Phenotypes associated with this allele

• Allele Symbol: Sting1^tm1Gnb
• Allele Name: targeted mutation 1, Glen N Barber
• Allele ID: MGI:3817418
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sting1^tm1Gnb/Sting1^tm1Gnb	involves: 129P2/OlaHsd	MGI:4939599
hm2	Sting1^tm1Gnb/Sting1^tm1Gnb	involves: 129P2/OlaHsd * C57BL/6	MGI:3817420
cx3	Rnaseh2a^tm1Crh/Rnaseh2a^tm1Crh Sting1^tm1Gnb/Sting1^tm1Gnb	involves: 129P2/OlaHsd	MGI:6274938
cx4	Sting1^tm1Gnb/Sting1^tm1Gnb Trex1^tm1Tld/Trex1^tm1Tld	involves: 129P2/OlaHsd * C57BL/6	MGI:5317330

Genotype
MGI:4939599

hm1

• Allelic Composition: Sting1^tm1Gnb/Sting1^tm1Gnb
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal response to infection ( J:168746 )

• macrophages exhibit a modest decrease in response to Sendai virus compared with wild-type cells

Genotype
MGI:3817420

hm2

• Allelic Composition: Sting1^tm1Gnb/Sting1^tm1Gnb
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal innate immunity ( J:141011 )

• mouse embryonic fibroblasts (MEF) are defective in initiating immune defenses (i.e. interferon-beta induction ) in response to negative strand viruses such as vesicular stomatitis virus (VSV) and the Sendai virus or the DNA herpes simplex virus 1

• b-form DNA, interferon stimulatory DNA, and the bacteria Listeria monocytogenes also fail to elicit a response in mutant MEFs

abnormal susceptibility to Herpesvirales infection ( J:141011 )

• MEFs produce less interferon-beta in response to herpes simplex-1 virus

increased susceptibility to Riboviria infection ( J:141011 )

• MEFs infected with vesicular stomatitis virus (VSV) have 100-fold higher titer number than controls 24 to 36 hours after infection

• MEFs produce less interferon-beta in response to VSV and the Sendai viruses

• less susceptibility to VSV is observed in bone marrow-derived dendritic cells or macrophages

Genotype
MGI:6274938

cx3

• Allelic Composition: Rnaseh2a^tm1Crh/Rnaseh2a^tm1Crh; Sting1^tm1Gnb/Sting1^tm1Gnb
• Genetic Background: involves: 129P2/OlaHsd

mortality/aging

perinatal lethality, incomplete penetrance ( J:232933 )

• mice are either perinatal lethal or survive after birth; survival is at 6% of expected frequency with 2% of pups surviving weaning

growth/size/body

decreased body size ( J:232933 )

• surviving mice are about 70% in body size and weight compared to controls

decreased body weight ( J:232933 )

• surviving mice are about 70% in body size and weight compared to controls

pigmentation

abnormal hair shaft melanin granule morphology ( J:232933 )

• white patches show lack of melanin in hair shafts

• however, skin histology from white patches in normal

white spotting ( J:232933 )

• all viable mice present a ventral white spotting phenotype and white hind- and forepaws from birth which remains through life

cellular

abnormal cell physiology ( J:232933 )

• E13.5 and E15.5 embryos exhibit increased LINE-1 retroelement DNA levels in cytosolic extract, however, no elevation in L1 ORF1 protein is seen

integument

abnormal hair shaft melanin granule morphology ( J:232933 )

• white patches show lack of melanin in hair shafts

• however, skin histology from white patches in normal

white spotting ( J:232933 )

• all viable mice present a ventral white spotting phenotype and white hind- and forepaws from birth which remains through life

reproductive system

infertility ( J:232933 )

• mice fail to produce offspring

immune system

immune system phenotype ( J:232933 )

N • no abnormalities or inflammation in internal organs, including the brain, are seen in viable mice and expression of interferon-stimulated genes is restored to normal levels

Genotype
MGI:5317330

cx4

• Allelic Composition: Sting1^tm1Gnb/Sting1^tm1Gnb; Trex1^tm1Tld/Trex1^tm1Tld
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

mortality/aging ( J:181257 )

N • mice exhibit normal survival unlike Trex1^tm1Tld homozygotes

immune system

immune system phenotype ( J:181257 )

N • mice do not exhibit multi-organ inflammation unlike Trex1^tm1Tld homozygotes