Analysis Tools
cardiovascular system
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• mutants show no vascular defects during normal development or during oxygen-induced retinopathy
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• aortic ring explants embedded in Matrigel show impaired microvascular sprouting
• matrigel plugs that were excised from mutants 7 days after implantation show diminished vascularization with a 60% decrease in vascular area and hemoglobin content compared to excised plugs from wild-type
• in a corneal micropocket assay, mutants show minimal bFGF-stimulated vessel spouting in the corneas compared to wild-type
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• lung endothelial cell spreading on Matrigel, chemotaxis toward bFGF, and invasion through Matrigel are decreased
• intrinsic 2-dimensional migration of lung endothelial cells is increased for cells plated on Matrigel, and to a lesser extent on gelatin
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• formation of cellular cables by mutant lung endothelial cells is markedly diminished
• Matrigel contraction by lung endothelial cells is impaired by 60% compared to wild-type
• lung endothelial cells grown within a type I collagen gel show a 48% decrease in tube formation
• spheroids from lung endothelial cells grown in collagen gen containing bFGF and VEGF exhibit a decrease in the number of sprouts from each spheroid and the length of the capillary-like sprouts from a spheroid is significantly shorter than in wild-type
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neoplasm
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• mutants implanted with LLC cells develop smaller solid tumors, show a reduced tumor volume and weight, and tumors show decreased microvessel density
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cellular
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• lung endothelial cell spreading on Matrigel, chemotaxis toward bFGF, and invasion through Matrigel are decreased
• intrinsic 2-dimensional migration of lung endothelial cells is increased for cells plated on Matrigel, and to a lesser extent on gelatin
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