Phenotypes associated with this allele

• Allele Symbol: Flrt3^tm2Kln
• Allele Name: targeted mutation 2, Rudiger Klein
• Allele ID: MGI:3821877
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Flrt3^tm2Kln/Flrt3^tm2Kln	involves: 129P2/OlaHsd * 129S/SvEv	MGI:3821882
hm2	Flrt3^tm2Kln/Flrt3^tm2Kln	involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6	MGI:3821880
hm3	Flrt3^tm2Kln/Flrt3^tm2Kln	involves: 129P2/OlaHsd * 129S/SvEv * CD-1	MGI:3821881
ht4	Flrt3^tm1Kln/Flrt3^tm2Kln	involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6	MGI:3821879
cn5	Flrt3^tm2Kln/Flrt3^tm3Kln Tg(Pgk1-cre)1Lni/0	involves: 129P2/OlaHsd * 129S/SvEv * BALB/c * C57BL/6	MGI:5495542

Genotype
MGI:3821882

hm1

• Allelic Composition: Flrt3^tm2Kln/Flrt3^tm2Kln
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:142039 )

• by P15 less than 3% of mice are viable

Genotype
MGI:3821880

hm2

• Allelic Composition: Flrt3^tm2Kln/Flrt3^tm2Kln
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6

mortality/aging

postnatal lethality, incomplete penetrance ( J:142039 )

• by P15 less than 3% of mice are viable

embryonic lethality during organogenesis, incomplete penetrance ( J:142039 )

• fewer than expected mice are present at E12.5 to E13.5

embryo

abnormal embryo development ( J:142039 )

• at E8.5 to E9.5, some embryos develop outside of the yolk sac

• during head fold development, the neuroectoderm replaces the definitive ectoderm and the anterior portion of the embryo breaks through the yolk sac unlike in wild-type mice

• however, embryonic patterning and cell proliferation, apoptosis and migration during early development are normal

failure of initiation of embryo turning ( J:142039 )

• in some mice at E8.5 to E9.5

embryonic growth retardation ( J:142039 )

abnormal neural tube morphology ( J:142039 )

• at E8.5 to E9.5, some mice exhibit asymmetric head folds while others exhibit heads that develop outside of the yolk sac

incomplete rostral neuropore closure ( J:142039 )

• some mice exhibit an anterior neural tube closure defect

disorganized embryonic tissue ( J:142039 )

• in some mice at E8.5 to E9.5

abnormal anterior visceral endoderm morphology ( J:142039 )

• 79% of mice exhibit a rupture in the basement membrane that results in holes in the anterior visceral endoderm

• disruption of the basement membrane precedes disruption in the anterior visceral endoderm and induces epithelial-mesenchymal transition unlike in wild-type mice

nervous system

abnormal neural tube morphology ( J:142039 )

• at E8.5 to E9.5, some mice exhibit asymmetric head folds while others exhibit heads that develop outside of the yolk sac

incomplete rostral neuropore closure ( J:142039 )

• some mice exhibit an anterior neural tube closure defect

cardiovascular system

cardia bifida ( J:142039 )

• in some mice at E8.5 to E9.5

growth/size/body

embryonic growth retardation ( J:142039 )

failure of ventral body wall closure ( J:142039 )

• some mice exhibit failure of the ventral body to close

cellular

abnormal basement membrane morphology ( J:142039 )

• 79% of mice exhibit a rupture in the basement membrane that results in holes in the anterior visceral endoderm

• disruption of the basement membrane precedes disruption in the anterior visceral endoderm and induces epithelial-mesenchymal transition unlike in wild-type mice

Genotype
MGI:3821881

hm3

• Allelic Composition: Flrt3^tm2Kln/Flrt3^tm2Kln
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * CD-1

mortality/aging

postnatal lethality, incomplete penetrance ( J:142039 )

• by P15 less than 3% of mice are viable

Genotype
MGI:3821879

ht4

• Allelic Composition: Flrt3^tm1Kln/Flrt3^tm2Kln
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6

embryo

abnormal anterior visceral endoderm morphology ( J:142039 )

• 19 of 24 mice exhibit a rupture in the basement membrane that results in holes in the anterior visceral endoderm

cellular

abnormal basement membrane morphology ( J:142039 )

• 19 of 24 mice exhibit a rupture in the basement membrane that results in holes in the anterior visceral endoderm

Genotype
MGI:5495542

cn5

• Allelic Composition: Flrt3^tm2Kln/Flrt3^tm3Kln; Tg(Pgk1-cre)1Lni/0
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * BALB/c * C57BL/6

embryo

abnormal neural tube closure ( J:196042 )

• open head

nervous system

abnormal neural tube closure ( J:196042 )

• open head