Phenotypes associated with this allele

• Allele Symbol: Tg(CMV-Serpine1)1Dgi
• Allele Name: transgene insertion 1, David Ginsburg
• Allele ID: MGI:3810927
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Apoe^tm1Unc/Apoe^tm1Unc Tg(CMV-Serpine1)1Dgi/0	B6.Cg-Apoe^tm1Unc Tg(CMV-Serpine1)1Dgi	MGI:3810982
cx2	Ldlr^tm1Her/Ldlr^tm1Her Tg(CMV-Serpine1)1Dgi/0	B6.Cg-Ldlr^tm1Her Tg(CMV-Serpine1)1Dgi	MGI:3810981
tg3	Tg(CMV-Serpine1)1Dgi/0	B6.Cg-Tg(CMV-Serpine1)1Dgi	MGI:3810980
tg4	Tg(CMV-Serpine1)1Dgi/0	involves: C57BL/6J * SJL/J	MGI:3810979

Genotype
MGI:3810982

cx1

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Tg(CMV-Serpine1)1Dgi/0
• Genetic Background: B6.Cg-Apoe^tm1Unc Tg(CMV-Serpine1)1Dgi

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

atherosclerotic lesions ( J:61287 )

• when fed a high fat Western diet for 6-30 weeks, mice develop atherosclerotic aortic lesions of the intimal and medial areas; incidence and severity are similar for Serpine1-deficient or transgenic Apoe-deficient genotypes

• lesions are somewhat larger than those observed on the Ldlr-deficient background

• cellular composition of lesions is similar among all Serpine1-deficient or transgenic, Apoe-deficient genotypes, or Apoe-deficient only mice; at early time points, a greater foam cell content is observed, with more prominent cholesterol clefts and necrotic areas evident with increasing age

homeostasis/metabolism

increased circulating cholesterol level ( J:61287 )

• mice develop severe cholesterolemia when receiving a high fat diet from 6 to 30 weeks (elevated levels are detected at 6, 15, and 30 weeks)

Genotype
MGI:3810981

cx2

• Allelic Composition: Ldlr^tm1Her/Ldlr^tm1Her; Tg(CMV-Serpine1)1Dgi/0
• Genetic Background: B6.Cg-Ldlr^tm1Her Tg(CMV-Serpine1)1Dgi

cardiovascular system

atherosclerotic lesions ( J:61287 )

• when fed a high fat Western diet for 6-30 weeks, mice develop atherosclerotic aortic lesions of the intimal and medial areas; incidence and severity are similar for Serpine1-deficient or transgenic Ldlr-deficient genotypes

homeostasis/metabolism

increased circulating cholesterol level ( J:61287 )

• mice develop severe cholesterolemia receiving a high fat diet from 6 to 30 weeks (elevated levels are detected at 6, 15, and 30 weeks)

Genotype
MGI:3810980

tg3

• Allelic Composition: Tg(CMV-Serpine1)1Dgi/0
• Genetic Background: B6.Cg-Tg(CMV-Serpine1)1Dgi

cardiovascular system

cardiovascular system phenotype ( J:61287 )

N • similar to wild-type controls, after 30 weeks of receiving a high fat Western diet, transgenic mice do not display any atherosclerotic lesions

Genotype
MGI:3810979

tg4

• Allelic Composition: Tg(CMV-Serpine1)1Dgi/0
• Genetic Background: involves: C57BL/6J * SJL/J

respiratory system

abnormal lung morphology ( J:31542 )

• bleomycin-induced increase in lung collagen content (determined from hydroxyproline content) is 3.2 fold greater than that in PBS-treated wild-type mice and significantly greater (1.9 fold greater) than that in bleomycin treated wild-type mice at 2 weeks post treatment

• lung fibrin deposition following bleomycin treatment is significantly increased compared to treated wild-type mice

pulmonary fibrosis ( J:31542 , J:140007 )

• mice exhibit increased susceptibility to pulmonary fibrosis following injury or intratracheal induction with bleomycin (J:140007)

neoplasm

neoplasm ( J:33232 )

N • no significant differences are observed compared to controls in terms of primary tumor size of footpad tumors induced by foot pad injection with tumor cells at 10, 17, or 21 days post-injection

N • after injection of melanoma cells, number of primary pulmonary metastases post-injection, or tumor burdens initiated by injections of increasing doses of cells do not significantly differ from wild-type controls

N • survival times after foot pad tumor removal are not significantly different among Serpine1-deficient, Serpine1-overexpressing transgenic, or wild-type mice