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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Foxo1tm1Flv
targeted mutation 1, Richard A Flavell
MGI:3840910
Summary 7 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Foxo1tm1Flv/Foxo1tm1Flv
Ndor1Tg(UBC-cre/ERT2)1Ejb/0
involves: 129S/SvEv * 129S6/SvEvTac * C57BL/6 MGI:5448154
cn2
Foxo1tm1Flv/Foxo1tm1Flv
Ifngtm1Ts/Ifngtm1Ts
Foxp3tm4(YFP/icre)Ayr/Foxp3+
involves: 129S1/Sv * 129S6/SvEvTac * 129S7/SvEvBrd * 129X1/SvJ MGI:5448156
cn3
Foxo1tm1Flv/Foxo1tm1Flv
Foxp3tm4(YFP/icre)Ayr/Foxp3+
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ MGI:5448157
cn4
Foxo1tm1Flv/Foxo1tm1Flv
Gt(ROSA)26Sortm1(CAG-FOXO1,GFP)Moli/Gt(ROSA)26Sor+
Foxp3tm4(YFP/icre)Ayr/Foxp3+
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 MGI:5448151
cn5
Foxo1tm1Flv/Foxo1tm1Flv
Rag1tm1Mom/Rag1tm1Mom
Tg(Cd4-cre)1Cwi/?
Tg(TcraTcrb)425Cbn/?
involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * DBA/2 MGI:3840970
cn6
Foxo1tm1Flv/Foxo1tm1Flv
Rag1tm1Mom/Rag1tm1Mom
Tg(CD2-Il7r)1Asin/?
Tg(Cd4-cre)1Cwi/?
Tg(TcraTcrb)425Cbn/?
involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * DBA/2 MGI:3840971
cn7
Foxo1tm1Flv/Foxo1tm1Flv
Tg(Cd4-cre)1Cwi/?
involves: 129S6/SvEvTac * C57BL/6 * DBA/2 MGI:3840969


Genotype
MGI:5448154
cn1
Allelic
Composition
Foxo1tm1Flv/Foxo1tm1Flv
Ndor1Tg(UBC-cre/ERT2)1Ejb/0
Genetic
Background
involves: 129S/SvEv * 129S6/SvEvTac * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Ndor1Tg(UBC-cre/ERT2)1Ejb mutation (6 available); any Ndor1 mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• regulatory T cells from tamoxifen-treated mice under the condition of IL12 and IFN-gamma stimulation unlike wild-type cells
• however, production in CD4+ T cells is normal




Genotype
MGI:5448156
cn2
Allelic
Composition
Foxo1tm1Flv/Foxo1tm1Flv
Ifngtm1Ts/Ifngtm1Ts
Foxp3tm4(YFP/icre)Ayr/Foxp3+
Genetic
Background
involves: 129S1/Sv * 129S6/SvEvTac * 129S7/SvEvBrd * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Foxp3tm4(YFP/icre)Ayr mutation (2 available); any Foxp3 mutation (58 available)
Ifngtm1Ts mutation (18 available); any Ifng mutation (49 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• the lethal inflammatory phenotype is partially rescued

immune system
N
• regulatory T cells are able to suppress development of colitis in Rag1 null mice receiving naive T cells
• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1tm1Flv/Foxo1tm1Flv Foxp3tm4(YFP/cre)Ayr/Foxp3+ mice
• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1tm1Flv/Foxo1tm1Flv Foxp3tm4(YFP/cre)Ayr/Foxp3+ mice

hematopoietic system
• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1tm1Flv/Foxo1tm1Flv Foxp3tm4(YFP/cre)Ayr/Foxp3+ mice
• elevated numbers in the spleen and peripheral lymph nodes are partially rescued compared to in Foxo1tm1Flv/Foxo1tm1Flv Foxp3tm4(YFP/cre)Ayr/Foxp3+ mice




Genotype
MGI:5448157
cn3
Allelic
Composition
Foxo1tm1Flv/Foxo1tm1Flv
Foxp3tm4(YFP/icre)Ayr/Foxp3+
Genetic
Background
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Foxp3tm4(YFP/icre)Ayr mutation (2 available); any Foxp3 mutation (58 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• moribund within 35 days of birth

immune system
• in the spleen and peripheral lymph node
• in the spleen and peripheral lymph nodes
• in the spleen and peripheral lymph nodes
• in peripheral lymph node at day 20
• however, thymic and splenic regulatory T cell numbers are normal at day 12
• regulatory T cells are not able to suppress development of colitis in Rag1 null mice receiving na[?]ve T cells unlike wild-type regulatory T cells
• infiltrate of leukocytes in the salivary glands, lung interstitia, liver sinusoids, pancreas acini, stomach and colon mucosa

behavior/neurological
• lack of mobility associated with hunched posture
• lack of mobility associated with hunched posture

integument
• crusting of the ears, eyelids and tail

limbs/digits/tail
• scurfy tail

hematopoietic system
• in the spleen and peripheral lymph node
• in the spleen and peripheral lymph nodes
• in the spleen and peripheral lymph nodes
• in peripheral lymph node at day 20
• however, thymic and splenic regulatory T cell numbers are normal at day 12
• regulatory T cells are not able to suppress development of colitis in Rag1 null mice receiving na[?]ve T cells unlike wild-type regulatory T cells

growth/size/body

cellular




Genotype
MGI:5448151
cn4
Allelic
Composition
Foxo1tm1Flv/Foxo1tm1Flv
Gt(ROSA)26Sortm1(CAG-FOXO1,GFP)Moli/Gt(ROSA)26Sor+
Foxp3tm4(YFP/icre)Ayr/Foxp3+
Genetic
Background
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Foxp3tm4(YFP/icre)Ayr mutation (2 available); any Foxp3 mutation (58 available)
Gt(ROSA)26Sortm1(CAG-FOXO1,GFP)Moli mutation (1 available); any Gt(ROSA)26Sor mutation (993 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• the lethal inflammatory phenotype is completely rescued

immune system
N
• the lethal inflammatory phenotype is completely rescued




Genotype
MGI:3840970
cn5
Allelic
Composition
Foxo1tm1Flv/Foxo1tm1Flv
Rag1tm1Mom/Rag1tm1Mom
Tg(Cd4-cre)1Cwi/?
Tg(TcraTcrb)425Cbn/?
Genetic
Background
involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Rag1tm1Mom mutation (49 available); any Rag1 mutation (123 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• T cell numbers in the spleen and lymph nodes are reduced 80-90% compared to Rag-null OT-II controls

hematopoietic system
• T cell numbers in the spleen and lymph nodes are reduced 80-90% compared to Rag-null OT-II controls




Genotype
MGI:3840971
cn6
Allelic
Composition
Foxo1tm1Flv/Foxo1tm1Flv
Rag1tm1Mom/Rag1tm1Mom
Tg(CD2-Il7r)1Asin/?
Tg(Cd4-cre)1Cwi/?
Tg(TcraTcrb)425Cbn/?
Genetic
Background
involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Rag1tm1Mom mutation (49 available); any Rag1 mutation (123 available)
Tg(CD2-Il7r)1Asin mutation (0 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
Tg(TcraTcrb)425Cbn mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• the Il7r transgene rescues the low T cell numbers observed in mice with Foxo1-deficient OT-II T cells

hematopoietic system
• the Il7r transgene rescues the low T cell numbers observed in mice with Foxo1-deficient OT-II T cells




Genotype
MGI:3840969
cn7
Allelic
Composition
Foxo1tm1Flv/Foxo1tm1Flv
Tg(Cd4-cre)1Cwi/?
Genetic
Background
involves: 129S6/SvEvTac * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1tm1Flv mutation (2 available); any Foxo1 mutation (32 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• colitis results when mutant bone marrow is used to reconstitute irradiated Rag null mice
• in these bone marrow chimeras, only 7% of CD4+ T cells are regulatory T cells compared to 30% in control chimeras
• there is a slight increase in the number of single-positive Tcr-betahi CD4+ and CD8+ mature thymocytes
• despite increased proliferation upon activation, T cells have decreased homeostatic proliferation with only a10-20% recovery rate of wild-type in competitive bone marrow chimera assays
• a higher percentage of CD4+T cells activated in vitro produce IFN-gamma, IL-4, IL-10 or IL-17 compared to controls
• a higher percentage of CD8+ T cells activated in vitro produce IFN-gamma compared to controls
• an increased percentage of splenic T cells exhibit an activated CD44hiCD62Llo or CD69+ phenotype
• there is about a 3-fold increase in activated CD4 T cells and a 5- to 10- fold increase in activated CD8 T cells
• a higher percentage of these activated T cells can produce IL-17 or IFN-gamma upon restimulation compared to activated wild-type T cells
• mice have lymphadenopathy resulting from a rapidly proliferating CD4+ T cell population
• mice have lymphadenopathy resulting from a rapidly proliferating CD4+ T cell population
• mice 5-6 months of age have elevated levels of anti-nuclear antigens
• mice 5-6 months of age have elevated levels of anti-dsDNA antigens

digestive/alimentary system
• colitis results when mutant bone marrow is used to reconstitute irradiated Rag null mice
• in these bone marrow chimeras, only 7% of CD4+ T cells are regulatory T cells compared to 30% in control chimeras

hematopoietic system
• there is a slight increase in the number of single-positive Tcr-betahi CD4+ and CD8+ mature thymocytes
• despite increased proliferation upon activation, T cells have decreased homeostatic proliferation with only a10-20% recovery rate of wild-type in competitive bone marrow chimera assays
• a higher percentage of CD4+T cells activated in vitro produce IFN-gamma, IL-4, IL-10 or IL-17 compared to controls
• a higher percentage of CD8+ T cells activated in vitro produce IFN-gamma compared to controls
• an increased percentage of splenic T cells exhibit an activated CD44hiCD62Llo or CD69+ phenotype
• there is about a 3-fold increase in activated CD4 T cells and a 5- to 10- fold increase in activated CD8 T cells
• a higher percentage of these activated T cells can produce IL-17 or IFN-gamma upon restimulation compared to activated wild-type T cells
• mice have lymphadenopathy resulting from a rapidly proliferating CD4+ T cell population

cellular
• mice have lymphadenopathy resulting from a rapidly proliferating CD4+ T cell population





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory