Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^tm1(DTA)Lky
• Allele Name: targeted mutation 1, Richard M Locksley
• Allele ID: MGI:3829362
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^tm1(DTA)Lky	involves: 129P2/OlaHsd	MGI:3829363
cn2	Il13^tm1(YFP/cre)Lky/Il13^+ Il4^tm1(CD2)Mmrs/Il4^+ Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^+	involves: 129P2/OlaHsd * 129S4/SvJae	MGI:5435903
cn3	Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^+ Il13^tm1(YFP/cre)Lky/Il13^tm1(YFP/cre)Lky	involves: 129P2/OlaHsd * 129S4/SvJae	MGI:5435902
cn4	Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^+ Myf5^tm3(cre)Sor/Myf5^+	involves: 129P2/OlaHsd * 129S4/SvJaeSor	MGI:7345612
cn5	Gt(ROSA)26Sor^tm1(DTA)Lky/? Il5^tm1.1(icre)Lky/Il5^+ Rag1^tm1Mom/Rag1^tm1Mom	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6	MGI:5558890
cn6	Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^+ Tg(LHX3-cre)#Sjr/0	involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6	MGI:5576239
cn7	Epx^tm1.1(cre)Jlee/Epx^+ Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^+	involves: 129P2/OlaHsd * C57BL/6	MGI:5500049
cn8	Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^tm1(DTA)Lky Tg(Cd4-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2	MGI:3829364

Genotype
MGI:3829363

hm1

• Allelic Composition: Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^tm1(DTA)Lky
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell death ( J:133382 )

• T cells infected with a retroviral cre exhibit cell death without inducing cell death in uninfected cells

Genotype
MGI:5435903

cn2

• Allelic Composition: Il13^{tm1(YFP/cre)Lky}/Il13⁺; Il4^tm1(CD2)Mmrs/Il4⁺; Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae

immune system

decreased eosinophil cell number ( J:178986 )

• in the lungs of mice infected with N. brasiliensis

increased susceptibility to parasitic infection ( J:178986 )

• mice exhibit reduced helminthes Nippostrongylus brasiliensis expulsion compared with control mice

hematopoietic system

decreased eosinophil cell number ( J:178986 )

• in the lungs of mice infected with N. brasiliensis

Genotype
MGI:5435902

cn3

• Allelic Composition: Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor⁺; Il13^{tm1(YFP/cre)Lky}/Il13^{tm1(YFP/cre)Lky}
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae

immune system

decreased eosinophil cell number ( J:178986 )

• in the lungs of mice infected with N. brasiliensis

decreased IgG1 level ( J:178986 )

• slightly less in an anti-NP response

decreased interleukin-13 secretion ( J:178986 )

• in lung CD4+ T cells of mice infected with N. brasiliensis

decreased interleukin-5 secretion ( J:178986 )

• in lung CD4+ T cells of mice infected with N. brasiliensis

increased susceptibility to parasitic infection ( J:178986 )

• mice fail to expel the helminth Nippostrongylus brasiliensis

hematopoietic system

decreased eosinophil cell number ( J:178986 )

• in the lungs of mice infected with N. brasiliensis

decreased IgG1 level ( J:178986 )

• slightly less in an anti-NP response

Genotype
MGI:7345612

cn4

• Allelic Composition: Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor⁺; Myf5^tm3(cre)Sor/Myf5⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJaeSor

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:310908 )

• die around E15.5

craniofacial

decreased palatal shelf size ( J:310908 )

• palatal shelves are smaller

abnormal soft palate morphology ( J:310908 )

• at E15.5 almost all soft palates are fusing with the degenerating epithelial beam at the tensor veli palatini muscle level

abnormal soft palate muscle morphology ( J:310908 )

• absence of levator veli palatini, tensor veli palatini, and palatopharyngeus muscles

abnormal levator veli palatini muscle morphology ( J:310908 )

• absence of levator veli palatini muscle

abnormal palatopharyngeus muscle morphology ( J:310908 )

• absence of palatopharyngeus muscle

abnormal tensor veli palatini muscle morphology ( J:310908 )

• absence of tensor veli palatini muscle

muscle

abnormal soft palate muscle morphology ( J:310908 )

• absence of levator veli palatini, tensor veli palatini, and palatopharyngeus muscles

abnormal levator veli palatini muscle morphology ( J:310908 )

• absence of levator veli palatini muscle

abnormal palatopharyngeus muscle morphology ( J:310908 )

• absence of palatopharyngeus muscle

abnormal tensor veli palatini muscle morphology ( J:310908 )

• absence of tensor veli palatini muscle

abnormal superior pharyngeal constrictor muscle morphology ( J:310908 )

• absence of superior pharyngeal constrictor muscle

digestive/alimentary system

decreased palatal shelf size ( J:310908 )

• palatal shelves are smaller

abnormal soft palate morphology ( J:310908 )

• at E15.5 almost all soft palates are fusing with the degenerating epithelial beam at the tensor veli palatini muscle level

abnormal soft palate muscle morphology ( J:310908 )

• absence of levator veli palatini, tensor veli palatini, and palatopharyngeus muscles

abnormal levator veli palatini muscle morphology ( J:310908 )

• absence of levator veli palatini muscle

abnormal palatopharyngeus muscle morphology ( J:310908 )

• absence of palatopharyngeus muscle

abnormal tensor veli palatini muscle morphology ( J:310908 )

• absence of tensor veli palatini muscle

growth/size/body

decreased palatal shelf size ( J:310908 )

• palatal shelves are smaller

abnormal soft palate morphology ( J:310908 )

• at E15.5 almost all soft palates are fusing with the degenerating epithelial beam at the tensor veli palatini muscle level

abnormal soft palate muscle morphology ( J:310908 )

• absence of levator veli palatini, tensor veli palatini, and palatopharyngeus muscles

abnormal levator veli palatini muscle morphology ( J:310908 )

• absence of levator veli palatini muscle

abnormal palatopharyngeus muscle morphology ( J:310908 )

• absence of palatopharyngeus muscle

abnormal tensor veli palatini muscle morphology ( J:310908 )

• absence of tensor veli palatini muscle

respiratory system

abnormal superior pharyngeal constrictor muscle morphology ( J:310908 )

• absence of superior pharyngeal constrictor muscle

Genotype
MGI:5558890

cn5

• Allelic Composition: Gt(ROSA)26Sor^tm1(DTA)Lky/?; Il5^{tm1.1(icre)Lky}/Il5⁺; Rag1^tm1Mom/Rag1^tm1Mom
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6

immune system

abnormal immune system physiology ( J:206097 )

• no increase in type 2 innate lymphoid cells (ILC2) or induction of eotaxin-1 (Ccl11) is detected in lungs in response to administration of Il2 and Il33 in contrast to control mice lacking DTA expression which have a higher ILC2 population and Ccl11 expression

Genotype
MGI:5576239

cn6

• Allelic Composition: Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor⁺; Tg(LHX3-cre)#Sjr/0
• Genetic Background: involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6

mortality/aging

lethality throughout fetal growth and development, incomplete penetrance ( J:211401 )

• viable double mutant embryos are recovered in significantly reduced numbers at E14.5 and E17.5 compared to cre-negative/R26^DTA embryos

growth/size/body

decreased body size ( J:211401 )

• the few animals surviving past birth are smaller than littermates

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:211401 )

N • ACTH hormone levels are not significantly altered in mutants at E14.5

N • at E17.5 and P0, numbers of ACTH, TPIT, PIT-1 (ie. thyrotropes, somatotropes, and lactotropes), PRL, and GH-positive cells in the anterior pituitary are not significantly reduced compared to controls

abnormal adenohypophysis morphology ( J:211401 )

• at E14.5, cells positive for alphaGSU expression (ie. gonadotrophs) are reduced in the caudomedial portion of the anterior pituitary

• GATA2-positive cells are absent from the caudomedial region of the pituitary but are still present in the rostral tip

decreased gonadotroph cell number ( J:211401 )

• at E14.5, cells positive for alphaGSU expression (ie. gonadotropes) are reduced in the caudomedial portion of the anterior pituitary

• in animals at P0, LH beta-positive gonadotrope cells are decreased by about 90% compared to controls; FSH beta-positive gonadotrope cells are decreased slightly also

• at P0, numbers of LH/SF1 or FSH/SF1 double positive cells are significantly decreased

• at P0, about 90% of bihormonal (LH beta/FSH beta positive) cells are missing compared to controls

nervous system

abnormal adenohypophysis morphology ( J:211401 )

• at E14.5, cells positive for alphaGSU expression (ie. gonadotrophs) are reduced in the caudomedial portion of the anterior pituitary

• GATA2-positive cells are absent from the caudomedial region of the pituitary but are still present in the rostral tip

decreased gonadotroph cell number ( J:211401 )

• at E14.5, cells positive for alphaGSU expression (ie. gonadotropes) are reduced in the caudomedial portion of the anterior pituitary

• in animals at P0, LH beta-positive gonadotrope cells are decreased by about 90% compared to controls; FSH beta-positive gonadotrope cells are decreased slightly also

• at P0, numbers of LH/SF1 or FSH/SF1 double positive cells are significantly decreased

• at P0, about 90% of bihormonal (LH beta/FSH beta positive) cells are missing compared to controls

abnormal spinal cord morphology ( J:211401 )

• numbers of Lhx-positive interneurons are decreased in the V2 interneuron region of the spinal cord at E14.5, but populations of motorneurons (ventral) are not affected

• at E14.5, V2a interneurons expressing LHX3 and CHX10 are reduced in number

reproductive system

male infertility ( J:211401 )

♂ • of 2 surviving male mutants, one was infertile

Genotype
MGI:5500049

cn7

• Allelic Composition: Epx^{tm1.1(cre)Jlee}/Epx⁺; Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

hematopoietic system

decreased eosinophil cell number ( J:198489 )

• peripheral blood is deficient in eosinophils relative to wild-type (0.19% vs 1.85%)

• other non-eosinophilic circulating cell populations are unchanged in cell number and relative composition in mutants

immune system

decreased eosinophil cell number ( J:198489 )

• peripheral blood is deficient in eosinophils relative to wild-type (0.19% vs 1.85%)

• other non-eosinophilic circulating cell populations are unchanged in cell number and relative composition in mutants

Genotype
MGI:3829364

cn8

• Allelic Composition: Gt(ROSA)26Sor^tm1(DTA)Lky/Gt(ROSA)26Sor^tm1(DTA)Lky; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA/2

immune system

immune system phenotype ( J:133382 )

N • despite the reduction in CD4+ and CD8+ T cells, the pool of memory T cells is normal

abnormal CD8-positive, alpha-beta T cell differentiation ( J:133382 )

• CD8+ cells exhibit an immature phenotype due to incomplete thymic maturation

abnormal T cell receptor V(D)J recombination ( J:133382 )

• T cells exhibit incomplete repertoires of TCR specificities with a biased Vbeta chain usage unlike in wild-type cells

decreased lymphocyte cell number ( J:133382 )

• the naive T cell population in the peripheral pool is depleted and mice remain lymphopenic over time

decreased double-positive T cell number ( J:133382 )

• by 50%

decreased CD4-positive, alpha-beta T cell number ( J:133382 )

• CD4+ cells in the thymus are almost completely absent

• fewer CD4+ and CD8+ cells are found in the lymph, nodes, spleen and blood compared to in wild-type mice with remaining cells accounted for by incomplete recombination

decreased CD8-positive, alpha-beta T cell number ( J:133382 )

• fewer immature CD8+ T cells are found in the thymus compared to in wild-type mice

• fewer CD4+ and CD8+ cells are found in the lymph, nodes, spleen and blood compared to in wild-type mice with remaining cells accounted for by incomplete recombination

decreased NK T cell number ( J:133382 )

• mice exhibit a loss of CD4+ NK T cells in the liver compared to in wild-type mice

decreased regulatory T cell number ( J:133382 )

abnormal spleen morphology ( J:133382 )

• the spleen has a smaller T cell zone than in wild-type mice

• however, overall architecture is normal

abnormal CD4-positive, alpha-beta T cell physiology ( J:133382 )

• following infection with N. brasiliensis, CD4+ T cells preferentially differentiate towards Th1 and Th17 cells unlike similarly treated wild-type mice whose CD4+ T cells preferentially differentiate towards Th2 cells

abnormal lymph node T cell domain morphology ( J:133382 )

• the lymph nodes have smaller T cell zones than in wild-type mice

• however, overall architecture is normal

increased susceptibility to parasitic infection ( J:133382 )

• mice infected with N. brasiliensis exhibit impaired effector cell recruitment and worm expulsion with 4-fold higher worm counts in the small intestine compared to in similarly treated wild-type mice

• nine days after infection with N. brasiliensis, mice exhibit reduced basophil and eosinophil frequencies in the blood and lungs compared to similarly treated wild-type mice

• after infection with N. brasiliensis, total numbers of eosinophils, basophils and CD4+ T cells in the lungs are 5- to 10-fold lower than in similarly treated wild-type mice

• following infection with N. brasiliensis, CD4+ T cells preferentially differentiate towards Th1 and Th17 cells unlike similarly treated wild-type mice whose CD4+ T cells preferentially differentiate towards Th2 cells

• however, IgE levels in mice infected with N. brasiliensis are normal

hematopoietic system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:133382 )

• CD8+ cells exhibit an immature phenotype due to incomplete thymic maturation

abnormal T cell receptor V(D)J recombination ( J:133382 )

• T cells exhibit incomplete repertoires of TCR specificities with a biased Vbeta chain usage unlike in wild-type cells

decreased lymphocyte cell number ( J:133382 )

• the naive T cell population in the peripheral pool is depleted and mice remain lymphopenic over time

decreased double-positive T cell number ( J:133382 )

• by 50%

decreased CD4-positive, alpha-beta T cell number ( J:133382 )

• CD4+ cells in the thymus are almost completely absent

• fewer CD4+ and CD8+ cells are found in the lymph, nodes, spleen and blood compared to in wild-type mice with remaining cells accounted for by incomplete recombination

decreased CD8-positive, alpha-beta T cell number ( J:133382 )

• fewer immature CD8+ T cells are found in the thymus compared to in wild-type mice

• fewer CD4+ and CD8+ cells are found in the lymph, nodes, spleen and blood compared to in wild-type mice with remaining cells accounted for by incomplete recombination

decreased NK T cell number ( J:133382 )

• mice exhibit a loss of CD4+ NK T cells in the liver compared to in wild-type mice

decreased regulatory T cell number ( J:133382 )

abnormal spleen morphology ( J:133382 )

• the spleen has a smaller T cell zone than in wild-type mice

• however, overall architecture is normal

abnormal CD4-positive, alpha-beta T cell physiology ( J:133382 )

• following infection with N. brasiliensis, CD4+ T cells preferentially differentiate towards Th1 and Th17 cells unlike similarly treated wild-type mice whose CD4+ T cells preferentially differentiate towards Th2 cells