Phenotypes associated with this allele

• Allele Symbol: St6gal1^tm1Jlau
• Allele Name: targeted mutation 1, Joseph TY Lau
• Allele ID: MGI:3814350
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	St6gal1^tm1Jlau/St6gal1^tm1Jlau	129S4/SvJae-St6gal1^tm1Jlau	MGI:3814360
hm2	St6gal1^tm1Jlau/St6gal1^tm1Jlau	B6.129S4-St6gal1^tm1Jlau	MGI:3814373

Genotype
MGI:3814360

hm1

• Allelic Composition: St6gal1^tm1Jlau/St6gal1^tm1Jlau
• Genetic Background: 129S4/SvJae-St6gal1^tm1Jlau

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal neutrophil physiology ( J:140702 )

• neutrophil recruitment into the peritoneum is increased with about 2- fold higher neutrophil numbers than controls 18 hours after bacterial infection

• the majority of these neutrophils express high levels of the Gr-1 surface marker, which is indicative of a mature phenotype

• the number of mature neutrophils recruited by bacterial infection is five-fold greater than in wild-type

increased susceptibility to bacterial infection ( J:140702 )

• six days after intraperitoneal inoculation with 5 x 10⁵ CFUs of salmonella, mice have a 14-fold higher bacterial count in the liver and a 7-fold higher count in the spleen than do wild-type controls

• spleno- and hepatomegaly result by 6 days after bacterial inoculation compared to controls

hematopoietic system

abnormal neutrophil physiology ( J:140702 )

• neutrophil recruitment into the peritoneum is increased with about 2- fold higher neutrophil numbers than controls 18 hours after bacterial infection

• the majority of these neutrophils express high levels of the Gr-1 surface marker, which is indicative of a mature phenotype

• the number of mature neutrophils recruited by bacterial infection is five-fold greater than in wild-type

Genotype
MGI:3814373

hm2

• Allelic Composition: St6gal1^tm1Jlau/St6gal1^tm1Jlau
• Genetic Background: B6.129S4-St6gal1^tm1Jlau

immune system

peritoneal inflammation ( J:140547 )

• intraperitoneal thioglycollate elicitation recruits double the number of leukocyte cells compared to controls both 5 hours and one day after elicitation

• by 80 hours after elicitation, peritoneal cell counts in the mutant animals were only 20% greater than in their control counterparts

• differences in cell numbers recruited by intraperitoneal thioglycollate injection only occur at higher doses with no differences being observed at low doses

abnormal myelopoiesis ( J:140547 )

• there is a 30% increase in the percentage of myeloid cells in the blood that are actively proliferating

• mice have 1.6- to 2-fold greater granulocyte counts than controls 5 to 6 days after cyclophosphamide treatment is given to induce neutropenia

• there are increased numbers of granulocyte colony forming units in the bone marrow of mice compared to wild-type controls (2.82 vs. 1.9 per 1000 cells)

increased neutrophil cell number ( J:140547 )

• 4 hours after intraperitoneal thioglycollate elicitation, there is a 2.5-fold elevation of granulocyte/neutrophils numbers in circulation

• mice have 60% more neutrophils in the blood than controls when epinephrine is administered to release neutrophils from blood vessel walls

• the differences in epinephrine-induced neutrophilia disappear after 2 hours of administration

• likewise, stimulating the release of neutrophils from the bone marrow by G-CSF administration leads to twice as many neutrophils in circulation as controls

abnormal neutrophil physiology ( J:140547 )

• only 18% of neutrophils have apoptotic nuclei 24 hours after thioglycollate elicitation compared to 30% in wild-type controls

• early signs of apoptosis (i.e. membrane permeability) are also decreased in these neutrophils

digestive/alimentary system

peritoneal inflammation ( J:140547 )

• intraperitoneal thioglycollate elicitation recruits double the number of leukocyte cells compared to controls both 5 hours and one day after elicitation

• by 80 hours after elicitation, peritoneal cell counts in the mutant animals were only 20% greater than in their control counterparts

• differences in cell numbers recruited by intraperitoneal thioglycollate injection only occur at higher doses with no differences being observed at low doses

hematopoietic system

abnormal myelopoiesis ( J:140547 )

• there is a 30% increase in the percentage of myeloid cells in the blood that are actively proliferating

• mice have 1.6- to 2-fold greater granulocyte counts than controls 5 to 6 days after cyclophosphamide treatment is given to induce neutropenia

• there are increased numbers of granulocyte colony forming units in the bone marrow of mice compared to wild-type controls (2.82 vs. 1.9 per 1000 cells)

increased neutrophil cell number ( J:140547 )

• 4 hours after intraperitoneal thioglycollate elicitation, there is a 2.5-fold elevation of granulocyte/neutrophils numbers in circulation

• mice have 60% more neutrophils in the blood than controls when epinephrine is administered to release neutrophils from blood vessel walls

• the differences in epinephrine-induced neutrophilia disappear after 2 hours of administration

• likewise, stimulating the release of neutrophils from the bone marrow by G-CSF administration leads to twice as many neutrophils in circulation as controls

abnormal neutrophil physiology ( J:140547 )

• only 18% of neutrophils have apoptotic nuclei 24 hours after thioglycollate elicitation compared to 30% in wild-type controls

• early signs of apoptosis (i.e. membrane permeability) are also decreased in these neutrophils