Phenotypes associated with this allele

• Allele Symbol: Tg(H2-K-MC159/EGFP)13Fkmc
• Allele Name: transgene insertion 13, Francis Ka-Ming Chan
• Allele ID: MGI:3806951
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(H2-K-MC159/EGFP)13Fkmc/Tg(H2-K-MC159/EGFP)13Fkmc	involves: C57BL/6	MGI:3806966
tg2	Tg(H2-K-MC159/EGFP)13Fkmc/0	involves: C57BL/6	MGI:3806965

Genotype
MGI:3806966

tg1

• Allelic Composition: Tg(H2-K-MC159/EGFP)13Fkmc/Tg(H2-K-MC159/EGFP)13Fkmc
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased T cell number ( J:138050 )

• mice exhibit an increase in T cell numbers compared to in wild-type mice

increased double-negative T cell number ( J:138050 )

• mice exhibit an increase in CD3+ B220+ CD4- CD8- alpha beta lineage double negative cells compared to in wild-type mice

• however, the increased T cell number is not due to an increase in dendritic cell number

increased IgG2b level ( J:138050 )

• slightly

increased anti-double stranded DNA antibody level ( J:138050 )

hematopoietic system

increased T cell number ( J:138050 )

• mice exhibit an increase in T cell numbers compared to in wild-type mice

increased double-negative T cell number ( J:138050 )

• mice exhibit an increase in CD3+ B220+ CD4- CD8- alpha beta lineage double negative cells compared to in wild-type mice

• however, the increased T cell number is not due to an increase in dendritic cell number

increased IgG2b level ( J:138050 )

• slightly

Genotype
MGI:3806965

tg2

• Allelic Composition: Tg(H2-K-MC159/EGFP)13Fkmc/0
• Genetic Background: involves: C57BL/6

immune system

immune system phenotype ( J:138050 )

N • despite defects in lymphoproliferation, immune response to infection with lymphocytic choriomeningitis virus (LCMV) is normal

decreased B cell apoptosis ( J:138050 )

• B cells are partially protected from CD95-induced apoptosis

• unlike in wild-type mice, CD4+ T cells are resistant to apoptosis induced by low doses but not high doses of anti-CD3

• however, lymphocytes are not protected from staurosporine-induced apoptosis of Bcl-2-regulated spontaneous lymphocyte death in the absence of external stimuli

decreased T cell apoptosis ( J:138050 )

• T cells are partially protected from CD95-induced apoptosis

• however, lymphocytes are not protected from staurosporine-induced apoptosis of Bcl-2-regulated spontaneous lymphocyte death in the absence of external stimuli

increased B cell proliferation ( J:138050 )

• mice exhibit mild B cell proliferation in secondary lymphoid organs that does not worsen with age

• however, mitogen-induced lymphoproliferation is normal

enlarged spleen ( J:138050 )

• 1.5- to 2-fold

abnormal lymphopoiesis ( J:138050 )

• proliferation of lymphocytes is increased compared to in wild-type mice and the ratio of B to T cells is shifted in favor of B cells in lymph nodes associated with an accumulation of mature follicular B cells

• however, lymphoproliferation stimulated by mitogens is normal

increased follicular B cell number ( J:138050 )

• mature follicular B cells accumulate to a greater extent than in wild-type mice

cellular

decreased sensitivity to induced cell death ( J:138050 )

• skin fibroblasts are resistant to TNG-induced apoptosis and programmed necrosis

decreased B cell apoptosis ( J:138050 )

• B cells are partially protected from CD95-induced apoptosis

• unlike in wild-type mice, CD4+ T cells are resistant to apoptosis induced by low doses but not high doses of anti-CD3

• however, lymphocytes are not protected from staurosporine-induced apoptosis of Bcl-2-regulated spontaneous lymphocyte death in the absence of external stimuli

decreased T cell apoptosis ( J:138050 )

• T cells are partially protected from CD95-induced apoptosis

• however, lymphocytes are not protected from staurosporine-induced apoptosis of Bcl-2-regulated spontaneous lymphocyte death in the absence of external stimuli

increased B cell proliferation ( J:138050 )

• mice exhibit mild B cell proliferation in secondary lymphoid organs that does not worsen with age

• however, mitogen-induced lymphoproliferation is normal

hematopoietic system

decreased B cell apoptosis ( J:138050 )

• B cells are partially protected from CD95-induced apoptosis

• unlike in wild-type mice, CD4+ T cells are resistant to apoptosis induced by low doses but not high doses of anti-CD3

• however, lymphocytes are not protected from staurosporine-induced apoptosis of Bcl-2-regulated spontaneous lymphocyte death in the absence of external stimuli

decreased T cell apoptosis ( J:138050 )

• T cells are partially protected from CD95-induced apoptosis

• however, lymphocytes are not protected from staurosporine-induced apoptosis of Bcl-2-regulated spontaneous lymphocyte death in the absence of external stimuli

increased B cell proliferation ( J:138050 )

• mice exhibit mild B cell proliferation in secondary lymphoid organs that does not worsen with age

• however, mitogen-induced lymphoproliferation is normal

enlarged spleen ( J:138050 )

• 1.5- to 2-fold

abnormal lymphopoiesis ( J:138050 )

• proliferation of lymphocytes is increased compared to in wild-type mice and the ratio of B to T cells is shifted in favor of B cells in lymph nodes associated with an accumulation of mature follicular B cells

• however, lymphoproliferation stimulated by mitogens is normal

increased follicular B cell number ( J:138050 )

• mature follicular B cells accumulate to a greater extent than in wild-type mice

growth/size/body

enlarged spleen ( J:138050 )

• 1.5- to 2-fold