Phenotypes associated with this allele

• Allele Symbol: Numa1^tm1.1Dwc
• Allele Name: targeted mutation 1.1, Don W Cleveland
• Allele ID: MGI:3838102
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Numa1^tm1.1Dwc/Numa1^tm1.1Dwc	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:3838128
cn2	Numa1^tm1.1Dwc/Numa1^tm1.1Dwc Tg(CAG-cre/Esr1*)5Amc/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL	MGI:3838126
cn3	Numa1^tm1.1Dwc/Numa1^tm1.1Dwc Tg(SLC18A3-cre)Misa/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:3838129

Genotype
MGI:3838128

hm1

• Allelic Composition: Numa1^tm1.1Dwc/Numa1^tm1.1Dwc
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:146214 )

N • mice exhibit normal Mendelian ratios at P21

Genotype
MGI:3838126

cn2

• Allelic Composition: Numa1^tm1.1Dwc/Numa1^tm1.1Dwc; Tg(CAG-cre/Esr1*)5Amc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL

Spindle defects in primay Numa1^tm1.1Dwc/Numa1^tm1.1Dwc Tg(CAG-cre/Esr1*)5Amc/0 fibroblasts

cellular

cellular phenotype ( J:146214 )

N • despite spindle defects, bulk chromosome segregation is largely normal

abnormal mitotic spindle morphology ( J:146214 )

• tamoxifen-treated mouse embryonic fibroblasts (MEFs) exhibit detachment of microtubule-nucleating structures from the ends of the mitotic spindles unlike wild-type cells

• 50% of metaphase-like tamoxifen-treated MEFs have at least one centrosome that is not clearly associated with a spindle pole unlike in wild-type cells

• tension between sister kinetochores in pole-focused spindles in tamoxifen-treated MEFs exposed to MG132 is 33% less than in wild-type cells

• more like tamoxifen-treated MEFs exhibit pole defocusing and centrosome detachment than in wild-type cells

absent fibroblast proliferation ( J:146214 )

• tamoxifen-treated mouse embryonic fibroblasts (MEFs) fail to increase in number after release from growth arrest unlike wild-type cells

Genotype
MGI:3838129

cn3

• Allelic Composition: Numa1^tm1.1Dwc/Numa1^tm1.1Dwc; Tg(SLC18A3-cre)Misa/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

behavior/neurological

behavior/neurological phenotype ( J:146214 )

N • mice exhibit normal motor neuron behaviors

nervous system

nervous system phenotype ( J:146214 )

N • motor neuron numbers are normal