Phenotypes associated with this allele

• Allele Symbol: Itga9^tm2Des
• Allele Name: targeted mutation 2, Dean Sheppard
• Allele ID: MGI:3832228
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Itga9^tm2Des/Itga9^tm2Des	involves: 129S4/SvJae * C57BL/6	MGI:3839290
cn2	Itga9^tm2Des/Itga9^tm2Des	involves: 129S4/SvJae * C57BL/6	MGI:5448445
cn3	Itga9^tm2Des/Itga9^tm2Des Tg(ACTA2-rtTA)#Des/0 Tg(tetO-cre)1Jaw/0	involves: 129 * 129S4/SvJae * C57BL/6	MGI:5448443
cn4	Itga9^tm2Des/Itga9^tm2Des Tg(KRT14-cre)1Amc/?	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:3832489

Genotype
MGI:3839290

hm1

• Allelic Composition: Itga9^tm2Des/Itga9^tm2Des
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:144854 )

• mice are viable and appear normal

Genotype
MGI:5448445

cn2

• Allelic Composition: Itga9^tm2Des/Itga9^tm2Des
• Genetic Background: involves: 129S4/SvJae * C57BL/6

respiratory system

abnormal respiratory system morphology ( J:190074 )

• methacholine-treated mice infected with a cre-expressing adenovirus exhibit increased narrowing in airways compared with control mice

abnormal respiratory system physiology ( J:190074 )

• lung slices infected with a cre-expressing adenovirus then treated with methacholine exhibit increased in vitro force generation compared with control rings

Genotype
MGI:5448443

cn3

• Allelic Composition: Itga9^tm2Des/Itga9^tm2Des; Tg(ACTA2-rtTA)#Des/0; Tg(tetO-cre)1Jaw/0
• Genetic Background: involves: 129 * 129S4/SvJae * C57BL/6

respiratory system

respiratory system phenotype ( J:190074 )

N • mice exhibit normal airway epithelium morphology and no evidence of inflammation

abnormal respiratory system morphology ( J:190074 )

• methacholine-treated mice exhibit increased narrowing in airways compared with control mice

• lung slices treated with methacholine in vitro exhibit increased narrowing compared with control slices

• spermine has no increase in contraction unlike in control samples

• however, putrescine has no effect on airway contraction

abnormal respiratory system physiology ( J:190074 )

• excised tracheal rings (with or without epithelium) treated with methacholine exhibit increased in vitro force generation compared with control rings

• lung slices and tracheal rings exhibit increased contractile response to 5-HT compared with control samples

increased airway responsiveness ( J:190074 )

• in response to acetylcholine

Genotype
MGI:3832489

cn4

• Allelic Composition: Itga9^tm2Des/Itga9^tm2Des; Tg(KRT14-cre)1Amc/?
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

vision/eye

vision/eye phenotype ( J:144854 )

N • eye size normal

N • corneal clarity normal

abnormal cornea epithelium morphology ( J:144854 )

• basal cells are shorter than in controls and are wider

decreased cornea epithelium thickness ( J:144854 )

• thinner than controls but with a similar number of layers

decreased cornea thickness ( J:144854 )

• cornea is thinner, 50um as compared to 62um

homeostasis/metabolism

abnormal wound healing ( J:144854 )

• reduced proliferation of keratinocytes at 2 and 4 days after wounding

• migration of epithelial cells into the wound area is normal