Phenotypes associated with this allele

• Allele Symbol: Vgf^tm1Vlcg
• Allele Name: targeted mutation 1, Velocigene
• Allele ID: MGI:3828156
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Vgf^tm1Vlcg/Vgf^tm1Vlcg	involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NTac	MGI:5688361
hm2	Vgf^tm1Vlcg/Vgf^tm1Vlcg	involves: 129S6/SvEvTac * C57BL/6NTac	MGI:3828162
ht3	Vgf^tm1Vlcg/Vgf^+	involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NTac	MGI:5688362

Genotype
MGI:5688361

hm1

• Allelic Composition: Vgf^tm1Vlcg/Vgf^tm1Vlcg
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal adrenal medulla morphology ( J:212045 )

• at 6-8 weeks of age, Western blot analysis revealed that chromogranin B (CgB) protein levels in the adrenal medulla are reduced to 47.3% of wild-type levels

• in contrast, adrenal chromogranin A, and secretogranin II and III levels are not significantly altered

abnormal noradrenergic chromaffin cell morphology ( J:212045 )

• at 6-8 weeks of age, the mean size of norepinephrine-containing large dense core vesicles (LDCVs) is reduced relative to wild-type adrenal chromaffin cells, and the relative number of smaller LDCVs is increased

• however, the mean number of norepinephrine-containing LDCVs per square micrometer of cytoplasm in adrenal chromaffin cells is unaffected

homeostasis/metabolism

abnormal adrenaline level ( J:212045 )

• epinephrine content is significantly increased in mutant adrenal glands relative to wild-type controls

• in contrast, cardiac muscle epinephrine levels are not significantly elevated

increased circulating adrenaline level ( J:212045 )

• plasma epinephrine levels are significantly increased relative to wild-type controls

increased noradrenaline level ( J:212045 )

• norepinephrine content is significantly increased in mutant adrenal glands relative to wild-type controls

• in contrast, plasma and cardiac muscle norepinephrine levels are not significantly elevated

nervous system

abnormal noradrenergic chromaffin cell morphology ( J:212045 )

• at 6-8 weeks of age, the mean size of norepinephrine-containing large dense core vesicles (LDCVs) is reduced relative to wild-type adrenal chromaffin cells, and the relative number of smaller LDCVs is increased

• however, the mean number of norepinephrine-containing LDCVs per square micrometer of cytoplasm in adrenal chromaffin cells is unaffected

cardiovascular system

hypertension ( J:212045 )

• homozygotes are hypertensive

increased mean systemic arterial blood pressure ( J:212045 )

• homozygotes display a significant increase in MAP relative to wild-type controls

• however, the heart rate is not significantly altered

increased systemic arterial diastolic blood pressure ( J:212045 )

• homozygotes display a significant increase in diastolic blood pressure (DBP) relative to wild-type controls

increased systemic arterial systolic blood pressure ( J:212045 )

• homozygotes display a significant increase in systolic blood pressure (SBP) relative to wild-type controls

Genotype
MGI:3828162

hm2

• Allelic Composition: Vgf^tm1Vlcg/Vgf^tm1Vlcg
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NTac

behavior/neurological

behavior/neurological phenotype ( J:143370 )

N • mice exhibit normal locomotor activities

growth/size/body

increased lean body mass ( J:143370 )

• mice are lean compared to wild-type mice

decreased body size ( J:143370 )

adipose tissue

decreased total body fat amount ( J:143370 )

• mice have decreased fat stores compared to in wild-type mice

homeostasis/metabolism

abnormal metabolism ( J:143370 )

• mice are hypermetabolic compared to wild-type mice

Genotype
MGI:5688362

ht3

• Allelic Composition: Vgf^tm1Vlcg/Vgf⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NTac

endocrine/exocrine glands

abnormal adrenal medulla morphology ( J:212045 )

• at 6-8 weeks of age, Western blot analysis revealed that chromogranin B (CgB) protein levels in the adrenal medulla are reduced to 68.7% of wild-type levels

• in contrast, adrenal chromogranin A, and secretogranin II and III levels are not significantly altered