All Phenotypes Fads2<tm1Wst> MGI Mouse

homeostasis/metabolism phenotype ( J:140341 )

N	• despite absence of long-chain polyunsaturated fatty acids lipid and carbohydrate metabolism are similar to controls

abnormal platelet physiology ( J:140341 )

• fail to synthesize and secrete thromboxane A2

• addition of arachidonic acid restores thromboxane A2 synthesis and secretion

• arachidonic acid dietary supplementation restores normal platelet function

decreased platelet aggregation ( J:140341 )

• fail to aggregate in aggregation assays

decreased susceptibility to induced thrombosis ( J:140341 )

• failure of thrombus formation following application of FeCl3 to the adventia of the common carotid artery

increased bleeding time ( J:140341 )

• despite normal platelet numbers, bleeding times are about twice as long as in controls

• arachidonic acid supplementation restores normal bleeding times

decreased unsaturated fatty acids level ( J:140341 )

• long-chain polyunsaturated fatty acids are absent from total lipid extracts of liver, serum lipoproteins, ovary, testes, adrenals, and retina

decreased prostaglandin level ( J:140341 )

• total PGE2 concentrations in epididymal extracts are only 5-10 ng/epididymis compared to 660-1100 ng/epididymis in controls

• serum PGE2 concentrations range from 3 - 11 ng/ml compared to 57 - 76 ng/ml in controls

decreased thromboxane level ( J:140341 )

• platelets fail to synthesize and secrete thromboxane A2 in aggregation assays

abnormal ovarian follicle morphology ( J:140341 )

abnormal granulosa cell morphology ( J:140341 )

• the multilayer granulosa cell syncytium is absent

• marker analysis indicates that gap junctions are completely disordered

• gap junctions are barely detectable by electron microscopic analysis

absent mature ovarian follicles ( J:140341 )

impaired ovarian folliculogenesis ( J:140341 )

• folliculogenesis is arrested

• the strong blood supply seen during the cycle in controls is never seen in mutants

• the multilayer granulosa cell syncytium, theca folliculi, and zona pellucida are absent or in some cases the zona pellucida is present but poorly developed

• when the diet is supplemented with arachidonic acid or eicosapentaenoic / docosahexaenoic acids both prenatally and postnatally folliculogenesis is restored

absent theca folliculi ( J:140341 )

abnormal testis morphology ( J:140341 )

abnormal Sertoli cell morphology ( J:140341 )

• irregular distribution of markers indicates disruption of cell polarity, cell-cell tight junctions, and the blood testes barrier

• lipid polarity of the cell membrane is disturbed with the filipin-cholesterol complex distributed throughout the basolateral and apical compartments

decreased testis weight ( J:140341 )

• weight is about 2/3 that of controls

abnormal gametogenesis ( J:140341 )

abnormal oocyte morphology ( J:140341 )

abnormal zona pellucida morphology ( J:140341 )

• in some cases the zona pellucida is present but poorly developed

absent zona pellucida ( J:140341 )

• the zona pellucida is absent

azoospermia ( J:140341 )

arrest of spermiogenesis ( J:140341 )

• spermatids fail to complete acrosome and tail formation and arrest at the round haploid spermatid stage

• when the diet is supplemented with arachidonic acid or eicosapentaenoic / docosahexaenoic acids both prenatally and postnatally spermiogenesis is restored

abnormal reproductive system physiology ( J:140341 )

abnormal testis physiology ( J:140341 )

• lanthanum nitrate and fluorescent dyes freely cross the blood testes barrier

female infertility ( J:140341 )

• infertile when on a normal diet

• when the diet is supplemented with arachidonic acid or eicosapentaenoic / docosahexaenoic acids both prenatally and postnatally mice are fertile

male infertility ( J:140341 )