Phenotypes associated with this allele

• Allele Symbol: Nfkb1^tm1Sley
• Allele Name: targeted mutation 1, Steven C Ley
• Allele ID: MGI:3822895
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nfkb1^tm1Sley/Nfkb1^tm1Sley	involves: 129S4/SvJae	MGI:5491302
hm2	Nfkb1^tm1Sley/Nfkb1^tm1Sley	involves: 129S4/SvJae * 129S8/SvEv	MGI:3822909
hm3	Nfkb1^tm1Sley/Nfkb1^tm1Sley	involves: 129S4/SvJae * 129S8/SvEv * C57BL/6	MGI:3822910
cx4	Map3k8^tm1Pnt/Map3k8^tm1Pnt Nfkb1^tm1Sley/Nfkb1^tm1Sley	involves: 129S1/Sv * 129S4/SvJae	MGI:5491301
cx5	Map3k8^tm1Sley/Map3k8^tm1Sley Nfkb1^tm1Sley/Nfkb1^tm1Sley	involves: 129S4/SvJae	MGI:5491300

Genotype
MGI:5491302

hm1

• Allelic Composition: Nfkb1^tm1Sley/Nfkb1^tm1Sley
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased tumor necrosis factor secretion ( J:196528 )

• from bone marrow-derived macrophage stimulated with LPS

Genotype
MGI:3822909

hm2

• Allelic Composition: Nfkb1^tm1Sley/Nfkb1^tm1Sley
• Genetic Background: involves: 129S4/SvJae * 129S8/SvEv

immune system

decreased T cell proliferation ( J:142340 )

• nave CD4⁺ T cells stimulated in vitro through the TCR only have a third the proliferative response as control mice

• 66% fewer naive CD4⁺ T cells go into S-phase with 24 hours of TCR stimulation compared to controls

• the S-phase entry defect can be partly overcome by CD28 stimulation

• allogeneic proliferative response of nave CD4⁺ T cells is reduced 6-fold compared to controls

• the amount of IL-2 required for a half maximal proliferation response is 100-fold higher than in controls

decreased memory T cell number ( J:142340 )

• CD4⁺ memory T cells are reduced by 3-fold in the spleen

• splenic CD8⁺ memory T cells are also reduced

• reconstitution of wild-type mice with both wild-type and mutant bone marrow demonstrate an intrinsic defect of mutant hematopoietic cells to produce CD4⁺ memory T cells

decreased regulatory T cell number ( J:142340 )

• there is a 75% decrease in the number of regulatory T cells found in the spleen, lymph node and thymus of mice

• reconstitution of wild-type mice with both wild-type and mutant bone marrow demonstrate an intrinsic defect of mutant hematopoietic cells to produce CD4⁺ regulatory T cells

decreased circulating interleukin-2 level ( J:142340 )

• nave CD4⁺ T cells only produce one-third the amount of IL-2 as controls after in vitro stimulation

abnormal inguinal lymph node morphology ( J:142340 )

• most mice have small or absent inguinal lymph nodes

absent inguinal lymph nodes ( J:142340 )

small inguinal lymph nodes ( J:142340 )

hematopoietic system

decreased T cell proliferation ( J:142340 )

• nave CD4⁺ T cells stimulated in vitro through the TCR only have a third the proliferative response as control mice

• 66% fewer naive CD4⁺ T cells go into S-phase with 24 hours of TCR stimulation compared to controls

• the S-phase entry defect can be partly overcome by CD28 stimulation

• allogeneic proliferative response of nave CD4⁺ T cells is reduced 6-fold compared to controls

• the amount of IL-2 required for a half maximal proliferation response is 100-fold higher than in controls

decreased memory T cell number ( J:142340 )

• CD4⁺ memory T cells are reduced by 3-fold in the spleen

• splenic CD8⁺ memory T cells are also reduced

• reconstitution of wild-type mice with both wild-type and mutant bone marrow demonstrate an intrinsic defect of mutant hematopoietic cells to produce CD4⁺ memory T cells

decreased regulatory T cell number ( J:142340 )

• there is a 75% decrease in the number of regulatory T cells found in the spleen, lymph node and thymus of mice

• reconstitution of wild-type mice with both wild-type and mutant bone marrow demonstrate an intrinsic defect of mutant hematopoietic cells to produce CD4⁺ regulatory T cells

homeostasis/metabolism

decreased circulating interleukin-2 level ( J:142340 )

• nave CD4⁺ T cells only produce one-third the amount of IL-2 as controls after in vitro stimulation

cellular

decreased T cell proliferation ( J:142340 )

• nave CD4⁺ T cells stimulated in vitro through the TCR only have a third the proliferative response as control mice

• 66% fewer naive CD4⁺ T cells go into S-phase with 24 hours of TCR stimulation compared to controls

• the S-phase entry defect can be partly overcome by CD28 stimulation

• allogeneic proliferative response of nave CD4⁺ T cells is reduced 6-fold compared to controls

• the amount of IL-2 required for a half maximal proliferation response is 100-fold higher than in controls

Genotype
MGI:3822910

hm3

• Allelic Composition: Nfkb1^tm1Sley/Nfkb1^tm1Sley
• Genetic Background: involves: 129S4/SvJae * 129S8/SvEv * C57BL/6

immune system

decreased NK T cell number ( J:142340 )

• there are slightly fewer thymic TCRbeta⁺NK1.1⁺ NKT cells

• the number of thymic CD1d-dependent NKT is also reduced

decreased memory T cell number ( J:142340 )

• reconstitution of wild-type mice with mutant bone marrow leads to lower CD4⁺ memory T cells in the spleen and lymph nodes compared to controls reconstituted with wild-type bone marrow

decreased regulatory T cell number ( J:142340 )

• reconstitution of wild-type mice with mutant bone marrow leads to 3-fold lower FoxP3⁺ CD4⁺ regulatory T cells in the spleen and lymph nodes compared to controls reconstituted with wild-type bone marrow

hematopoietic system

decreased NK T cell number ( J:142340 )

• there are slightly fewer thymic TCRbeta⁺NK1.1⁺ NKT cells

• the number of thymic CD1d-dependent NKT is also reduced

decreased memory T cell number ( J:142340 )

• reconstitution of wild-type mice with mutant bone marrow leads to lower CD4⁺ memory T cells in the spleen and lymph nodes compared to controls reconstituted with wild-type bone marrow

decreased regulatory T cell number ( J:142340 )

• reconstitution of wild-type mice with mutant bone marrow leads to 3-fold lower FoxP3⁺ CD4⁺ regulatory T cells in the spleen and lymph nodes compared to controls reconstituted with wild-type bone marrow

Genotype
MGI:5491301

cx4

• Allelic Composition: Map3k8^tm1Pnt/Map3k8^tm1Pnt; Nfkb1^tm1Sley/Nfkb1^tm1Sley
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae

immune system

decreased circulating interleukin-12b level ( J:196528 )

• after LPS injection

decreased interleukin-12b secretion ( J:196528 )

• in bone marrow-derived macrophage

decreased tumor necrosis factor secretion ( J:196528 )

• from bone marrow-derived macrophage stimulated with LPS

• from primary peritoneal macrophages from mice stimulated with LPS

homeostasis/metabolism

decreased circulating interleukin-12b level ( J:196528 )

• after LPS injection

Genotype
MGI:5491300

cx5

• Allelic Composition: Map3k8^tm1Sley/Map3k8^tm1Sley; Nfkb1^tm1Sley/Nfkb1^tm1Sley
• Genetic Background: involves: 129S4/SvJae

immune system

decreased tumor necrosis factor secretion ( J:196528 )

• from bone marrow-derived macrophage stimulated with LPS