Phenotypes associated with this allele

• Allele Symbol: Glrx3^{Gt(RRF094)Byg}
• Allele Name: gene trap RRF094, BayGenomics
• Allele ID: MGI:3829374
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Glrx3^Gt(RRF094)Byg/Glrx3^Gt(RRF094)Byg	involves: 129P2/OlaHsd * C57BL/6	MGI:3829375
ht2	Glrx3^Gt(RRF094)Byg/Glrx3^+	involves: 129P2/OlaHsd * C57BL/6	MGI:3829376

Genotype
MGI:3829375

hm1

• Allelic Composition: Glrx3^{Gt(RRF094)Byg}/Glrx3^{Gt(RRF094)Byg}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:143577 )

• mice die between E12.5 and E14.5; none are recovered past E14.5

embryonic lethality during organogenesis, complete penetrance ( J:190794 )

• by E13.5

embryonic lethality during organogenesis, incomplete penetrance ( J:143577 )

• mice die between E12.5 and E14.5

cardiovascular system

pericardial effusion ( J:190794 )

• in some mice at E12.5

intracranial hemorrhage ( J:143577 )

• at E12.5, mice exhibit hemorrhages in the head

growth/size/body

decreased embryo size ( J:143577 , J:190794 )

• at E12.5 (J:143577)

• at E12.5 (J:190794)

embryo

decreased embryo size ( J:143577 , J:190794 )

• at E12.5 (J:143577)

• at E12.5 (J:190794)

open neural tube ( J:190794 )

• open anterior neural tube in some mice at E12.5

cellular

binucleate ( J:190794 )

• more binucleated cells are seen in cultured MEFs

abnormal cell cycle ( J:190794 )

• in culture MEFs show an increase in the proportion of cells in the G2/M phase and more binucleated cells are seen

abnormal cell death ( J:190794 )

• MEFs do not survive beyond passage 4

decreased fibroblast proliferation ( J:190794 )

• in MEFs

nervous system

intracranial hemorrhage ( J:143577 )

• at E12.5, mice exhibit hemorrhages in the head

open neural tube ( J:190794 )

• open anterior neural tube in some mice at E12.5

homeostasis/metabolism

pericardial effusion ( J:190794 )

• in some mice at E12.5

Genotype
MGI:3829376

ht2

• Allelic Composition: Glrx3^{Gt(RRF094)Byg}/Glrx3⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

cardiovascular system

decreased cardiac muscle contractility ( J:143577 )

• cardiomyocytes exhibit a 23% decrease in cell shortening, a 29% decrease in maximal rate of contraction, and a 36% decrease in maximal rate of relaxation compared to wild-type cells

• following transverse aortic banding, decreased cardiomyocyte contractility is partially restored but not to wild-type levels

abnormal cardiac muscle relaxation ( J:143577 )

• cardiomyocytes exhibit a 36% decrease in maximal rate of relaxation compared to wild-type cells

abnormal myocardial fiber physiology ( J:143577 )

• transient calcium ion decay in cardiomyocytes is increased 29% compared to in wild-type cells

• cardiomyocyte sarcoplasmic reticulum re-uptake of calcium ions is reduced 39% compared to in wild-type cells

• however, baseline calcium ion content is normal

increased response of heart to induced stress ( J:143577 )

• following transverse aortic banding, mice exhibit a 65% increase in heart weight compared to a 38% increase in similarly treated wild-type mice with increased ventricular cross-sectional area

• following transverse aortic banding, decreased cardiomyocyte contractility is partially restored but not to wild-type levels

muscle

decreased cardiac muscle contractility ( J:143577 )

• cardiomyocytes exhibit a 23% decrease in cell shortening, a 29% decrease in maximal rate of contraction, and a 36% decrease in maximal rate of relaxation compared to wild-type cells

• following transverse aortic banding, decreased cardiomyocyte contractility is partially restored but not to wild-type levels

abnormal cardiac muscle relaxation ( J:143577 )

• cardiomyocytes exhibit a 36% decrease in maximal rate of relaxation compared to wild-type cells

homeostasis/metabolism

increased response of heart to induced stress ( J:143577 )

• following transverse aortic banding, mice exhibit a 65% increase in heart weight compared to a 38% increase in similarly treated wild-type mice with increased ventricular cross-sectional area

• following transverse aortic banding, decreased cardiomyocyte contractility is partially restored but not to wild-type levels