Phenotypes associated with this allele

• Allele Symbol: Tg(Cdh5-cre)1Spe
• Allele Name: transgene insertion 1, Nancy A Speck
• Allele ID: MGI:3836418
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Plvap^tm1.1Rvst/Plvap^tm1.1Rvst Tg(Cdh5-cre)1Spe/0	B6.Cg-Plvap^tm1.1Rvst Tg(Cdh5-cre)1Spe	MGI:5473520
cn2	Runx1^tm3Spe/Runx1^tm3Spe Tg(Cdh5-cre)1Spe/0	involves: 129S1/SvImJ * 129S4/SvJae * C57BL/6	MGI:3836436
cn3	Plvap^tm1.1Rvst/Plvap^tm1.1Rvst Tg(Cdh5-cre)1Spe/0	involves: 129S1/SvImJ * 129S4/SvJae * C57BL/6 * C57BL/6J	MGI:5473519
cn4	Best3^tm1.1Zhoj/Best3^tm1.1Zhoj Tg(Cdh5-cre)1Spe/0	involves: 129S1/SvImJ * C57BL/6J	MGI:7537042

Genotype
MGI:5473520

cn1

• Allelic Composition: Plvap^tm1.1Rvst/Plvap^tm1.1Rvst; Tg(Cdh5-cre)1Spe/0
• Genetic Background: B6.Cg-Plvap^tm1.1Rvst Tg(Cdh5-cre)1Spe

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

preweaning lethality, complete penetrance ( J:191046 )

• Background Sensitivity: display 100% lethality by P2 on a congenic C57BL/6 background unlike mice on a mixed background where about 20-30% of mice survive to 6-7 months of age

Genotype
MGI:3836436

cn2

• Allelic Composition: Runx1^tm3Spe/Runx1^tm3Spe; Tg(Cdh5-cre)1Spe/0
• Genetic Background: involves: 129S1/SvImJ * 129S4/SvJae * C57BL/6

mortality/aging

lethality throughout fetal growth and development, incomplete penetrance ( J:145700 )

• 65% of embryos die between E12.5 and E15.5

hematopoietic system

anemia ( J:145700 )

• 10% of E12.5 embryos have a pale liver due to anemia

abnormal hematopoietic stem cell morphology ( J:145700 )

• hematopoietic stem cell first derive as clusters of Kit^high cells from the endothelium of dorsal aorta and vitelline and umbilical arteries of E10.5 conceptus

• these clusters are absent in mutant E10.5 conceptus and there is a 90-fold decrease in the Kit^high cells

decreased hematopoietic stem cell number ( J:145700 )

• there is about a 8-fold decrease in CFUs from the yolk sac, vitelline and umbilical arteries, placenta and fetal liver

• over 99% of the CFUs that do develop in culture have one functional Runx1 allele

• decreased hematopoietic stem cell numbers are reflected in poor engraftment of mutant bone marrow cells into irradiated recipients

• CFUs cultured from bone marrow of transplant recipients are much reduced compared to controls and over 99% also have one functional Runx1 allele

cardiovascular system

internal hemorrhage ( J:145700 )

• 10% of E12.5 embryos have hemorrhaging within the central nervous system

liver/biliary system

pale liver ( J:145700 )

• 10% of E12.5 embryos have a pale liver due to anemia

Genotype
MGI:5473519

cn3

• Allelic Composition: Plvap^tm1.1Rvst/Plvap^tm1.1Rvst; Tg(Cdh5-cre)1Spe/0
• Genetic Background: involves: 129S1/SvImJ * 129S4/SvJae * C57BL/6 * C57BL/6J

mortality/aging

lethality, incomplete penetrance ( J:191046 )

• Background Sensitivity: display 100% lethality by P2 on a congenic C57BL/6 background unlike mice on a mixed background where about 20-30% of mice survive to 6-7 months of age

cardiovascular system

abnormal capillary morphology ( J:191046 )

• lack diaphragms in endothelial fenestrae, transendothelial channels, and caveolae in pancreas, intestine, kidney, lung and adrenals

increased vascular permeability ( J:191046 )

• leakage of Evans Blue dye is dramatically increased in organs with fenestrated endothelial cells

• leakage of Evans Blue dye is highest in the intestine

homeostasis/metabolism

abnormal circulating lipid level ( J:191046 )

decreased circulating HDL cholesterol level ( J:191046 )

• significantly decreased at 4 weeks of age and does not vary much with time

increased circulating cholesterol level ( J:191046 )

• moderate increase at 4 weeks of age that does not vary much with time

increased circulating triglyceride level ( J:191046 )

• plasma levels are increased 30- to 100-fold from 4 to 8 weeks of age

decreased circulating total protein level ( J:191046 )

ascites ( J:191046 )

• fluid contains albumin and beta globins at similar levels to blood plasma but sharply reduced alpha fractions

growth/size/body

postnatal growth retardation ( J:191046 )

distended abdomen ( J:191046 )

• due to accumulation of ascites

adipose tissue

decreased white adipose tissue amount ( J:191046 )

endocrine/exocrine glands

small pancreas ( J:191046 )

Genotype
MGI:7537042

cn4

• Allelic Composition: Best3^tm1.1Zhoj/Best3^tm1.1Zhoj; Tg(Cdh5-cre)1Spe/0
• Genetic Background: involves: 129S1/SvImJ * C57BL/6J

cardiovascular system

cardiovascular system phenotype ( J:338981 )

N • no effect on development of aortic dissections or change in blood pressure