Phenotypes associated with this allele

• Allele Symbol: Tg(CD2-Zbtb7b)C8Rbo
• Allele Name: transgene insertion C8, Remy Bosselut
• Allele ID: MGI:3817775
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(CD2-Zbtb7b)C8Rbo/? Themis^tm1Lov/Themis^tm1Lov	involves: 129	MGI:4353392
cx2	Tg(CD2-Zbtb7b)C8Rbo/? Tg(TcraH-Y,TcrbH-Y)71Vbo/?	involves: C57BL/6 * DBA/2J	MGI:3817861
cx3	Tg(CD2-Zbtb7b)C8Rbo/? Tg(TcrLCMV)327Sdz/?	involves: C57BL/6 * DBA/2J	MGI:3817863
tg4	Tg(CD2-Zbtb7b)C8Rbo/?	involves: C57BL/6	MGI:3817859

Genotype
MGI:4353392

cx1

• Allelic Composition: Tg(CD2-Zbtb7b)C8Rbo/?; Themis^tm1Lov/Themis^tm1Lov
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T cell differentiation ( J:151075 )

• CD8 single positive thymocytes fail to develop in these mice

• transgene expression fails to alleviate the partial block in CD4 development

hematopoietic system

abnormal T cell differentiation ( J:151075 )

• CD8 single positive thymocytes fail to develop in these mice

• transgene expression fails to alleviate the partial block in CD4 development

Genotype
MGI:3817861

cx2

• Allelic Composition: Tg(CD2-Zbtb7b)C8Rbo/?; Tg(TcraH-Y,TcrbH-Y)71Vbo/?
• Genetic Background: involves: C57BL/6 * DBA/2J

immune system

abnormal T cell differentiation ( J:97610 )

• female mice have substantial numbers of HY-specific CD4⁺ T cells while having a complete absence of HY-specific CD8⁺ T cells, which is a reversal of the T cell populations that normally develop in female mice carrying just the Tg(TcraH-Y,TcrbH-Y)71Vbo allele

hematopoietic system

abnormal T cell differentiation ( J:97610 )

• female mice have substantial numbers of HY-specific CD4⁺ T cells while having a complete absence of HY-specific CD8⁺ T cells, which is a reversal of the T cell populations that normally develop in female mice carrying just the Tg(TcraH-Y,TcrbH-Y)71Vbo allele

Genotype
MGI:3817863

cx3

• Allelic Composition: Tg(CD2-Zbtb7b)C8Rbo/?; Tg(TcrLCMV)327Sdz/?
• Genetic Background: involves: C57BL/6 * DBA/2J

immune system

abnormal T cell differentiation ( J:97610 )

• mice have substantial numbers of transgenic CD4⁺ T cells while having a complete absence of transgenic CD8⁺ T cells, which is a reversal of the T cell populations that normally develop in mice carrying just the Tg(TcrLCMV)327Sdz allele

• the CD4 T cells bear high levels of the transgenic TCR, express CD154 upon activation, and have other hallmarks (lack perforin and IFN-gamma expression) of truly belonging to the CD4 lineage and not the CD8 lineage

hematopoietic system

abnormal T cell differentiation ( J:97610 )

• mice have substantial numbers of transgenic CD4⁺ T cells while having a complete absence of transgenic CD8⁺ T cells, which is a reversal of the T cell populations that normally develop in mice carrying just the Tg(TcrLCMV)327Sdz allele

• the CD4 T cells bear high levels of the transgenic TCR, express CD154 upon activation, and have other hallmarks (lack perforin and IFN-gamma expression) of truly belonging to the CD4 lineage and not the CD8 lineage

Genotype
MGI:3817859

tg4

• Allelic Composition: Tg(CD2-Zbtb7b)C8Rbo/?
• Genetic Background: involves: C57BL/6

immune system

increased CD4-positive, alpha-beta T cell number ( J:97610 )

• CD4 T cell numbers are almost doubled in the spleen

absent CD8-positive, alpha-beta T cells ( J:97610 )

• CD8 T cells are virtually absent in the periphery (less than 1% of controls)

• single positive CD8⁺ TCR^hi thymocytes are also less than 1% of controls

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:97610 )

• CD4 T cell numbers are almost doubled in the spleen

absent CD8-positive, alpha-beta T cells ( J:97610 )

• CD8 T cells are virtually absent in the periphery (less than 1% of controls)

• single positive CD8⁺ TCR^hi thymocytes are also less than 1% of controls