Phenotypes associated with this allele

• Allele Symbol: Arhgdib^tm1Miyo
• Allele Name: targeted mutation 1, Jun Miyoshi
• Allele ID: MGI:3814585
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Arhgdib^tm1Miyo/Arhgdib^tm1Miyo	involves: 129S/SvEv	MGI:3814725
cx2	Arhgdia^tm1Ytk/Arhgdia^tm1Ytk Arhgdib^tm1Miyo/Arhgdib^tm1Miyo	involves: 129S/SvEv	MGI:3814726

Genotype
MGI:3814725

hm1

• Allelic Composition: Arhgdib^tm1Miyo/Arhgdib^tm1Miyo
• Genetic Background: involves: 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased B cell number ( J:140671 )

• a reduction occurs in the number of B cells found in the bone marrow of 18 week old mice is observed

decreased T cell number ( J:140671 )

• the number of T cells found in the bone marrow of 18 week old mice is reduced

• there is a 44% reduction in the number of circulating T cells found in adults

reproductive system

abnormal spermatogenesis ( J:140671 )

♂ • mild spermatogenesis defects are noted in the testis of 16 week old mice but fertility is not affected

hematopoietic system

decreased B cell number ( J:140671 )

• a reduction occurs in the number of B cells found in the bone marrow of 18 week old mice is observed

decreased T cell number ( J:140671 )

• the number of T cells found in the bone marrow of 18 week old mice is reduced

• there is a 44% reduction in the number of circulating T cells found in adults

Genotype
MGI:3814726

cx2

• Allelic Composition: Arhgdia^tm1Ytk/Arhgdia^tm1Ytk; Arhgdib^tm1Miyo/Arhgdib^tm1Miyo
• Genetic Background: involves: 129S/SvEv

mortality/aging

decreased survivor rate ( J:140671 )

• though 75% of mice die during embryogenesis, mice born live at least until 12 months of age

prenatal lethality, incomplete penetrance ( J:140671 )

• 75% of mice die during embryogenesis

immune system

enhanced B cell migration ( J:140671 )

• splenic B cells have a slightly enhanced migratory response to the chemokine CXCL12

increased B cell proliferation ( J:140671 )

• B cells activated in vitro with LPS have significantly higher proliferation rates

abnormal thymus medulla morphology ( J:140671 )

• mature CD4⁺ T cells accumulate in the medulla of the thymus

abnormal leukocyte cell number ( J:140671 )

decreased immature B cell number ( J:140671 )

• immature B cells numbers are reduced by almost half in the bone marrow of adult mice

• newly formed B cells are reduced by 20-30% in the spleen of adult mice

decreased transitional stage B cell number ( J:140671 )

• T1 and T2 transitional B cells are reduced by 20-30% in the spleen of adult mice

increased eosinophil cell number ( J:140671 )

• the proportion of eosinophils in the bone marrow is three times higher than in controls

• increased numbers of eosinophils are also observed in the lung

• these increase numbers correlate with an increased amount of circulating IL-5

• these eosinophil differences are more clearly observed between the 12- to 55-wk-old groups than between the 6- and 11-wk-old groups

decreased mature B cell number ( J:140671 )

• mature B cell numbers are reduced by more than half in the bone marrow of adult mice

decreased follicular B cell number ( J:140671 )

• follicular B cells are reduced by 20-30% in the spleen of adult mice

decreased marginal zone B cell number ( J:140671 )

• MZ B cells are virtually absent in the spleen of adult mice

decreased pre-B cell number ( J:140671 )

• pre-B cell numbers are reduced in the bone marrow to varying degrees depending on age

• numbers are reduced by about 25% at 7 weeks of age and by over 50% at 12-32 weeks of age

decreased T cell number ( J:140671 )

• there is a 80-90% reduction in the number of circulating T cells found in adults

decreased double-positive T cell number ( J:140671 )

• the percentage of double-positive thymocytes is reduced by 30% compared to controls at 18 weeks of age

increased single-positive T cell number ( J:140671 )

• the percentages of single-positive thymocytes are increased 2-fold

spleen hypoplasia ( J:140671 )

• spleen cellularity is reduced in these mice

disorganized spleen B cell follicle ( J:140671 )

• localization of B cells is disorganized in the border region of the white pulp

abnormal T cell physiology ( J:140671 )

• splenic T cells have reduced migratory responses to CXCL12, CCL21, and CCL19

decreased T cell proliferation ( J:140671 )

• T cells activated in vitro have significantly less proliferation than controls

increased circulating interleukin-5 level ( J:140671 )

• the average serum concentrations of IL-5 (17 pg/ml vs 4.5 pg/ml in WT) are significantly increased

abnormal mesenteric lymph node morphology ( J:140671 )

• while reduced B cells are found throughout the rest of the body, mesenteric lymph nodes have 2- to 3- fold increased numbers of B cells

abnormal lymph node B cell domain morphology ( J:140671 )

• there are increases in the size and cellular densities of B cell follicles within the mesenteric lymph nodes

endocrine/exocrine glands

abnormal thymus medulla morphology ( J:140671 )

• mature CD4⁺ T cells accumulate in the medulla of the thymus

testis hypoplasia ( J:140671 )

♂ • testes showed drastic testicular degeneration

hematopoietic system

enhanced B cell migration ( J:140671 )

• splenic B cells have a slightly enhanced migratory response to the chemokine CXCL12

increased B cell proliferation ( J:140671 )

• B cells activated in vitro with LPS have significantly higher proliferation rates

abnormal thymus medulla morphology ( J:140671 )

• mature CD4⁺ T cells accumulate in the medulla of the thymus

abnormal leukocyte cell number ( J:140671 )

decreased immature B cell number ( J:140671 )

• immature B cells numbers are reduced by almost half in the bone marrow of adult mice

• newly formed B cells are reduced by 20-30% in the spleen of adult mice

decreased transitional stage B cell number ( J:140671 )

• T1 and T2 transitional B cells are reduced by 20-30% in the spleen of adult mice

increased eosinophil cell number ( J:140671 )

• the proportion of eosinophils in the bone marrow is three times higher than in controls

• increased numbers of eosinophils are also observed in the lung

• these increase numbers correlate with an increased amount of circulating IL-5

• these eosinophil differences are more clearly observed between the 12- to 55-wk-old groups than between the 6- and 11-wk-old groups

decreased mature B cell number ( J:140671 )

• mature B cell numbers are reduced by more than half in the bone marrow of adult mice

decreased follicular B cell number ( J:140671 )

• follicular B cells are reduced by 20-30% in the spleen of adult mice

decreased marginal zone B cell number ( J:140671 )

• MZ B cells are virtually absent in the spleen of adult mice

decreased pre-B cell number ( J:140671 )

• pre-B cell numbers are reduced in the bone marrow to varying degrees depending on age

• numbers are reduced by about 25% at 7 weeks of age and by over 50% at 12-32 weeks of age

decreased T cell number ( J:140671 )

• there is a 80-90% reduction in the number of circulating T cells found in adults

decreased double-positive T cell number ( J:140671 )

• the percentage of double-positive thymocytes is reduced by 30% compared to controls at 18 weeks of age

increased single-positive T cell number ( J:140671 )

• the percentages of single-positive thymocytes are increased 2-fold

spleen hypoplasia ( J:140671 )

• spleen cellularity is reduced in these mice

disorganized spleen B cell follicle ( J:140671 )

• localization of B cells is disorganized in the border region of the white pulp

abnormal T cell physiology ( J:140671 )

• splenic T cells have reduced migratory responses to CXCL12, CCL21, and CCL19

decreased T cell proliferation ( J:140671 )

• T cells activated in vitro have significantly less proliferation than controls

homeostasis/metabolism

increased circulating interleukin-5 level ( J:140671 )

• the average serum concentrations of IL-5 (17 pg/ml vs 4.5 pg/ml in WT) are significantly increased

reproductive system

testis hypoplasia ( J:140671 )

♂ • testes showed drastic testicular degeneration

male infertility ( J:140671 )

♂ • male mice are totally sterile

cellular

enhanced B cell migration ( J:140671 )

• splenic B cells have a slightly enhanced migratory response to the chemokine CXCL12

increased B cell proliferation ( J:140671 )

• B cells activated in vitro with LPS have significantly higher proliferation rates

decreased T cell proliferation ( J:140671 )

• T cells activated in vitro have significantly less proliferation than controls