Phenotypes associated with this allele

• Allele Symbol: Tg(HCRT-cre)1Stak
• Allele Name: transgene insertion 1, Takeshi Sakurai
• Allele ID: MGI:3839422
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Hap1^tm2Xjl/Hap1^tm2Xjl Tg(HCRT-cre)1Stak/0	involves: 129 * C57BL/6 * DBA/1	MGI:4830318
cn2	Gabbr1^tm2Bet/Gabbr1^tm2Bet Tg(HCRT-cre)1Stak/0	involves: BALB/cA * C57BL/6 * DBA/1	MGI:3839426

Genotype
MGI:4830318

cn1

• Allelic Composition: Hap1^tm2Xjl/Hap1^tm2Xjl; Tg(HCRT-cre)1Stak/0
• Genetic Background: involves: 129 * C57BL/6 * DBA/1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Reduced body weight in Hap1^tm2Xjl/Hap1^tm2Xjl Tg(HCRT-cre)1Stak/0 mice

growth/size/body

decreased body weight ( J:163896 )

• the greatest differences are seen at the ages of 2?4 weeks

behavior/neurological

decreased food intake ( J:163896 )

• males and females consume 66 and 50%, respectively, of the food that control mice eat

• however, water consumption is normal

decreased locomotor activity ( J:163896 )

• show less activity than the control mice at night, with females showing a greater difference

• show less activity in response to fasting-induced metabolic stress

nervous system

abnormal hypothalamus morphology ( J:163896 )

abnormal neurite morphology ( J:163896 )

• orexin neurons of the brain display disrupted or fragmented neurites in the dorsomedial hypothalamic and lateral hypothalamic nuclei

Genotype
MGI:3839426

cn2

• Allelic Composition: Gabbr1^tm2Bet/Gabbr1^tm2Bet; Tg(HCRT-cre)1Stak/0
• Genetic Background: involves: BALB/cA * C57BL/6 * DBA/1

nervous system

nervous system phenotype ( J:146778 )

N • despite loss of Gabbr1 expression, no decrease in the number of orexin positive neurons is detected and spontaneous excitatory postsynaptic currents in orexin neurons are similar to controls

abnormal CNS synaptic transmission ( J:146778 )

• unlike in controls, treatment with bicuculline induces depolarization of orexin neurons

• membrane resistance of orexin neurons is significantly decreased

• glutamate and GABA-A agonists are less potent in inducing depolarizing currents in orexin neurons compared to controls

abnormal inhibitory postsynaptic currents ( J:146778 )

• increase in the frequency of fast spontaneous inhibitory postsynaptic currents in orexin neurons compared to control littermates

behavior/neurological

abnormal sleep pattern ( J:146778 )

• severe fragmentation of the sleep wake cycle with decreased awake, non-REM, and REM sleep episode durations and reduced REM latency

• fragmentation occurs in both the light and dark phases

• the delta power is significantly increased in non-REM sleep and the theta power is decreased during REM sleep compared to controls

• however, the overall amounts of wakefulness, non-REM and REM sleep are similar to controls