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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Aurkatm1.1Tvd
targeted mutation 1.1, Terry Van Dyke
MGI:3836420
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Aurkatm1.1Tvd/Aurkatm1.1Tvd
Gt(ROSA)26Sortm1(cre/ERT)Nat/Gt(ROSA)26Sortm1Sor 		involves: 129 * 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J MGI:3836424
cn2
 		Aurkatm1.1Tvd/Aurkatm1.1Tvd
Gt(ROSA)26Sortm1(cre/ERT)Nat/Gt(ROSA)26Sor+ 		involves: 129 * 129P2/OlaHsd * C57BL/6J MGI:3836425
cn3
 		Aurkatm1.1Tvd/Aurkatm1.1Tvd
Ccn6tm1(cre)Mawa/Ccn6+ 		involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6J MGI:7287844
cn4
 		Aurkatm1.1Tvd/Aurkatm1.1Tvd
Tg(Ddx4-cre)1Dcas/0 		involves: 129P2/OlaHsd * C57BL/6J * FVB MGI:7287842


Genotype
MGI:3836424
cn1
Allelic
Composition		 Aurkatm1.1Tvd/Aurkatm1.1Tvd
Gt(ROSA)26Sortm1(cre/ERT)Nat/Gt(ROSA)26Sortm1Sor
Genetic
Background		 involves: 129 * 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aurkatm1.1Tvd mutation (1 available); any Aurka mutation (40 available)
Gt(ROSA)26Sortm1(cre/ERT)Nat mutation (2 available); any Gt(ROSA)26Sor mutation (991 available)
Gt(ROSA)26Sortm1Sor mutation (7 available); any Gt(ROSA)26Sor mutation (991 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal cell nucleus morphology ( J:145744 )
• in MEFs at later time points after OHT treatment and serum addition cells develop large aberrant nuclei with frequent micronuclei
abnormal chromosome number ( J:145744 )
• in MEFS after OHT treatment and serum addition increases in the population of cells with 4N and 8N DNA content are seen
abnormal mitosis ( J:145744 )
• live cell imaging indicates a median 8 h delay in mitotic entry in MEFS after OHT treatment and serum addition compared to controls
• in MEFS after OHT treatment and serum addition more cells are in prophase and most of these show an early prophase phenotype
• in MEFS after OHT treatment and serum addition almost no cells in metaphase, anaphase, and telophase are detected
• in MEFS after OHT treatment and serum addition cells in prometaphase have a monopolar spindle with closely adjacent chromosomes, the presence of these monopolar spindles activates the spindle checkpoint
increased mitotic index ( J:145744 )
• in MEFS 28 h after OHT treatment and serum addition the percent of cells positive for PH3 is increased about 3 fold compared to controls
• however, the timing of the peak in PH3 positive cells is similar to controls
decreased cell proliferation ( J:145744 )
• growth defect in MEFs following OHT treatment and serum addition




Genotype
MGI:3836425
cn2
Allelic
Composition		 Aurkatm1.1Tvd/Aurkatm1.1Tvd
Gt(ROSA)26Sortm1(cre/ERT)Nat/Gt(ROSA)26Sor+
Genetic
Background		 involves: 129 * 129P2/OlaHsd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aurkatm1.1Tvd mutation (1 available); any Aurka mutation (40 available)
Gt(ROSA)26Sortm1(cre/ERT)Nat mutation (2 available); any Gt(ROSA)26Sor mutation (991 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal mitosis ( J:145744 )
• following injection of tamoxifen at E10.5 most embryonic cells appear to be in prometaphase
increased mitotic index ( J:145744 )
• following injection of tamoxifen at E10.5 embryos show a 4 fold increase in PH3 positive cells in the thymus and lungs compared to controls
increased apoptosis ( J:145744 )
• following injection of tamoxifen at E10.5 about a 3 fold increase in the percentage of apoptotic cells in the thymus and lungs is seen




Genotype
MGI:7287844
cn3
Allelic
Composition		 Aurkatm1.1Tvd/Aurkatm1.1Tvd
Ccn6tm1(cre)Mawa/Ccn6+
Genetic
Background		 involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aurkatm1.1Tvd mutation (1 available); any Aurka mutation (40 available)
Ccn6tm1(cre)Mawa mutation (1 available); any Ccn6 mutation (23 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
oligozoospermia ( J:321647 )
• males show a ~5-fold reduction in cauda epididymal sperm number relative to control males
teratozoospermia ( J:321647 )
• males show a ~3-fold increase in morphologically abnormal cauda epididymidal sperm relative to control males
abnormal sperm midpiece morphology ( J:321647 )
• 63% of sperm show midpiece curling
kinked sperm flagellum ( J:321647 )
• 29% of sperm have bent tails, with flagella bent or folded at the annulus
multiflagellated sperm ( J:321647 )
• 2% of sperm have multiple tails
abnormal sperm head morphology ( J:321647 )
• 6% of sperm show head malformations
increased male germ cell apoptosis ( J:321647 )
• males show a 3-fold increase in spermatocyte apoptosis, as determined by co-staining with cleaved cPARP1 (a marker for apoptosis) and SYCP3 (a spermatocyte marker)
small testis ( J:321647 )
• adult testes are ~30% smaller than control testes
• however, testis histology shows complete spermatogenesis with supporting cells and multiple generations of germ cells
decreased testis weight ( J:321647 )
• adult testis weight is significantly lower than in control males
• however, adult body weight is normal
decreased litter size ( J:321647 )
• when adult males are bred with wild-type females for 4 months, average litter size is significantly lower than in control males
• however, total number of litters is normal
abnormal sperm physiology ( J:321647 )
• sperm lysates exhibit significantly reduced PPP1CC (protein phosphatase 1 catalytic subunit gamma, aka PP1) activity relative to lysates from control sperm
abnormal sperm motility ( J:321647 )
• specific sperm motility parameters, including % progressive motility, path velocity (VAP), curvilinear velocity (VCL) and relative motility index (RMI = % motility x straight line velocity (VSL)/100), are all significantly increased relative to controls
• however, % total motility and other parameters including lateral amplitude (ALH), beat frequency (BCF), straightness (STR), and linearity (LIN) are not significantly altered
increased sperm progressive motility ( J:321647 )
• sperm progressive motility is increased by 25% relative to that in control males

cellular
oligozoospermia ( J:321647 )
• males show a ~5-fold reduction in cauda epididymal sperm number relative to control males
teratozoospermia ( J:321647 )
• males show a ~3-fold increase in morphologically abnormal cauda epididymidal sperm relative to control males
abnormal sperm midpiece morphology ( J:321647 )
• 63% of sperm show midpiece curling
kinked sperm flagellum ( J:321647 )
• 29% of sperm have bent tails, with flagella bent or folded at the annulus
multiflagellated sperm ( J:321647 )
• 2% of sperm have multiple tails
abnormal sperm head morphology ( J:321647 )
• 6% of sperm show head malformations
increased male germ cell apoptosis ( J:321647 )
• males show a 3-fold increase in spermatocyte apoptosis, as determined by co-staining with cleaved cPARP1 (a marker for apoptosis) and SYCP3 (a spermatocyte marker)
abnormal sperm motility ( J:321647 )
• specific sperm motility parameters, including % progressive motility, path velocity (VAP), curvilinear velocity (VCL) and relative motility index (RMI = % motility x straight line velocity (VSL)/100), are all significantly increased relative to controls
• however, % total motility and other parameters including lateral amplitude (ALH), beat frequency (BCF), straightness (STR), and linearity (LIN) are not significantly altered
increased sperm progressive motility ( J:321647 )
• sperm progressive motility is increased by 25% relative to that in control males

endocrine/exocrine glands
small testis ( J:321647 )
• adult testes are ~30% smaller than control testes
• however, testis histology shows complete spermatogenesis with supporting cells and multiple generations of germ cells
decreased testis weight ( J:321647 )
• adult testis weight is significantly lower than in control males
• however, adult body weight is normal




Genotype
MGI:7287842
cn4
Allelic
Composition		 Aurkatm1.1Tvd/Aurkatm1.1Tvd
Tg(Ddx4-cre)1Dcas/0
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6J * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aurkatm1.1Tvd mutation (1 available); any Aurka mutation (40 available)
Tg(Ddx4-cre)1Dcas mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
azoospermia ( J:321647 )
• cauda epididymides are devoid of sperm
absent germ cells ( J:321647 )
• males show absence of all developing germ cells in the testis, presumably due to a block in mitosis
abnormal seminiferous tubule morphology ( J:321647 )
• seminiferous tubules lack germ cells and exhibit Sertoli cell-only phenotype
abnormal seminiferous tubule epithelium morphology ( J:321647 )
• spermatogonia are eliminated, only Sertoli cells are detected in the seminiferous epithelium
small testis ( J:321647 )
• testes are significantly smaller than those in control males
abnormal cauda epididymis morphology ( J:321647 )
• cauda epididymides are devoid of sperm
• however, cauda epididymis weight is normal

cellular
azoospermia ( J:321647 )
• cauda epididymides are devoid of sperm
absent germ cells ( J:321647 )
• males show absence of all developing germ cells in the testis, presumably due to a block in mitosis

endocrine/exocrine glands
abnormal seminiferous tubule morphology ( J:321647 )
• seminiferous tubules lack germ cells and exhibit Sertoli cell-only phenotype
abnormal seminiferous tubule epithelium morphology ( J:321647 )
• spermatogonia are eliminated, only Sertoli cells are detected in the seminiferous epithelium
small testis ( J:321647 )
• testes are significantly smaller than those in control males




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Send questions and comments to User Support. 		last database update
10/09/2024
MGI 6.24 		The Jackson Laboratory