Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze}
• Allele Name: targeted mutation 3, Hongkui Zeng
• Allele ID: MGI:3809521
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Gt(ROSA)26Sor^tm3(CAG-EYFP)Hze/Gt(ROSA)26Sor^+	involves: 129S6/SvEvTac * C57BL/6NCr	MGI:4436849
cn2	Gt(ROSA)26Sor^tm3(CAG-EYFP)Hze/Gt(ROSA)26Sor^+ Hira^tm1c(EUCOMM)Wtsi/Hira^tm1d(EUCOMM)Wtsi Tg(Myh6-cre)2182Mds/0	B6.Cg-Gt(ROSA)26Sor^tm3(CAG-EYFP)Hze Hira^tm1c(EUCOMM)Wtsi/Hira^tm1d(EUCOMM)Wtsi Tg(Myh6-cre)2182Mds	MGI:5823159
cn3	Gt(ROSA)26Sor^tm3(CAG-EYFP)Hze/Gt(ROSA)26Sor^+ Stat5a^tm2Mam Stat5b^tm1Mam/Del(11Stat5a-Stat5b)1Mam Tg(Mx1-cre)1Cgn/0	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NCr * CBA	MGI:5544442

Genotype
MGI:4436849

ht1

• Allelic Composition: Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NCr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:155793 )

• mice are viable and fertile; used for cre reporter expression analysis

Genotype
MGI:5823159

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze}/Gt(ROSA)26Sor⁺; Hira^{tm1c(EUCOMM)Wtsi}/Hira^{tm1d(EUCOMM)Wtsi}; Tg(Myh6-cre)2182Mds/0
• Genetic Background: B6.Cg-Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze} Hira^{tm1c(EUCOMM)Wtsi}/Hira^{tm1d(EUCOMM)Wtsi} Tg(Myh6-cre)2182Mds
• Cell Lines: EPD0181_1_A05

cardiovascular system

abnormal heart morphology ( J:231593 )

• however, heart development proceeds normally

• hearts show re-expression of fetal cardiac genes

abnormal myocardial fiber morphology ( J:231593 )

• hypertrophic cardiomyocytes are seen in the right ventricle free wall as early as 15 days after birth

• however, aberrant proliferation of or apoptosis of cardiomyocytes is not seen and there is no evidence of increased DNA damage

myocardial fiber degeneration ( J:231593 )

• lesions contain degenerating cardiomyocytes

cardiac fibrosis ( J:231593 )

• hearts at 6 weeks and 6 months of age exhibit white surface scars localized to the subepicardial region of the ventricular free walls; lesions contain degenerating cardiomyocytes and a large degree of collagen deposition indicating focal replacement fibrosis localized to the subepicardial myocardium

abnormal cardiovascular system physiology ( J:231593 )

• mice show increased arterial elastance at 6 months of age, which is a measure of arterial load

• the slope of the end-diastolic pressure-volume relationship, which describes diastolic function, is elevated

• however, no effect is seen on the slope of end-systolic pressure-volume relationship, which describes the maximal developed ventricular pressure at any given ventricular volume

abnormal blood circulation ( J:231593 )

• mice show decreased stroke work at 6 months

decreased cardiac output ( J:231593 )

• at 6 months of age

decreased cardiac stroke volume ( J:231593 )

• at 6 months of age

abnormal cardiac muscle contractility ( J:231593 )

• mice exhibit increased dp/dtmax-end-diastolic volume, the relationship between peak rate of pressure rise and EDV, which describes ventricular contractile performance

abnormal myocardial fiber physiology ( J:231593 )

• mice show an increase in diastolic myocardial stiffness

congestive heart failure ( J:231593 )

cellular

oxidative stress ( J:231593 )

• levels of manganese superoxide dismutase are reduced in scar-containing free-wall right ventricle, but not in non-scarred regions, suggesting impaired response to oxidative stress

• however, only very minor increases in reactive oxygen species are seen, indicating that cardiomyocyte degeneration most likely is not caused by increased oxidative stress

muscle

abnormal myocardial fiber morphology ( J:231593 )

• hypertrophic cardiomyocytes are seen in the right ventricle free wall as early as 15 days after birth

• however, aberrant proliferation of or apoptosis of cardiomyocytes is not seen and there is no evidence of increased DNA damage

myocardial fiber degeneration ( J:231593 )

• lesions contain degenerating cardiomyocytes

abnormal cardiac muscle contractility ( J:231593 )

• mice exhibit increased dp/dtmax-end-diastolic volume, the relationship between peak rate of pressure rise and EDV, which describes ventricular contractile performance

abnormal sarcolemma morphology ( J:231593 )

• cardiomyocyte sarcolemmal integrity is compromised in focal subepicardial areas between 15 and 25 days after birth

Genotype
MGI:5544442

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze}/Gt(ROSA)26Sor⁺; Stat5a^tm2Mam Stat5b^tm1Mam/Del(11Stat5a-Stat5b)1Mam; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NCr * CBA

hematopoietic system

abnormal CD4-positive T cell differentiation ( J:204829 )

• fewer IL9+ cells in IFN-alpha-treated mice following differentiation towards Th9 lineage

immune system

abnormal CD4-positive T cell differentiation ( J:204829 )

• fewer IL9+ cells in IFN-alpha-treated mice following differentiation towards Th9 lineage