Phenotypes associated with this allele

• Allele Symbol: Tg(Gfap-cre)77.6Mvs
• Allele Name: transgene insertion 77.6, Michael V Sofroniew
• Allele ID: MGI:3838840
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Chrna7^tm1.1Ehs/Chrna7^tm1.1Ehs Tg(Gfap-cre)77.6Mvs/0	B6.Cg-Chrna7^tm1.1Ehs Tg(Gfap-cre)77.6Mvs	MGI:5620296
cn2	Kras^tm4Tyj/Kras^+ Tg(Gfap-cre)77.6Mvs/0	involves: 129 * BALB/c * C57BL/6NHsd	MGI:4849444
cn3	Nf1^tm1Par/Nf1^+ Pten^tm1Hwu/Pten^+ Tg(Gfap-cre)77.6Mvs/0	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * BALB/c * C57BL/6NHsd	MGI:4849443
cn4	Nf1^tm1Par/Nf1^tm1Par Tg(Gfap-cre)77.6Mvs/0	involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6NHsd	MGI:4849442
cn5	Pten^tm1Hwu/Pten^tm1Hwu Tg(Gfap-cre)77.6Mvs/0	involves: 129S4/SvJae * BALB/c * C57BL/6NHsd	MGI:3838843
cn6	Kras^tm4Tyj/Kras^+ Pten^tm1Hwu/Pten^+ Tg(Gfap-cre)77.6Mvs/0	involves: 129S4/SvJae * BALB/c * C57BL/6NHsd	MGI:4849441
cn7	Prrc2a^tm1Fcw/Prrc2a^tm1Fcw Tg(Gfap-cre)77.6Mvs/0	involves: BALB/c * C57BL/6NHsd	MGI:6724443
cn8	Acsl6^tm1Jeme/Acsl6^tm1Jeme Tg(Gfap-cre)77.6Mvs/0	involves: BALB/cJ * C57BL/6	MGI:6269169

Genotype
MGI:5620296

cn1

• Allelic Composition: Chrna7^tm1.1Ehs/Chrna7^tm1.1Ehs; Tg(Gfap-cre)77.6Mvs/0
• Genetic Background: B6.Cg-Chrna7^tm1.1Ehs Tg(Gfap-cre)77.6Mvs

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:219327 )

• mice are viable and fertile and exhibit no altered behavioral, cognitive, or electrophysiological properties

Genotype
MGI:4849444

cn2

• Allelic Composition: Kras^tm4Tyj/Kras⁺; Tg(Gfap-cre)77.6Mvs/0
• Genetic Background: involves: 129 * BALB/c * C57BL/6NHsd

neoplasm

neoplasm ( J:154673 )

N • mutants do not develop tumors

Genotype
MGI:4849443

cn3

• Allelic Composition: Nf1^tm1Par/Nf1⁺; Pten^tm1Hwu/Pten⁺; Tg(Gfap-cre)77.6Mvs/0
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * BALB/c * C57BL/6NHsd

neoplasm

neoplasm ( J:154673 )

N • mutants do not develop tumors

Genotype
MGI:4849442

cn4

• Allelic Composition: Nf1^tm1Par/Nf1^tm1Par; Tg(Gfap-cre)77.6Mvs/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6NHsd

neoplasm

neoplasm ( J:154673 )

N • mutants do not develop tumors

Genotype
MGI:3838843

cn5

• Allelic Composition: Pten^tm1Hwu/Pten^tm1Hwu; Tg(Gfap-cre)77.6Mvs/0
• Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6NHsd

nervous system

abnormal neuron differentiation ( J:146630 )

• cultured adult neural stem cells maintain their ability to differentiate into neurons for a longer time compared to wild-type cells

abnormal neuronal precursor proliferation ( J:146630 )

• in culture, neurospheres formed by adult neural stem cells are generally larger and the stem cells' capacity for self renewal is prolonged compared to wild-type cells

abnormal response to CNS ischemic injury ( J:146630 )

• 7 days after induction of limited stroke in the sensorimotor cortex, mice have more neuroblasts in the peri-infarct cortex compared to controls

• however, there is no difference in the number of new cells present at 90 days after stroke induction

abnormal olfactory bulb morphology ( J:146630 )

• continuous increase in the weight and size of the olfactory bulb compared to controls starting at 2.5 months of age

• the granule cell layer volume is increased 2 fold at 3.5 months of age

• the increase in volume is due to an increase in the number of cells migrating from the subependymal zone to the olfactory bulb and a decrease in the number of apoptotic cells in the granule cell layer

abnormal postnatal subventricular zone morphology ( J:146630 )

• increase in the volume of the subependymal zone compared to controls

• increase in the number of proliferating cells and DCX positive neuroblasts

increased neuronal precursor cell number ( J:146630 )

• expansion of the adult neural stem cell and progenitor populations in the subependymal zone

behavior/neurological

abnormal response to novel odor ( J:146630 )

• habituate faster to a novel odorant compared to controls

• however, no difference in response is seen on the first exposure to a novel odorant

taste/olfaction

abnormal olfaction ( J:146630 )

• mice recover olfactory ability more quickly following exposure to dichlobenil compared to controls

neoplasm

neoplasm ( J:146630 , J:154673 )

N • in contrast to other conditional null Pten genotypes no tumors are found in mice up to 2 years of age (J:146630)

N (J:154673)

homeostasis/metabolism

abnormal response to CNS ischemic injury ( J:146630 )

• 7 days after induction of limited stroke in the sensorimotor cortex, mice have more neuroblasts in the peri-infarct cortex compared to controls

• however, there is no difference in the number of new cells present at 90 days after stroke induction

cellular

abnormal neuron differentiation ( J:146630 )

• cultured adult neural stem cells maintain their ability to differentiate into neurons for a longer time compared to wild-type cells

abnormal neuronal precursor proliferation ( J:146630 )

• in culture, neurospheres formed by adult neural stem cells are generally larger and the stem cells' capacity for self renewal is prolonged compared to wild-type cells

Genotype
MGI:4849441

cn6

• Allelic Composition: Kras^tm4Tyj/Kras⁺; Pten^tm1Hwu/Pten⁺; Tg(Gfap-cre)77.6Mvs/0
• Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6NHsd

mortality/aging

premature death ( J:154673 )

• survival is on average 22 weeks

neoplasm

increased classified tumor incidence ( J:154673 )

• mutants develop multiple visible subcutaneous tumors with 100% penetrance, starting from 4 months of age

• majority of tumors are located on the back and sides

increased neurofibroma incidence ( J:154673 )

• each mutant has more than one lesion with features of human neurofibroma and malignant peripheral nerve sheath tumor

increased neurofibrosarcoma incidence ( J:154673 )

• progressive development of malignant peripheral nerve sheath tumors (MPNST) from neurofibroma, with 100% of mutants showing MPNST when followed for 7 months

• Pten loss of heterozygosity in mutants correlates with MPNST transformation from neurofibromas

nervous system

increased neurofibroma incidence ( J:154673 )

• each mutant has more than one lesion with features of human neurofibroma and malignant peripheral nerve sheath tumor

increased neurofibrosarcoma incidence ( J:154673 )

• progressive development of malignant peripheral nerve sheath tumors (MPNST) from neurofibroma, with 100% of mutants showing MPNST when followed for 7 months

• Pten loss of heterozygosity in mutants correlates with MPNST transformation from neurofibromas

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
malignant peripheral nerve sheath tumor		DOID:5940		J:154673
neurofibromatosis 1		DOID:0111253	OMIM:162200	J:154673

Genotype
MGI:6724443

cn7

• Allelic Composition: Prrc2a^tm1Fcw/Prrc2a^tm1Fcw; Tg(Gfap-cre)77.6Mvs/0
• Genetic Background: involves: BALB/c * C57BL/6NHsd

nervous system

nervous system phenotype ( J:301796 )

N • mice exhibit normal myelination and oligodendrocyte and astrocyte populations

Genotype
MGI:6269169

cn8

• Allelic Composition: Acsl6^tm1Jeme/Acsl6^tm1Jeme; Tg(Gfap-cre)77.6Mvs/0
• Genetic Background: involves: BALB/cJ * C57BL/6

homeostasis/metabolism

decreased lipogenesis ( J:267563 )

• decreased omega-3 docosahexaenoic acid (DHA) levels in brain

• decreased omega-6 Arachidonate-containing phospholipids level in brain

• 57% decreased Acyl-CoA synthetase (ACS) in cerebellum