All Phenotypes Ccdc87<tm1(KOMP)Vlcg> MGI Mouse

reproductive system phenotype ( J:265624 )

N	• male homozygotes exhibit no differences in testis weight, shape and histology, epididymis weight, sperm counts in cauda epididymides, and sperm flagellum morphology relative to wild-type controls

abnormal sperm head morphology ( J:265624 )

• ~20% of sperm from the testis, caput, and cauda epididymides exhibit severe head defects

abnormal acrosome assembly ( J:265624 )

• ~20% of sperm from the testis and epididymis exhibit abnormal acrosome formation

abnormal sperm nucleus morphology ( J:265624 )

• ~20% of sperm from the testis and epididymis exhibit impaired cell nucleus formation

abnormal sperm motility ( J:265624 )

• sperm from the cauda epididymides exhibit a significant reduction in the initial motility after isolation

• however, no defects in sperm motility are observed during capacitation

male infertility ( J:265624 )

• 8 of 10 male homozygotes are completely sterile

reduced male fertility ( J:265624 )

• 2 of 10 male homozygotes are subfertile

impaired fertilization ( J:265624 )

• although sperm adhere to and accumulate in the perivitelline space of intact eggs, their fertilizing capacity is significantly reduced, indicating failure of sperm-egg interaction

impaired acrosome reaction ( J:265624 )

• sperm exhibit significantly decreased capacity for spontaneous and progesterone (P4)-induced acrosome reactions

decreased fertilization frequency ( J:265624 )

• in vitro fertilization analysis of epididymal sperm using intact eggs revealed a significant decrease in the rate of 2-cell embryo formation