Phenotypes associated with this allele

• Allele Symbol: Pou4f2^tm4(DTA)Whk
• Allele Name: targeted mutation 4, William H Klein
• Allele ID: MGI:3838536
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Opn4^tm1.1(cre)Saha/Opn4^+ Pou4f2^tm4(DTA)Whk/Pou4f2^+ Tg(CAG-Bgeo/ALPP)1Lbe/0	involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5285913
cn2	Opn4^tm1.1(cre)Saha/Opn4^+ Pou4f2^tm4(DTA)Whk/Pou4f2^+	involves: 129S/SvEv * C57BL/6	MGI:5285914
cn3	Opn4^tm1.1(cre)Saha/Opn4^tm1Yau Pou4f2^tm4(DTA)Whk/Pou4f2^+	involves: 129S/SvEv * C57BL/6	MGI:5285915
cn4	Pou4f2^tm4(DTA)Whk/Pou4f2^+ Tg(CAG-cre/Esr1*)5Amc/0	involves: 129S/SvEv * C57BL/6 * CBA	MGI:3838797
cn5	Pou4f2^tm4(DTA)Whk/Pou4f2^tm4(DTA)Whk Tg(Six3-cre)69Frty/?	involves: 129S/SvEv * C57BL/6 * DBA/2	MGI:3838794

Genotype
MGI:5285913

cn1

• Allelic Composition: Opn4^{tm1.1(cre)Saha}/Opn4⁺; Pou4f2^tm4(DTA)Whk/Pou4f2⁺; Tg(CAG-Bgeo/ALPP)1Lbe/0
• Genetic Background: involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal olivary pretectal nucleus morphology ( J:174940 )

• and olivary pretectal nucleus (OPN) projections are eliminated, compared to controls

abnormal innervation ( J:174940 )

• innervation of the intergeniculate leaflet (IGL) is markedly reduced

decreased retina ganglion cell number ( J:174940 )

• all non-M1 retinal ganglion cells are ablated in the retinas, with only about 200 Pou4f2-negative cells detected (200/2000); these remaining fibers innervated the SCN to the same extent as control Pou4f2^tm2.1Nat/+ Opn4^{tm1.1(cre)Saha/+} animals

vision/eye

decreased retina ganglion cell number ( J:174940 )

• all non-M1 retinal ganglion cells are ablated in the retinas, with only about 200 Pou4f2-negative cells detected (200/2000); these remaining fibers innervated the SCN to the same extent as control Pou4f2^tm2.1Nat/+ Opn4^{tm1.1(cre)Saha/+} animals

Genotype
MGI:5285914

cn2

• Allelic Composition: Opn4^{tm1.1(cre)Saha}/Opn4⁺; Pou4f2^tm4(DTA)Whk/Pou4f2⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6

behavior/neurological

behavior/neurological phenotype ( J:174940 )

N • mutants are able to photoentrain (their circadian phase) like controls; also, activity

impaired pupillary reflex ( J:174940 )

• the papillary light reflex (PLR) is significantly attenuated at high and low light intensities in the middle of the day (zeitgerber time 8, ZT8); in the middle of the night (ZT20), mice show no PLR

abnormal circadian behavior period ( J:174940 )

• animals show a minor deficit in period lengthening under constant light conditions

nervous system

decreased retina ganglion cell number ( J:174940 )

• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas

vision/eye

vision/eye phenotype ( J:174940 )

N • visual acuity is comparable to wild-type animals

impaired pupillary reflex ( J:174940 )

• the papillary light reflex (PLR) is significantly attenuated at high and low light intensities in the middle of the day (zeitgerber time 8, ZT8); in the middle of the night (ZT20), mice show no PLR

decreased retina ganglion cell number ( J:174940 )

• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas

Genotype
MGI:5285915

cn3

• Allelic Composition: Opn4^{tm1.1(cre)Saha}/Opn4^tm1Yau; Pou4f2^tm4(DTA)Whk/Pou4f2⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6

nervous system

abnormal olivary pretectal nucleus morphology ( J:174940 )

• and olivary pretectal nucleus (OPN) projections are eliminated, compared to controls

• no fibres are observed in the shell of the OPN

abnormal innervation ( J:174940 )

• innervation of the intergeniculate nucleus (IGL) is markedly reduced

decreased retina ganglion cell number ( J:174940 )

• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas; these remaining fibers innervated the SCN to the same extent as control Pou4f2^tm2.1Nat/+ ;Opn4^{tm1.1(cre)Saha/+} animals

vision/eye

decreased retina ganglion cell number ( J:174940 )

• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas; these remaining fibers innervated the SCN to the same extent as control Pou4f2^tm2.1Nat/+ ;Opn4^{tm1.1(cre)Saha/+} animals

Genotype
MGI:3838797

cn4

• Allelic Composition: Pou4f2^tm4(DTA)Whk/Pou4f2⁺; Tg(CAG-cre/Esr1*)5Amc/0
• Genetic Background: involves: 129S/SvEv * C57BL/6 * CBA

vision/eye

abnormal optic nerve morphology ( J:146558 )

• reduced cross-sectional area by 48.8% in 3 months after a 5 day course of tamoxifen treatment

• cross-sectional area reduced to 19.7% of controls at ten months after a 5 day course of tamoxifen treatment

• axons disorganized

optic nerve degeneration ( J:146558 )

• progressive degeneration over the course of ten months after a 5 day course of tamoxifen treatment

• severe axon degeneration and vacuolation

• empty or abnormal myelin sheaths

optic nerve atrophy ( J:146558 )

thin retina ganglion layer ( J:146558 )

• 60% reduction in the number of cells of the ganglion layer one month after a 5 day course of tamoxifen treatment

abnormal vision ( J:146558 )

• 33% of time spent in the light as opposed to 6% for controls after 1-2 hours of dark adaptation

nervous system

gliosis ( J:146558 )

• reactive gliosis triggered one month after a 5 day course of tamoxifen treatment

abnormal optic nerve morphology ( J:146558 )

• reduced cross-sectional area by 48.8% in 3 months after a 5 day course of tamoxifen treatment

• cross-sectional area reduced to 19.7% of controls at ten months after a 5 day course of tamoxifen treatment

• axons disorganized

optic nerve degeneration ( J:146558 )

• progressive degeneration over the course of ten months after a 5 day course of tamoxifen treatment

• severe axon degeneration and vacuolation

• empty or abnormal myelin sheaths

optic nerve atrophy ( J:146558 )

Genotype
MGI:3838794

cn5

• Allelic Composition: Pou4f2^tm4(DTA)Whk/Pou4f2^tm4(DTA)Whk; Tg(Six3-cre)69Frty/?
• Genetic Background: involves: 129S/SvEv * C57BL/6 * DBA/2

vision/eye

abnormal optic nerve morphology ( J:97089 )

• clearly abnormal in 16 day old mice

• thinner with smaller diameter fibers

• unmyelinated fibers

abnormal optic disk morphology ( J:97089 )

• deformed at E14.5

abnormal retina morphology ( J:97089 )

• overall structure remains normal through 5 months of age

• reduced cell proliferation and increased apoptosis

thin retina ganglion layer ( J:97089 )

• more than 98% of retinal ganglion cells ablated at E14.5

• only displaced amacrine cells present at 16 days of age

decreased total retina thickness ( J:97089 )

• noticeably thinner at E14.5

• 30-50% thinner than controls at 16 days of age

abnormal eye electrophysiology ( J:97089 )

• saturated amplitudes of both light and dark adapted b-waves 25% of normal

• dark adapted a-wave amplitude 42% of normal

• negative scotopic threshold response is very small

• positive scotopic threshold response is absent

nervous system

abnormal optic nerve morphology ( J:97089 )

• clearly abnormal in 16 day old mice

• thinner with smaller diameter fibers

• unmyelinated fibers

abnormal optic disk morphology ( J:97089 )

• deformed at E14.5