Analysis Tools
immune system
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• the heavy chain encoded by the knocked-in allele is paired with a very limited repertoire of kappa light chains with the majority of these being from the Vkappa38c family
• many of these kappa light chains disrupt the intrinsic DNA-binding ability of the heavy chain so that relatively few B cells outside the bone marrow produce antibodies that bind double-stranded DNA
• sequence analysis reveals most B cells have undergone receptor rearrangement multiple times with a bias towards Jkappa5 occurring, deletion of Ckappa often observed, and a high frequency of deletional and inverted rearrangements
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• B cell hybridomas developed from these mice produce antibodies that bind dsDNA
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hematopoietic system
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• the heavy chain encoded by the knocked-in allele is paired with a very limited repertoire of kappa light chains with the majority of these being from the Vkappa38c family
• many of these kappa light chains disrupt the intrinsic DNA-binding ability of the heavy chain so that relatively few B cells outside the bone marrow produce antibodies that bind double-stranded DNA
• sequence analysis reveals most B cells have undergone receptor rearrangement multiple times with a bias towards Jkappa5 occurring, deletion of Ckappa often observed, and a high frequency of deletional and inverted rearrangements
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