Phenotypes associated with this allele

• Allele Symbol: Tg(tetO-Kdr*)4377.5Rwng
• Allele Name: transgene insertion 4377.5, Rong Wang
• Allele ID: MGI:3810550
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Kdr^tm1Jrt/Kdr^+ Tg(tetO-Kdr*)4377.5Rwng/0 Tg(Cebpb-tTA)5Bjd/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N * NMRI	MGI:3810713
cx2	Tg(tetO-Kdr*)4377.5Rwng/0 Tg(Cebpb-tTA)5Bjd/0	involves: FVB/N * NMRI	MGI:3810712

Genotype
MGI:3810713

cx1

• Allelic Composition: Kdr^tm1Jrt/Kdr⁺; Tg(tetO-Kdr*)4377.5Rwng/0; Tg(Cebpb-tTA)5Bjd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N * NMRI

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

liver/biliary system

abnormal liver development ( J:100427 )

• at E12.5, liver vasculature is less organized and vessels are dilated

• at E13.5, microvascular network is more disorganized with fewer branches than in control livers; newborns have very little microvascular network is observed

Genotype
MGI:3810712

cx2

• Allelic Composition: Tg(tetO-Kdr*)4377.5Rwng/0; Tg(Cebpb-tTA)5Bjd/0
• Genetic Background: involves: FVB/N * NMRI

mortality/aging

perinatal lethality, incomplete penetrance ( J:100427 )

• between 25% and 38% of pups are stillborn

liver/biliary system

liver/biliary system phenotype ( J:100427 )

N • liver glycogen and albumin levels are comparable to control animals

abnormal liver morphology ( J:100427 )

• livers from neonates display a decrease in blood vessels

• livers of all newborn animals are dark red in appearance in contrast to pink color of control livers

• fewer endothelial cells (ECs) are present in mutant liver vasculature; ECs are present but are less organized and more discontinuous than in controls

• fewer or collapsed-appearing sinusoidal channels are observed

• livers have a sparse and poorly developed sinusoidal network with numerous blood cells in the disrupted sinusoidal spaces

• parenchymal cells contain very few or no lipid droplets

• morphology of space of Disse is abnormal, showing large gaps between sinusoidal epithelial cells (SECs) and hepatocytes

• sinusoidal epithelium lacks fenestrations seen in normal livers

abnormal hepatocyte morphology ( J:100427 )

• hepatocytes are more scattered than in control livers and many are oddly shaped with fewer microvilli projections into the space of Disse

abnormal liver physiology ( J:100427 )

• there is increase in red blood cells in liver due to defective circulation

• livers show a significant defect in lipoprotein uptake compared to controls

jaundice ( J:100427 )

• 30 to 63% of newborns are jaundiced; suppression of transgenic Kdr by doxycycline treatement of dams during embryogenesis eliminates jaundice that is observed in untreated animals

cardiovascular system

abnormal blood vessel morphology ( J:100427 )

• decreased number of blood vessels in liver

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:100427 )

N • serum bilirubin levels are normal