mortality/aging
|
N |
• the early embryonic lethality associated with Pcnatm1.1Mdsc homozygotes is rescued by the presence of the transgene
|
reproductive system
|
|
• most meiotic chromosomes in mutant mice display elongated chromosome axes
|
 |
• testis are 40% the size of wild-type testis
|
|
|
• meiotic progression is disrupted at early pachynema resulting in a complete loss of spermatocytes
|
immune system
|
|
• there is a 50% reduction of IgG3 surface expression compared to wild-type on B cells stimulated ex vivo with LPS to induce switching from IgM to IgG3
• there is a 25% reduction of surface IgG1 expression compared to wild-type on B cells stimulated ex vivo with LPS and IL-4 to induce switching to IgG1
• the decrease in switching to IgG1 accumulated after the second division, indicating a decrease in switching at each generation
• switch junctions between mu and gamma3 segments contain less mutations than in wild-type B cells
|
|
|
• splenic B cells had a higher rate of somatic mutations in the rearranged V186.2 gene segment after immunization with NP antigen
• the overall frequency of unique mutations at the V186.2 gene segment was 9.1 x 10-2 mutations/base, which was significantly higher than the 7.2 x 10-2 mutations/base detected in wild-type mice
• mutations that occur at A:T site is reduced by about 2-fold while the frequency of C:G mutations is increased by 2-fold
• there is also an increase in mutations of GYW motifs that are considered a sight of action for activation-induced cytidine deaminase
|
hematopoietic system
|
|
• there is a 50% reduction of IgG3 surface expression compared to wild-type on B cells stimulated ex vivo with LPS to induce switching from IgM to IgG3
• there is a 25% reduction of surface IgG1 expression compared to wild-type on B cells stimulated ex vivo with LPS and IL-4 to induce switching to IgG1
• the decrease in switching to IgG1 accumulated after the second division, indicating a decrease in switching at each generation
• switch junctions between mu and gamma3 segments contain less mutations than in wild-type B cells
|
|
|
• splenic B cells had a higher rate of somatic mutations in the rearranged V186.2 gene segment after immunization with NP antigen
• the overall frequency of unique mutations at the V186.2 gene segment was 9.1 x 10-2 mutations/base, which was significantly higher than the 7.2 x 10-2 mutations/base detected in wild-type mice
• mutations that occur at A:T site is reduced by about 2-fold while the frequency of C:G mutations is increased by 2-fold
• there is also an increase in mutations of GYW motifs that are considered a sight of action for activation-induced cytidine deaminase
|
endocrine/exocrine glands
|
|
• testis are 40% the size of wild-type testis
|
cellular
|
|
• most meiotic chromosomes in mutant mice display elongated chromosome axes
|
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