Phenotypes associated with this allele

• Allele Symbol: Nfatc1^tm3Glm
• Allele Name: targeted mutation 3, Laurie H Glimcher
• Allele ID: MGI:3831720
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nfatc1^tm3Glm/Nfatc1^tm3Glm Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129P2/OlaHsd * C57BL/6	MGI:3831755
cn2	Nfatc1^tm3Glm/Nfatc1^tm3Glm Sh3bp2^tm1Bjro/Sh3bp2^tm1Bjro Tg(Mx1-cre)1Cgn/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:3831754
cn3	Nfatc1^tm3Glm/Nfatc1^tm3Glm Tg(Mx1-cre)1Cgn/0	involves: C57BL/6 * CBA	MGI:3831753
cn4	Hivep3^tm1Glm/Hivep3^tm1Glm Nfatc1^tm3Glm/Nfatc1^tm3Glm Tg(Mx1-cre)1Cgn/0	involves: C57BL/6 * CBA	MGI:5550521
cn5	Nfatc1^tm3Glm/Nfatc1^tm3Glm Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/?	involves: C57BL/6 * CD-1	MGI:3831756

Genotype
MGI:3831755

cn1

• Allelic Composition: Nfatc1^tm3Glm/Nfatc1^tm3Glm; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

skeleton phenotype ( J:144598 )

N • no alterations in bone density or osteoclast differentiation are observed due to a lack of cre expression in osteoclasts

Genotype
MGI:3831754

cn2

• Allelic Composition: Nfatc1^tm3Glm/Nfatc1^tm3Glm; Sh3bp2^tm1Bjro/Sh3bp2^tm1Bjro; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

skeleton

skeleton phenotype ( J:144598 )

N • induction of cre at 10 days of age prevents the diffuse bone loss and focal cystic changes characteristic of mice homozygote for the Sh3bp2^tm1Bjro allele

abnormal osteoclast differentiation ( J:144598 )

• bone marrow cells fail to differentiate into osteoclasts in vitro

decreased osteoclast cell number ( J:144598 )

• a dramatic reduction in the number of TRAP⁺ cells is found iin the proximal humerus and distal femur

immune system

abnormal osteoclast differentiation ( J:144598 )

• bone marrow cells fail to differentiate into osteoclasts in vitro

stomach inflammation ( J:144598 )

• widespread inflammation is observed

decreased osteoclast cell number ( J:144598 )

• a dramatic reduction in the number of TRAP⁺ cells is found iin the proximal humerus and distal femur

lymph node inflammation ( J:144598 )

• widespread inflammation is observed

liver inflammation ( J:144598 )

• widespread inflammation is observed

lung inflammation ( J:144598 )

• widespread inflammation is observed

digestive/alimentary system

stomach inflammation ( J:144598 )

• widespread inflammation is observed

liver/biliary system

liver inflammation ( J:144598 )

• widespread inflammation is observed

respiratory system

lung inflammation ( J:144598 )

• widespread inflammation is observed

hematopoietic system

abnormal osteoclast differentiation ( J:144598 )

• bone marrow cells fail to differentiate into osteoclasts in vitro

decreased osteoclast cell number ( J:144598 )

• a dramatic reduction in the number of TRAP⁺ cells is found iin the proximal humerus and distal femur

cellular

abnormal osteoclast differentiation ( J:144598 )

• bone marrow cells fail to differentiate into osteoclasts in vitro

Genotype
MGI:3831753

cn3

• Allelic Composition: Nfatc1^tm3Glm/Nfatc1^tm3Glm; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * CBA

skeleton

abnormal osteoclast differentiation ( J:144598 )

• after cre induction, osteoclasts fail to develop from bone marrow cells in response to culturing with either osteoblasts, RANKL or M-CSF

• normal numbers of osteoclast progenitors are present in the bone marrow suggesting a defect in differentiation

short lower incisors ( J:144598 )

• some mice have overgrowth of the upper incisors with short or absent lower incisors

long upper incisors ( J:144598 )

• some mice have overgrowth of the upper incisors with short or absent lower incisors

absent lower incisors ( J:144598 )

• some mice have overgrowth of the upper incisors with short or absent lower incisors

abnormal mandibular condyloid process morphology ( J:144598 )

• lower mandible has a marked retention of hypertrophic cartilage when cre is induced at 10 days of age

decreased osteoclast cell number ( J:144598 )

• the number of osteoclasts per mm bone surface is severely reduced in mice with cre induction at 10 days of age

increased width of hypertrophic chondrocyte zone ( J:144598 )

• cre induction at 10 days of age leads to growth plate dysplasia, characterized by an increase in thickness, acellular areas, and the retention of hypertrophic chondrocytes beyond the chondro-osseous junction

decreased length of long bones ( J:144598 )

• cre-induction at 10 days of age leads to shortened long bones by 5 months of age

short femur ( J:144598 )

• cre induction at 10 days of age leads to short, club-shaped femurs, with higher radiodensity at the distal portion

short tibia ( J:144598 )

• cre induction at 10 days of age leads to short, club-shaped tibias, with higher radiodensity at the proximal portion

osteopetrosis ( J:144598 )

• the bone volume to total volume fraction of femurs is twice that of controls when cre is induced at 10 days of age

• cre induction at 10 days of age also leads to other features of severe osteopetrosis, including improperly shaped long bones, and a failure to resorb calcified cartilage resulting in aberrant endochondral growth and ossification

abnormal endochondral bone ossification ( J:144598 )

• cre induction at 10 days of age leads to a massive increase in calcified cartilage adjacent to the growth plates

craniofacial

short lower incisors ( J:144598 )

• some mice have overgrowth of the upper incisors with short or absent lower incisors

long upper incisors ( J:144598 )

• some mice have overgrowth of the upper incisors with short or absent lower incisors

absent lower incisors ( J:144598 )

• some mice have overgrowth of the upper incisors with short or absent lower incisors

abnormal mandibular condyloid process morphology ( J:144598 )

• lower mandible has a marked retention of hypertrophic cartilage when cre is induced at 10 days of age

limbs/digits/tail

short femur ( J:144598 )

• cre induction at 10 days of age leads to short, club-shaped femurs, with higher radiodensity at the distal portion

short tibia ( J:144598 )

• cre induction at 10 days of age leads to short, club-shaped tibias, with higher radiodensity at the proximal portion

hematopoietic system

abnormal osteoclast differentiation ( J:144598 )

• after cre induction, osteoclasts fail to develop from bone marrow cells in response to culturing with either osteoblasts, RANKL or M-CSF

• normal numbers of osteoclast progenitors are present in the bone marrow suggesting a defect in differentiation

decreased osteoclast cell number ( J:144598 )

• the number of osteoclasts per mm bone surface is severely reduced in mice with cre induction at 10 days of age

immune system

abnormal osteoclast differentiation ( J:144598 )

• after cre induction, osteoclasts fail to develop from bone marrow cells in response to culturing with either osteoblasts, RANKL or M-CSF

• normal numbers of osteoclast progenitors are present in the bone marrow suggesting a defect in differentiation

decreased osteoclast cell number ( J:144598 )

• the number of osteoclasts per mm bone surface is severely reduced in mice with cre induction at 10 days of age

cellular

abnormal osteoclast differentiation ( J:144598 )

• after cre induction, osteoclasts fail to develop from bone marrow cells in response to culturing with either osteoblasts, RANKL or M-CSF

• normal numbers of osteoclast progenitors are present in the bone marrow suggesting a defect in differentiation

growth/size/body

short lower incisors ( J:144598 )

• some mice have overgrowth of the upper incisors with short or absent lower incisors

long upper incisors ( J:144598 )

• some mice have overgrowth of the upper incisors with short or absent lower incisors

absent lower incisors ( J:144598 )

• some mice have overgrowth of the upper incisors with short or absent lower incisors

Genotype
MGI:5550521

cn4

• Allelic Composition: Hivep3^tm1Glm/Hivep3^tm1Glm; Nfatc1^tm3Glm/Nfatc1^tm3Glm; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * CBA

skeleton

increased bone volume ( J:184764 )

Genotype
MGI:3831756

cn5

• Allelic Composition: Nfatc1^tm3Glm/Nfatc1^tm3Glm; Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/?
• Genetic Background: involves: C57BL/6 * CD-1

skeleton

skeleton phenotype ( J:144598 )

N • mice do not display an osteopetrotic phenotype