Phenotypes associated with this allele

• Allele Symbol: Fto^tm1Urt
• Allele Name: targeted mutation 1, Ulrich Ruther
• Allele ID: MGI:3841156
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fto^tm1Urt/Fto^tm1Urt	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3841170
ht2	Fto^tm1Urt/Fto^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3841176
cx3	Fto^tm1Urt/Fto^tm1Urt Lep^ob/Lep^ob	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5688673

Genotype
MGI:3841170

hm1

• Allelic Composition: Fto^tm1Urt/Fto^tm1Urt
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:147471 )

• about 40% of mice die within the first 4 weeks of life

• lethality is reduced by postponed weaning or smaller litter size

growth/size/body

decreased lean body mass ( J:147471 )

• lean body mass is reduced by 26% and 19% in males and females, respectively

decreased body weight ( J:147471 )

• mice of both sexes weight 30-40% less than controls at 6 weeks of age

decreased body length ( J:147471 )

• nose-anus length of mutant mice is reduced compared to wild-type mice from 10.1 to 8.6 cm, and 10.3 to 9.4 cm, in males and females, respectively

decreased susceptibility to diet-induced obesity ( J:147471 )

• mice have less weight gain than controls after 12 weeks on a high-fat diet

postnatal growth retardation ( J:147471 )

• mice have growth retardation from day P2 onwards

behavior/neurological

polyphagia ( J:147471 )

• mice consume more food on a per kilogram bodyweight compared to age-matched controls

decreased locomotor activity ( J:147471 )

• mean activity over a 60 hour period is reduced more than 2.5-fold

homeostasis/metabolism

abnormal circulating hormone level ( J:147471 )

• adiponectin levels are slightly elevated

increased circulating adrenaline level ( J:147471 )

• mean adrenaline levels are elevated about 3-fold

decreased circulating leptin level ( J:147471 )

• leptin levels are reduced by about two-thirds

increased energy expenditure ( J:147471 )

• oxygen consumption and carbon dioxide production are significantly elevated both day and night despite decreased levels of activity

decreased susceptibility to diet-induced obesity ( J:147471 )

• mice have less weight gain than controls after 12 weeks on a high-fat diet

increased carbon dioxide production ( J:147471 )

• carbon dioxide production is significantly elevated both day and night

increased oxygen consumption ( J:147471 )

• oxygen consumption is significantly elevated both day and night

increased basal metabolism ( J:147471 )

• heat production is significantly elevated both day and night

adipose tissue

decreased total body fat amount ( J:147471 )

• fat mass is significantly reduced by 60% and 23% in males and females, respectively, compared to controls

decreased white fat cell size ( J:147471 )

• fat cell size is markedly reduced in these mice

decreased gonadal fat pad weight ( J:147471 )

• epigonadal fat pads are less than controls and are almost completely absent at 15 months of age

Genotype
MGI:3841176

ht2

• Allelic Composition: Fto^tm1Urt/Fto⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

growth/size/body

decreased susceptibility to diet-induced obesity ( J:147471 )

• mice have less weight gain and less body-fat percentage than controls after 12 weeks on a high-fat diet

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:147471 )

• mice have less weight gain and less body-fat percentage than controls after 12 weeks on a high-fat diet

Genotype
MGI:5688673

cx3

• Allelic Composition: Fto^tm1Urt/Fto^tm1Urt; Lep^ob/Lep^ob
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

adipose tissue

abnormal adipose tissue morphology ( J:219118 )

• marker analysis indicates transformation of white to brown adipose tissue

increased fat cell size ( J:219118 )

• increase in area size of epigonadal fat cells

increased gonadal fat pad weight ( J:219118 )

• increase in epigonadal fat tissue weight compared to wild-type mice but to a lesser extent than in single Lep^ob homozygotes

increased inguinal fat pad weight ( J:219118 )

increased interscapular fat pad weight ( J:219118 )

• increase in interscapular fat tissue weight compared to wild-type mice but to a lesser than in single Lep^ob homozygotes

increased percent body fat/body weight ( J:219118 )

• increase in fat mass at 16 weeks of age, but not at 8 weeks of age

behavior/neurological

decreased locomotor activity ( J:219118 )

• decrease in physical activity

endocrine/exocrine glands

enlarged pancreatic islets ( J:219118 )

• mice have bigger islet area per pancreas area than wild-type mice, but less islet area than in single Lep^ob homozygotes, and they never show giant islets

growth/size/body

increased body weight ( J:219118 )

• mice show an increase in weight gain during the first 8 weeks of life, however after 9 weeks, mice show a similar increase in body weight as wild-type mice

• 25% heavier than wild-type mice at 16 weeks of age

decreased body length ( J:219118 )

• mice exhibit reduced body length, similar to single Fto^tm1Urt homozygotes

increased liver weight ( J:219118 )

• increase in liver weight compared to wild-type mice but to a lesser extent than in single Lep^ob homozygotes

homeostasis/metabolism

insulin resistance ( J:219118 )

• mice are insulin resistant

• however, mice exhibit normal glucose tolerance and normal plasma glucose levels

liver/biliary system

increased liver weight ( J:219118 )

• increase in liver weight compared to wild-type mice but to a lesser extent than in single Lep^ob homozygotes

hepatic steatosis ( J:219118 )

• mice show decreased fat accumulation in the liver compared to single Lep^ob homozygotes but more than in wild-type mice