 3 weeks of age the proliferative index in most tissues is reduced

reproductive system

abnormal corpus luteum morphology ( J:138289 )

♀ • lack a well-developed corpus luteum

ovary hypoplasia ( J:138289 )

♀

testis hypoplasia ( J:138289 )

♂ • severe

female infertility ( J:138289 )

♀ • the few females that reach reproductive age are infertile

male infertility ( J:138289 )

♂ • the few males that reach reproductive age are infertile

skeleton

abnormal epiphyseal plate morphology ( J:138289 )

• at P21, long bone growth plates are severely dysplastic with many cells having mega-nuclei

endocrine/exocrine glands

abnormal adrenal cortex morphology ( J:138289 )

• the outer cortex lacks a functional zona fasciculata

abnormal corpus luteum morphology ( J:138289 )

♀ • lack a well-developed corpus luteum

ovary hypoplasia ( J:138289 )

♀

testis hypoplasia ( J:138289 )

♂ • severe

nervous system

abnormal brain size ( J:138289 )

• by 21 days of age there is a relative increase in the brain weight to body weight ratio

respiratory system

abnormal lung epithelium morphology ( J:138289 )

• decrease in the branching of the epithelium

Genotype
MGI:5473265

cx6

• Allelic Composition: E2f3^tm2.1Gle/E2f3^tm2.1Gle; E2f7^tm1.1Gle/E2f7^tm1.1Gle; E2f8^tm1.1Gle/E2f8^tm1.1Gle
• Genetic Background: involves: 129S6/SvEvTac

embryo

embryo phenotype ( J:183999 )

N • placentas appear normal with well-organized labyrinthine architecture and vasculature, and are functional as fetuses lack signs of acute stress response or ectopic cell apoptosis

N • live embryos/fetuses are recovered at all stages analyzed, including at birth