Phenotypes associated with this allele

• Allele Symbol: Inpp5k^tm1Brd
• Allele Name: targeted mutation 1, Allan Bradley
• Allele ID: MGI:3810936
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Inpp5k^tm1Brd/Inpp5k^tm1Brd	B6.Cg-Inpp5k^tm1Brd	MGI:3810965
ht2	Inpp5k^tm1Brd/Inpp5k^+	B6.Cg-Inpp5k^tm1Brd	MGI:3810966

Genotype
MGI:3810965

hm1

• Allelic Composition: Inpp5k^tm1Brd/Inpp5k^tm1Brd
• Genetic Background: B6.Cg-Inpp5k^tm1Brd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:139745 )

• no mutant embryos were identified after embryonic day 10.5

Genotype
MGI:3810966

ht2

• Allelic Composition: Inpp5k^tm1Brd/Inpp5k⁺
• Genetic Background: B6.Cg-Inpp5k^tm1Brd

growth/size/body

decreased susceptibility to diet-induced obesity ( J:139745 )

• after 6 weeks on a high fat diet, mice gained 30.9% of their body weight, whereas wild-type mice fed the same diet gained 41.4% of their body weight

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:139745 )

• after 6 weeks on a high fat diet, mice gained 30.9% of their body weight, whereas wild-type mice fed the same diet gained 41.4% of their body weight

abnormal blood homeostasis ( J:139745 )

decreased circulating glucose level ( J:139745 )

• fasting glucose levels are significantly lower for both sexes (116.8 mg/dl versus 92.6 mg/dl in wild-type for male and similar results for female mice)

decreased circulating insulin level ( J:139745 )

• mice fed a high-fat diet have lower insulin levels than controls ( 2.77 ng/ml vs. 3.49 ng/ml)

decreased circulating leptin level ( J:139745 )

• mice fed a high fat diet have lower circulating leptin levels ( 4.40 ng/ml vs. 7.72 ng/ml)

decreased circulating free fatty acids level ( J:139745 )

• fasting cholesterol levels are significantly lower (0.80 meq/liter versus 0.52 meq/liter in wild-type for male mice)

decreased circulating triglyceride level ( J:139745 )

• fasting triglyceride levels are significantly lower (100.5 mg/dl versus 142.3 mg/dl in wild-type for male mice)

improved glucose tolerance ( J:139745 )

• blood glucose levels are significantly lower 60 and 90 minutes after glucose injection compared to control mice

• mice fed a high fat diet have lower levels of blood glucose than controls (aprox 160 mg/dl vs. 210 mg/dl)

increased insulin sensitivity ( J:139745 )

• blood glucose levels are significantly lower than controls for the first hour after administration

• the rate of insulin stimulated glucose disposal is also significantly elevated in hyperinsulinemic-euglycemic clamp analysis

decreased cholesterol level ( J:139745 )

muscle

increased skeletal muscle cell glucose uptake ( J:139745 )

• incorporation of radio-labeled glucose is enhanced in skeletal muscle when given insulin compared to wild-type muscle

increased skeletal muscle mass ( J:139745 )

• there is a slight but significant increase in quadricep muscle mass to total body mass ratio

cellular

increased skeletal muscle cell glucose uptake ( J:139745 )

• incorporation of radio-labeled glucose is enhanced in skeletal muscle when given insulin compared to wild-type muscle