Phenotypes associated with this allele

• Allele Symbol: Ikbkg^m1Btlr
• Allele Name: mutation 1, Bruce Beutler
• Allele ID: MGI:3808877
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Ikbkg^m1Btlr/Ikbkg^+	C57BL/6J-Ikbkg^m1Btlr	MGI:3808878
ot2	Ikbkg^m1Btlr/Y	C57BL/6J-Ikbkg^m1Btlr	MGI:3808880

Genotype
MGI:3808878

ht1

• Allelic Composition: Ikbkg^m1Btlr/Ikbkg⁺
• Genetic Background: C57BL/6J-Ikbkg^m1Btlr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reduced female fertility ( J:139558 )

♀ • female mice heterozygous for this mutation are poor breeders

Genotype
MGI:3808880

ot2

• Allelic Composition: Ikbkg^m1Btlr/Y
• Genetic Background: C57BL/6J-Ikbkg^m1Btlr

cellular

azoospermia ( J:139558 )

♂

mortality/aging

mortality/aging ( J:139558 )

♂N • despite earlier reports that hemizygous males are born at a lower-than-expected frequency, larger numbers of births showed that hemizygotes are born at the expected Mendelian ratio; they survive for at least 4-6 weeks

reproductive system

azoospermia ( J:139558 )

♂

immune system

immune system phenotype ( J:139558 )

♂N • type 1 interferon (IFN) production by macrophages from hemizygous mutant male mice in response to the toll-like receptor (TLR) 3 ligand poly I:C (a dsRNA mimetic) and to dsDNA is normal

♂N • mutant mice have normal CD8⁺ lymphocyte function

decreased regulatory T cell number ( J:139558 )

♂ • mutant mice have a two-fold reduction in peripheral CD4⁺CD25⁺ lymphocytes (regulatory T cells)

decreased tumor necrosis factor secretion ( J:139558 )

♂ • upon stimulation with the TLR4 ligand, lipopolysaccharide (LPS), peritoneal macrophages from hemizygous mutant male mice exhibit a subtly reduced TNF-alpha response compared to that of wild-type mice

♂ • mutant macrophages produce normal amounts of tumor necrosis factor (TNF) alpha in response to stimulation with all other toll-like receptor (TLR) ligands tested: the TLR3 ligand poly(I:C); the TLR1/2 ligand Pam3CSK4; the TLR7 ligand resiquimod; the TLR9 ligand CpG ODN; and the TLR6 ligands peptidoglycan and MALP-2

hematopoietic system

decreased regulatory T cell number ( J:139558 )

♂ • mutant mice have a two-fold reduction in peripheral CD4⁺CD25⁺ lymphocytes (regulatory T cells)