Phenotypes associated with this allele

• Allele Symbol: Kif5b^tm1Njen
• Allele Name: targeted mutation 1, Nancy A Jenkins
• Allele ID: MGI:3809821
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kif5b^tm1Njen/Kif5b^tm1Njen	involves: 129S1/Sv	MGI:4949222
cn2	Kif5b^tm1Njen/Kif5b^tm1.1Njen Tg(Ins2-cre)25Mgn/0	involves: 129S1/Sv * C57BL/6 * C57BL/6N * DBA * SJL	MGI:4946372
cn3	Kif5b^tm1Njen/Kif5b^+ Tg(Ins2-cre)25Mgn/0	involves: 129S1/Sv * C57BL/6 * C57BL/6N * DBA * SJL	MGI:4946373

Genotype
MGI:4949222

hm1

• Allelic Composition: Kif5b^tm1Njen/Kif5b^tm1Njen
• Genetic Background: involves: 129S1/Sv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal vesicle-mediated transport ( J:170887 )

• cre-treated neurons exhibit impaired retrograde vesicle transport compared with wild-type cells

• however, neurons exhibit normal anterograde

Genotype
MGI:4946372

cn2

• Allelic Composition: Kif5b^tm1Njen/Kif5b^tm1.1Njen; Tg(Ins2-cre)25Mgn/0
• Genetic Background: involves: 129S1/Sv * C57BL/6 * C57BL/6N * DBA * SJL

endocrine/exocrine glands

abnormal pancreatic beta cell morphology ( J:170198 )

• mitochondria cluster in the perinuclear region unlike in wild-type beta cells

increased pancreatic beta cell number ( J:170198 )

small pancreatic islets ( J:170198 )

abnormal pancreas physiology ( J:170198 )

• islet cell proliferation is decreased compared to in control mice

• however, the rate of apoptosis is normal

abnormal pancreatic beta cell physiology ( J:170198 )

• mice exhibit an increase in insulin vesicles per cell area compared to in wild-type cells

decreased insulin secretion ( J:170198 )

• in early and slow phase after glucose injection

• after KCl-stimulation of islets

• however, insulin secretion is normal in the absence of glucose stimulation

homeostasis/metabolism

decreased insulin secretion ( J:170198 )

• in early and slow phase after glucose injection

• after KCl-stimulation of islets

• however, insulin secretion is normal in the absence of glucose stimulation

hyperglycemia ( J:170198 )

• progressive increase in random and 16-hour fasting conditions

decreased circulating insulin level ( J:170198 )

• in fasting conditions, but not in a random fed state

impaired glucose tolerance ( J:170198 )

• mice exhibit impaired glucose intolerance compared with wild-type mice

• however, blood glucose drops to wild-type levels 15 minutes after insulin injection

growth/size/body

decreased body weight ( J:170198 )

postnatal growth retardation ( J:170198 )

Genotype
MGI:4946373

cn3

• Allelic Composition: Kif5b^tm1Njen/Kif5b⁺; Tg(Ins2-cre)25Mgn/0
• Genetic Background: involves: 129S1/Sv * C57BL/6 * C57BL/6N * DBA * SJL

homeostasis/metabolism

decreased insulin secretion ( J:170198 )

• slight after glucose injection

impaired glucose tolerance ( J:170198 )

• slight

endocrine/exocrine glands

decreased insulin secretion ( J:170198 )

• slight after glucose injection