Phenotypes associated with this allele

• Allele Symbol: Ccdc88a^tm1Mat
• Allele Name: targeted mutation 1, Masahide Takahashi
• Allele ID: MGI:3835852
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ccdc88a^tm1Mat/Ccdc88a^tm1Mat	Not Specified	MGI:3835860
ht2	Ccdc88a^tm1Mat/Ccdc88a^+	Not Specified	MGI:5616118

Genotype
MGI:3835860

hm1

• Allelic Composition: Ccdc88a^tm1Mat/Ccdc88a^tm1Mat
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:145672 )

• by P25

nervous system

nervous system phenotype ( J:173379 )

N • at P0, brain architecture is normal

abnormal neuron differentiation ( J:173379 )

• neuroblast apoptosis in the subventricular zone is increased compared to in wild-type mice

• however, neuroblast proliferation is normal

abnormal neuronal precursor cell migration ( J:173379 )

• neuroblasts fail to migrate properly to the olfactory bulb and accumulate in the subventricular zone and halfway through the rostral migratory stream unlike in wild-type mice

• neuroblasts fail to segregate from astrocytes unlike in wild-type mice

• neuroblasts transplanted into the subventricular zone of wild-type mice exhibit impaired migration unlike wild-type

• neuroblasts fail to undergo chain migration in culture unlike wild-type cells

decreased brain size ( J:173379 )

• mild at P15

abnormal neocortex morphology ( J:173379 )

abnormal olfactory bulb morphology ( J:173379 )

abnormal olfactory bulb development ( J:173379 )

abnormal rostral migratory stream morphology ( J:173379 )

• neuroblasts fail to migrate properly to the olfactory bulb and accumulate in the subventricular zone and halfway through the rostral migratory stream unlike in wild-type mice

abnormal olfactory bulb glomerular layer morphology ( J:173379 )

• disorganized

abnormal olfactory bulb granule cell layer morphology ( J:173379 )

• disorganized

abnormal olfactory bulb mitral cell layer morphology ( J:173379 )

• disorganized

abnormal olfactory bulb subventricular zone morphology ( J:173379 )

• neuroblasts fail to migrate properly to the olfactory bulb and accumulate in the subventricular zone and halfway through the rostral migratory stream unlike in wild-type mice

small olfactory bulb ( J:173379 )

• at P0 and P15

abnormal GABAergic neuron morphology ( J:173379 )

• at P15, fewer cortical GABAergic interneurons are present compared to in wild-type mice

• at P15, fewer calbindin+ interneurons are present in cortical layers IV-VI compared to in wild-type mice

decreased neuron number ( J:173379 )

• at P15, fewer cortical GABAergic interneurons are present compared to in wild-type mice

cardiovascular system

abnormal retina vasculature morphology ( J:145672 )

• between P3 and P7, retinal vasculature exhibits a 60% decrease in vascular sprouting compared to in wild-type mice with incomplete coverage at the retinal periphery

• at P18, intermediate and deep retinal vascular layers are poorly developed unlike in wild-type mice

• however, tip cells and pericytes in the developing retina are normal

decreased angiogenesis ( J:145672 )

• the length of blood vessels in the brain cortex is decreased compared to in wild-type mice

• capillary outgrowth of P7 aortic rings treated with VEGF-A is impaired compared to in similarly treated wild-type aortic rings

growth/size/body

decreased body weight ( J:145672 )

• beginning at P7

weight loss ( J:145672 )

• after P7 to P8

vision/eye

abnormal retina vasculature morphology ( J:145672 )

• between P3 and P7, retinal vasculature exhibits a 60% decrease in vascular sprouting compared to in wild-type mice with incomplete coverage at the retinal periphery

• at P18, intermediate and deep retinal vascular layers are poorly developed unlike in wild-type mice

• however, tip cells and pericytes in the developing retina are normal

cellular

abnormal neuron differentiation ( J:173379 )

• neuroblast apoptosis in the subventricular zone is increased compared to in wild-type mice

• however, neuroblast proliferation is normal

abnormal neuronal precursor cell migration ( J:173379 )

• neuroblasts fail to migrate properly to the olfactory bulb and accumulate in the subventricular zone and halfway through the rostral migratory stream unlike in wild-type mice

• neuroblasts fail to segregate from astrocytes unlike in wild-type mice

• neuroblasts transplanted into the subventricular zone of wild-type mice exhibit impaired migration unlike wild-type

• neuroblasts fail to undergo chain migration in culture unlike wild-type cells

Genotype
MGI:5616118

ht2

• Allelic Composition: Ccdc88a^tm1Mat/Ccdc88a⁺
• Genetic Background: Not Specified

behavior/neurological

impaired contextual conditioning behavior ( J:217412 )

• in the fear conditioning test, 7-20 week old mice show context-dependent fear memory impairment 24 hours, but not 1 hour, after fear conditioning, although no difference is seen in freezing time in the conditioning phase

• however, mice show normal tone-dependent freezing 24 hours after the conditioning

abnormal long-term object recognition memory ( J:217412 )

• in the novel object recognition test, mice show a decrease in the exploratory preference for novel objects in the retention session performed 24 hours after the training session, indicating impaired nonspatial memory

impaired spatial learning ( J:217412 )

• in the Morris water maze, mice take a longer path length to find the hidden platform during training trails, however no difference is seen in the visible test on day 7 and in the swimming velocity during the training trials

• mice show impairments of time spend and the number of platform crossings in the target quadrant in the probe test of the Morris water maze

• mice, however show normal short-term memory in the Y-maze test and normal behavior in the open field, elevated-plus maze, forced-swimming, locomotor pattern in a novel environment, sensorimotor gating and sucrose-preference tests

nervous system

abnormal excitatory postsynaptic currents ( J:217412 )

• the ratio of excitatory postsynaptic currents (EPSCs) mediated by NMDA receptor to AMPA receptor in dentate granule cells of hippocampal slices is lower

• however, mice show a normal decay time constant of NMDA receptor-mediated EPSCs

reduced long-term potentiation ( J:217412 )

• magnitude of LTP is smaller at 55-60 min after the TET in the hippocampus