Phenotypes associated with this allele

• Allele Symbol: Igh^tm3Hws
• Allele Name: targeted mutation 3, Harry W Schroeder
• Allele ID: MGI:3822178
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Igh^tm3Hws/Igh^tm3Hws	BALB/c-Igh^tm3Hws	MGI:3822179
hm2	Igh^tm3Hws/Igh^tm3Hws	involves: BALB/c	MGI:3822283

Genotype
MGI:3822179

hm1

• Allelic Composition: Igh^tm3Hws/Igh^tm3Hws
• Genetic Background: BALB/c-Igh^tm3Hws

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal immunoglobulin heavy chain V(D)J recombination ( J:142068 )

• the ratio of reading frame-1 usage of the DFL16.1 gene segment to reading frame-2 usage is reversed in the B cells of these mice

• mature B cells express heavy-chains with increased hydrophobicity compared to controls

• unique in-frame heavy chain transcripts from B cells at all stages of development have greater than 7 nucleotides lost at the 3' end

• unique in-frame heavy chain transcripts from mature B cells have decreased 5' nucleotide loss

hematopoietic system

abnormal immunoglobulin heavy chain V(D)J recombination ( J:142068 )

• the ratio of reading frame-1 usage of the DFL16.1 gene segment to reading frame-2 usage is reversed in the B cells of these mice

• mature B cells express heavy-chains with increased hydrophobicity compared to controls

• unique in-frame heavy chain transcripts from B cells at all stages of development have greater than 7 nucleotides lost at the 3' end

• unique in-frame heavy chain transcripts from mature B cells have decreased 5' nucleotide loss

Genotype
MGI:3822283

hm2

• Allelic Composition: Igh^tm3Hws/Igh^tm3Hws
• Genetic Background: involves: BALB/c

immune system

decreased late pro-B cell number ( J:142069 )

• there is over a 40% decrease in the number of late pro-B cells (i.e. fraction D cells) compared to controls

decreased B cell number ( J:142069 )

• numbers of CD19⁺ cells are reduced in the bone marrow and spleen by around 20% to 30%

decreased immature B cell number ( J:142069 )

• there is about a 40% decrease in the number of immature B cells (ie. fraction E cells) found in the bone marrow

decreased transitional stage B cell number ( J:142069 )

• there is about a 30% decrease in the number of transitional T3(A) B cells (i.e. "anergic" B cells) found in the spleen

decreased mature B cell number ( J:142069 )

• there is about a 40% decrease in the number of mature recirculating B cells found in the bone marrow

decreased follicular B cell number ( J:142069 )

• there is about a 40% decrease in the number of follicular B cells found in the spleen

spleen hypoplasia ( J:142069 )

• spleen cellularity is reduced around 20% compared to controls

abnormal immunoglobulin level ( J:142069 )

decreased IgG1 level ( J:142069 )

• antigen specific IgG1 levels are reduced 6-fold compared to wild-type in response to immunization with a recombinant strain of Salmonella that elicits a T cell-dependent B cell response

decreased IgG2a level ( J:142069 )

• antigen specific IgG2a levels are reduced 6-fold compared to wild-type in response to immunization with a recombinant strain of Salmonella that elicits a T cell-dependent B cell response

decreased IgG2b level ( J:142069 )

• antigen specific IgG2a levels are reduced 34-fold compared to wild-type in response to immunization with a recombinant strain of Salmonella that elicits a T cell-dependent B cell response

decreased IgM level ( J:142069 )

• antigen specific IgM levels are one-third of that observed in wild-type controls seven days after immunization with the T cell-independent antigen DEX

increased IgM level ( J:142069 )

• antigen specific IgM levels increase 4-fold over wild-type in response to immunization with a recombinant strain of Salmonella that elicits a T cell-dependent B cell response

hematopoietic system

decreased late pro-B cell number ( J:142069 )

• there is over a 40% decrease in the number of late pro-B cells (i.e. fraction D cells) compared to controls

decreased bone marrow cell number ( J:142069 )

• there is about a 25% reduction in the number of cells found in the bone marrow

decreased B cell number ( J:142069 )

• numbers of CD19⁺ cells are reduced in the bone marrow and spleen by around 20% to 30%

decreased immature B cell number ( J:142069 )

• there is about a 40% decrease in the number of immature B cells (ie. fraction E cells) found in the bone marrow

decreased transitional stage B cell number ( J:142069 )

• there is about a 30% decrease in the number of transitional T3(A) B cells (i.e. "anergic" B cells) found in the spleen

decreased mature B cell number ( J:142069 )

• there is about a 40% decrease in the number of mature recirculating B cells found in the bone marrow

decreased follicular B cell number ( J:142069 )

• there is about a 40% decrease in the number of follicular B cells found in the spleen

spleen hypoplasia ( J:142069 )

• spleen cellularity is reduced around 20% compared to controls

abnormal immunoglobulin level ( J:142069 )

decreased IgG1 level ( J:142069 )

• antigen specific IgG1 levels are reduced 6-fold compared to wild-type in response to immunization with a recombinant strain of Salmonella that elicits a T cell-dependent B cell response

decreased IgG2a level ( J:142069 )

• antigen specific IgG2a levels are reduced 6-fold compared to wild-type in response to immunization with a recombinant strain of Salmonella that elicits a T cell-dependent B cell response

decreased IgG2b level ( J:142069 )

• antigen specific IgG2a levels are reduced 34-fold compared to wild-type in response to immunization with a recombinant strain of Salmonella that elicits a T cell-dependent B cell response

decreased IgM level ( J:142069 )

• antigen specific IgM levels are one-third of that observed in wild-type controls seven days after immunization with the T cell-independent antigen DEX

increased IgM level ( J:142069 )

• antigen specific IgM levels increase 4-fold over wild-type in response to immunization with a recombinant strain of Salmonella that elicits a T cell-dependent B cell response