immune system
• peritoneal macrophages from the index mouse, on three separate occasions, exhibited reduced production of tumor necrosis factor (TNF) alpha in response to the TLR2/6 ligand MALP-2 (macrophage activating lipopeptide 2)
• mutant macrophages exhibited a normal TNF response to signaling via other toll-like receptor (TLR) ligands: the TLR2/1 ligand Pam3CSK4 (triacyl lipopeptide); the TLR4 ligand lipopolysaccharide (LPS); the TLR9 ligand CpG-DNA (single-stranded DNA); the TLR7 ligand resiquimod/R-848 (single-stranded RNA mimetic); and the TLR3 ligand poly I:C (double-stranded RNA)
• although mutant macrophages showed a normal TNF alpha response to the TLR2/6 ligand peptidoglycan (PGN), this is likely due to contamination by a TLR1/2 ligand, as TLR6 deficient mice respond normally to this ligand, whereas TLR2 mutant mice do not
|
• peritoneal macrophages from the index mouse, on three separate occasions, exhibited reduced production of tumor necrosis factor (TNF) alpha in response to the TLR2/6 ligand MALP-2 (macrophage activating lipopeptide 2)
• mutant macrophages exhibited a normal TNF response to signaling via other toll-like receptor (TLR) ligands: the TLR2/1 ligand Pam3CSK4 (triacyl lipopeptide); the TLR4 ligand lipopolysaccharide (LPS); the TLR9 ligand CpG-DNA (single-stranded DNA); the TLR7 ligand resiquimod/R-848 (single-stranded RNA mimetic); and the TLR3 ligand poly I:C (double-stranded RNA)
• although mutant macrophages showed a normal TNF alpha response to the TLR2/6 ligand peptidoglycan (PGN), this is likely due to contamination by a TLR1/2 ligand, as TLR6 deficient mice respond normally to this ligand, whereas TLR2 mutant mice do not
|
reproductive system
|
• the index male mouse failed to breed by 6 months of age; this may have been due to an independent mutation
|
hematopoietic system
• peritoneal macrophages from the index mouse, on three separate occasions, exhibited reduced production of tumor necrosis factor (TNF) alpha in response to the TLR2/6 ligand MALP-2 (macrophage activating lipopeptide 2)
• mutant macrophages exhibited a normal TNF response to signaling via other toll-like receptor (TLR) ligands: the TLR2/1 ligand Pam3CSK4 (triacyl lipopeptide); the TLR4 ligand lipopolysaccharide (LPS); the TLR9 ligand CpG-DNA (single-stranded DNA); the TLR7 ligand resiquimod/R-848 (single-stranded RNA mimetic); and the TLR3 ligand poly I:C (double-stranded RNA)
• although mutant macrophages showed a normal TNF alpha response to the TLR2/6 ligand peptidoglycan (PGN), this is likely due to contamination by a TLR1/2 ligand, as TLR6 deficient mice respond normally to this ligand, whereas TLR2 mutant mice do not
|