Phenotypes associated with this allele

• Allele Symbol: Ulk1^tm1.1Thsn
• Allele Name: targeted mutation 1.1, Craig B Thompson
• Allele ID: MGI:3808004
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ulk1^tm1.1Thsn/Ulk1^tm1.1Thsn	B6.129-Ulk1^tm1.1Thsn	MGI:5554207
hm2	Ulk1^tm1.1Thsn/Ulk1^tm1.1Thsn	involves: 129S1/Sv * 129X1/SvJ	MGI:6287983
hm3	Ulk1^tm1.1Thsn/Ulk1^tm1.1Thsn	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3808024
cx4	Ulk1^tm1.1Thsn/Ulk1^tm1.1Thsn Ulk2^tm1.1Thsn/Ulk2^tm1.1Thsn	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:5141083

Genotype
MGI:5554207

hm1

• Allelic Composition: Ulk1^tm1.1Thsn/Ulk1^tm1.1Thsn
• Genetic Background: B6.129-Ulk1^tm1.1Thsn

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

vision/eye

vision/eye phenotype ( J:200084 )

N • electroretinogram responses (scotopic and photopic responses) in mutants are normal

Genotype
MGI:6287983

hm2

• Allelic Composition: Ulk1^tm1.1Thsn/Ulk1^tm1.1Thsn
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

immune system

immune system phenotype ( J:235399 )

N • mice do not develop lupus-like disease and do not exhibit an increase in anti-double stranded DNA antibodies or in anti-nuclear antibodies, do not show glomerular immune complex deposition, show normal serum creatinine levels and cytokine levels, and normal clearance of engulfed, dying cells

Genotype
MGI:3808024

hm3

• Allelic Composition: Ulk1^tm1.1Thsn/Ulk1^tm1.1Thsn
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

cellular

abnormal mitochondrial morphology ( J:139213 )

• an increase in mitochondria mass is noted both in erythrocytes and in mouse embryonic fibroblasts

abnormal autophagy ( J:139213 )

• defective autophagy in developing erythryocytes impairs their clearance of mitochondria and ribosomes

hematopoietic system

increased spleen weight ( J:139213 )

• spleen weight is increased by 1.7-fold

abnormal erythrocyte morphology ( J:139213 )

• there is about a 3-fold increase in the percentage of erythroid cells that contain mitochondria

abnormal erythropoiesis ( J:139213 )

• reticulocytes cultured in vitro are less able to clear mitochondria via autophagy during their development into erythrocytes

• none of the reticulocytes cultured for 18 hours have lost their mitochondria compared to over 20% of wild-type reticulocytes that lose their mitochondria

increased mean corpuscular hemoglobin concentration ( J:139213 )

• hemoglobulin concentration is increased both in mature red blood cells (17.1 pg vs. 15.5 pg in wild-type mice) and in reticulocytes (18.7 pg vs. 17.0 in wild-type)

increased mean corpuscular volume ( J:139213 )

• there is about a 10% increase in MCV that reflects a change in size for both reticulocytes and mature red blood cells

increased red blood cell distribution width ( J:139213 )

• distribution width of erythrocytes is increased from 12.1% in wild-type mice to 15.1% in these mutant mice

abnormal reticulocyte morphology ( J:139213 )

• distribution width of reticulocytes is significantly smaller than in controls (12.1% compared to 16.6% for wild-type reticulocytes)

reticulocytosis ( J:139213 )

• reticulocyte number in the blood is increased from 389.7 cells per liter in wild-type mice to 1735.7 cells per liter in mutant mice

immune system

increased spleen weight ( J:139213 )

• spleen weight is increased by 1.7-fold

growth/size/body

increased spleen weight ( J:139213 )

• spleen weight is increased by 1.7-fold

homeostasis/metabolism

abnormal autophagy ( J:139213 )

• defective autophagy in developing erythryocytes impairs their clearance of mitochondria and ribosomes

Genotype
MGI:5141083

cx4

• Allelic Composition: Ulk1^tm1.1Thsn/Ulk1^tm1.1Thsn; Ulk2^tm1.1Thsn/Ulk2^tm1.1Thsn
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

mortality/aging

neonatal lethality, complete penetrance ( J:174297 )

• mice die shortly after birth