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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Rhohtm1Brak
targeted mutation 1, Cord Brakebusch
MGI:3826431
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Rhohtm1Brak/Rhohtm1Brak involves: 129/Sv * C57BL/6 MGI:3826455
cx2
 		Rhohtm1Brak/Rhohtm1Brak
Itgb2tm2Bay/Itgb2tm2Bay 		involves: 129/Sv * C57BL/6 MGI:3826459
cx3
 		Rhohtm1Brak/Rhohtm1Brak
Tg(TcraTcrb)425Cbn/? 		involves: 129/Sv * C57BL/6 MGI:3826463


Genotype
MGI:3826455
hm1
Allelic
Composition		 Rhohtm1Brak/Rhohtm1Brak
Genetic
Background		 involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rhohtm1Brak mutation (0 available); any Rhoh mutation (13 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell apoptosis ( J:143161 )
• DN4 cells show increased apoptosis
• double and single positive T cells show increased apoptosis
decreased T cell proliferation ( J:143161 )
• DN3 and DN4 proliferation is significantly reduced
increased T cell proliferation ( J:143161 )
• proliferation of single positive T cells is increased
abnormal double-negative T cell morphology ( J:143161 )
• DN3 to DN4 transition is defective
increased NK cell number ( J:143161 )
• elevated in bone marrow and spleen at 2 months but not at 6 months
decreased T cell number ( J:143161 )
• T cell counts are greatly reduced in bone marrow, spleen, and lymph nodes at 2 months
decreased double-positive T cell number ( J:143161 )
• reduced 60% in thymus
decreased CD4-positive, alpha-beta T cell number ( J:143161 )
• reduced 50% in bone marrow and spleen
• reduced 80% in thymus
decreased CD8-positive, alpha-beta T cell number ( J:143161 )
• reduced 75% in bone marrow and spleen
• reduced 85% in thymus
increased double-negative T cell number ( J:143161 )
• increased 2 fold in the thymus
• DN3 cells in the thymus are increased 3 fold while DN4 cell numbers are essentially normal
• DN3 cells are slightly elevated in the spleen and significantly elevated in lymph nodes
increased NK T cell number ( J:143161 )
• elevated in bone marrow and spleen at 2 months but not at 6 months
increased gamma-delta T cell number ( J:143161 )
• slightly elevated in the spleen and significantly elevated in lymph nodes
thymus hypoplasia ( J:143161 )
• about 60% lower cell numbers than controls at 2 months
spleen hypoplasia ( J:143161 )
• splenocyte counts are reduced at 2 months of age
abnormal splenic cell ratio ( J:143161 )
• splenic monocyte counts are slightly elevated

hematopoietic system
decreased T cell apoptosis ( J:143161 )
• DN4 cells show increased apoptosis
• double and single positive T cells show increased apoptosis
decreased T cell proliferation ( J:143161 )
• DN3 and DN4 proliferation is significantly reduced
increased T cell proliferation ( J:143161 )
• proliferation of single positive T cells is increased
thymus hypoplasia ( J:143161 )
• about 60% lower cell numbers than controls at 2 months
abnormal double-negative T cell morphology ( J:143161 )
• DN3 to DN4 transition is defective
increased NK cell number ( J:143161 )
• elevated in bone marrow and spleen at 2 months but not at 6 months
decreased T cell number ( J:143161 )
• T cell counts are greatly reduced in bone marrow, spleen, and lymph nodes at 2 months
decreased double-positive T cell number ( J:143161 )
• reduced 60% in thymus
decreased CD4-positive, alpha-beta T cell number ( J:143161 )
• reduced 50% in bone marrow and spleen
• reduced 80% in thymus
decreased CD8-positive, alpha-beta T cell number ( J:143161 )
• reduced 75% in bone marrow and spleen
• reduced 85% in thymus
increased double-negative T cell number ( J:143161 )
• increased 2 fold in the thymus
• DN3 cells in the thymus are increased 3 fold while DN4 cell numbers are essentially normal
• DN3 cells are slightly elevated in the spleen and significantly elevated in lymph nodes
increased NK T cell number ( J:143161 )
• elevated in bone marrow and spleen at 2 months but not at 6 months
increased gamma-delta T cell number ( J:143161 )
• slightly elevated in the spleen and significantly elevated in lymph nodes
spleen hypoplasia ( J:143161 )
• splenocyte counts are reduced at 2 months of age
abnormal splenic cell ratio ( J:143161 )
• splenic monocyte counts are slightly elevated

cellular
decreased T cell apoptosis ( J:143161 )
• DN4 cells show increased apoptosis
• double and single positive T cells show increased apoptosis
decreased T cell proliferation ( J:143161 )
• DN3 and DN4 proliferation is significantly reduced
increased T cell proliferation ( J:143161 )
• proliferation of single positive T cells is increased

endocrine/exocrine glands
thymus hypoplasia ( J:143161 )
• about 60% lower cell numbers than controls at 2 months




Genotype
MGI:3826459
cx2
Allelic
Composition		 Rhohtm1Brak/Rhohtm1Brak
Itgb2tm2Bay/Itgb2tm2Bay
Genetic
Background		 involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgb2tm2Bay mutation (3 available); any Itgb2 mutation (50 available)
Rhohtm1Brak mutation (0 available); any Rhoh mutation (13 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system

hematopoietic system




Genotype
MGI:3826463
cx3
Allelic
Composition		 Rhohtm1Brak/Rhohtm1Brak
Tg(TcraTcrb)425Cbn/?
Genetic
Background		 involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rhohtm1Brak mutation (0 available); any Rhoh mutation (13 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:143161 )
N
• no increase in DN4 cells observed
decreased CD4-positive, alpha-beta T cell number ( J:143161 )
• severely impaired generation of CD4+ cells

hematopoietic system
decreased CD4-positive, alpha-beta T cell number ( J:143161 )
• severely impaired generation of CD4+ cells




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory