Phenotypes associated with this allele

• Allele Symbol: Mre11a^tm1Dof
• Allele Name: targeted mutation 1, David O Ferguson
• Allele ID: MGI:3839938
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mre11a^tm1Dof/Mre11a^tm1Dof	involves: 129S6/SvEvTac	MGI:3839951
cn2	Mre11a^tm1Dof/Mre11a^tm2.1Dof	involves: 129S6/SvEvTac	MGI:3839952

Genotype
MGI:3839951

hm1

• Allelic Composition: Mre11a^tm1Dof/Mre11a^tm1Dof
• Genetic Background: involves: 129S6/SvEvTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:147247 )

• although present at E9.5, no mice are recovered at E10.5

embryo

abnormal embryo development ( J:147247 )

• at E9.5 and E7.5, embryos are disorganized with a poorly defined axis unlike in wild-type mice

embryonic growth arrest ( J:147247 )

• at E9.5 and E7.5

cardiovascular system

abnormal heart development ( J:147247 )

• at E9.5, mice exhibit no gross evidence of heart development unlike wild-type mice

Genotype
MGI:3839952

cn2

• Allelic Composition: Mre11a^tm1Dof/Mre11a^tm2.1Dof
• Genetic Background: involves: 129S6/SvEvTac

cellular

cellular phenotype ( J:147247 )

N • cre-treated mouse embryonic fibroblasts exhibit normal G2/M checkpoint activation following exposure to ionizing radiation

increased cellular sensitivity to ionizing radiation ( J:147247 )

• cre-treated mouse embryonic fibroblasts exhibit increased cellular sensitivity to ionizing radiation compared to similarly treated heterozygous cells that is not as severe as in Lig4^tm1Fwa cells

• cre-treated mouse embryonic fibroblasts exhibit defective DNA repair following treatment with ionizing radiation compared to in similarly treated heterozygous mice

decreased cell proliferation ( J:147247 )

• cre-exposed mouse embryonic fibroblasts exhibit decreased growth rates by the second passage and no increase in numbers beyond passage three unlike similarly treated heterozygous cells

abnormal DNA repair ( J:147247 )

• cre-treated mouse embryonic fibroblasts exhibit defective DNA repair following treatment with ionizing radiation compared to in wild-type mice

chromosomal instability ( J:147247 )

• cre-exposed mouse embryonic fibroblasts (MEFs) exhibit chromosomal instability including chromosome or chromatid fragments, chromosome breaks or gaps in a single chromatid, occasional radial structures, and dicentrics unlike in similarly treated heterozygous cells

• following exposure to gamma radiation, cre-exposed MEFs fail to exhibit a downward trend in frequency of chromosomal anomalies unlike similarly treated heterozygous cells

induced chromosome breakage ( J:147247 )

• cre-treated mouse embryonic fibroblasts exhibit hypersensitivity to aphidicolin compared to similarly treated heterozygous cells

homeostasis/metabolism

abnormal DNA repair ( J:147247 )

• cre-treated mouse embryonic fibroblasts exhibit defective DNA repair following treatment with ionizing radiation compared to in wild-type mice