Phenotypes associated with this allele

• Allele Symbol: Nfatc3^tm1Grc
• Allele Name: targeted mutation 1, Gerald R Crabtree
• Allele ID: MGI:3820410
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nfatc3^tm1Grc/Nfatc3^tm1Grc	Not Specified	MGI:3820652
cn2	Nfatc3^tm1Grc/Nfatc3^tm1Grc Tg(Lck-cre)I57Jxm/0	involves: ICR	MGI:3820650
cn3	Nfatc2^tm1Rao/Nfatc2^tm1Rao Nfatc3^tm1Grc/Nfatc3^tm1Grc Tg(Lck-cre)I57Jxm/0	involves: ICR	MGI:3820653

Genotype
MGI:3820652

hm1

• Allelic Composition: Nfatc3^tm1Grc/Nfatc3^tm1Grc
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:141917 )

• mice are indistinguishable from wild-type mice

Genotype
MGI:3820650

cn2

• Allelic Composition: Nfatc3^tm1Grc/Nfatc3^tm1Grc; Tg(Lck-cre)I57Jxm/0
• Genetic Background: involves: ICR

immune system

increased T cell apoptosis ( J:141917 )

• in culture, the initial number of double positive T cell undergoing apoptosis is greater than for wild-type cells

• however, the rate of apoptosis in vitro is normal

thymus hypoplasia ( J:141917 )

• the total number of thymocytes is reduced by half of wild-type

decreased thymocyte number ( J:141917 )

• the total number of thymocytes is reduced by half of wild-type

• the number of single positive T cells in the thymus is lower than in wild-type mice

• however, the number of double positive cells is normal

abnormal T cell differentiation ( J:141917 )

• T cell development is partially blocked after pre-TCR signaling and the DN4 population is reduced compared to in wild-type mice

increased double-negative T cell number ( J:141917 )

• mice exhibit an increase in double negative T cell compared to in wild-type mice up to stage DN4

decreased CD4-positive, alpha-beta T cell number ( J:141917 )

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:141917 )

• in the thymus

lymph node hypoplasia ( J:141917 )

• the total number of inguinal lymph node cells is decreased by half of wild-type

hematopoietic system

increased T cell apoptosis ( J:141917 )

• in culture, the initial number of double positive T cell undergoing apoptosis is greater than for wild-type cells

• however, the rate of apoptosis in vitro is normal

thymus hypoplasia ( J:141917 )

• the total number of thymocytes is reduced by half of wild-type

decreased thymocyte number ( J:141917 )

• the total number of thymocytes is reduced by half of wild-type

• the number of single positive T cells in the thymus is lower than in wild-type mice

• however, the number of double positive cells is normal

abnormal T cell differentiation ( J:141917 )

• T cell development is partially blocked after pre-TCR signaling and the DN4 population is reduced compared to in wild-type mice

increased double-negative T cell number ( J:141917 )

• mice exhibit an increase in double negative T cell compared to in wild-type mice up to stage DN4

decreased CD4-positive, alpha-beta T cell number ( J:141917 )

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:141917 )

• in the thymus

cellular

increased T cell apoptosis ( J:141917 )

• in culture, the initial number of double positive T cell undergoing apoptosis is greater than for wild-type cells

• however, the rate of apoptosis in vitro is normal

endocrine/exocrine glands

thymus hypoplasia ( J:141917 )

• the total number of thymocytes is reduced by half of wild-type

decreased thymocyte number ( J:141917 )

• the total number of thymocytes is reduced by half of wild-type

• the number of single positive T cells in the thymus is lower than in wild-type mice

• however, the number of double positive cells is normal

Genotype
MGI:3820653

cn3

• Allelic Composition: Nfatc2^tm1Rao/Nfatc2^tm1Rao; Nfatc3^tm1Grc/Nfatc3^tm1Grc; Tg(Lck-cre)I57Jxm/0
• Genetic Background: involves: ICR

immune system

increased T cell apoptosis ( J:141917 )

• the rate of T cells undergoing apoptosis in culture is slightly greater than in wild-type and Nfatc3^tm1Grc/Nfatc3^tm1Grc Tg(Lck-cre)I57Jxm mice

• however, the number of T cells initially undergoing apoptosis is normal

abnormal T cell differentiation ( J:141917 )

• T cell development is partially blocked after pre-TCR signaling and the DN4 population is reduced compared to in wild-type mice but is not more severe than in Nfatc3^tm1Grc/Nfatc3^tm1Grc Tg(Lck-cre)I57Jxm mice

abnormal double-negative T cell morphology ( J:141917 )

• mice exhibit an increase in DN1 and subsequently a more severe decrease in the number of DN4 T cells compared to in wild-type, Nfactc3 null or Nfact2 null mice

hematopoietic system

increased T cell apoptosis ( J:141917 )

• the rate of T cells undergoing apoptosis in culture is slightly greater than in wild-type and Nfatc3^tm1Grc/Nfatc3^tm1Grc Tg(Lck-cre)I57Jxm mice

• however, the number of T cells initially undergoing apoptosis is normal

abnormal T cell differentiation ( J:141917 )

• T cell development is partially blocked after pre-TCR signaling and the DN4 population is reduced compared to in wild-type mice but is not more severe than in Nfatc3^tm1Grc/Nfatc3^tm1Grc Tg(Lck-cre)I57Jxm mice

abnormal double-negative T cell morphology ( J:141917 )

• mice exhibit an increase in DN1 and subsequently a more severe decrease in the number of DN4 T cells compared to in wild-type, Nfactc3 null or Nfact2 null mice

cellular

increased T cell apoptosis ( J:141917 )

• the rate of T cells undergoing apoptosis in culture is slightly greater than in wild-type and Nfatc3^tm1Grc/Nfatc3^tm1Grc Tg(Lck-cre)I57Jxm mice

• however, the number of T cells initially undergoing apoptosis is normal