Phenotypes associated with this allele

• Allele Symbol: Tg(SFTPC-cre)11Yo
• Allele Name: transgene insertion 11, Yuichi Oike
• Allele ID: MGI:3841690
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rb1cc1^tm1.1Guan/Rb1cc1^tm1.1Guan Tg(SFTPC-cre)11Yo/0	involves: 129P2/OlaHsd * C57BL/6	MGI:6509968
cn2	Atg5^tm1Myok/Atg5^tm1Myok Tg(SFTPC-cre)11Yo/0	involves: 129S/SvEv * C57BL/6	MGI:6509969
cn3	Tg(CAG-ANGPT1*)5Yo/0 Tg(SFTPC-cre)11Yo/0	involves: C57BL/6 * CBA	MGI:5316896

Genotype
MGI:6509968

cn1

• Allelic Composition: Rb1cc1^tm1.1Guan/Rb1cc1^tm1.1Guan; Tg(SFTPC-cre)11Yo/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:300057 )

• mice are born at the expected Mendelian ratios but fail to survive beyond 24 hours after birth

respiratory system

abnormal alveolar lamellar body morphology ( J:300057 )

• EM of neonatal lungs revealed a reduction in the size and number of lamellar bodies

• however, newborns show no apparent defects in lung morphogenesis, type I pneumocyte differentiation, or maturation of surfactant protein-B (SPB)

decreased alveolar lamellar body number ( J:300057 )

small alveolar lamellar bodies ( J:300057 )

respiratory distress ( J:300057 )

• although mice initiate respiratory effort at birth, they exhibit signs of respiratory distress, such as cyanosis and gasping

respiratory failure ( J:300057 )

• H&E staining revealed that airspaces appear shrunken and septal walls are folded at 1 hour after birth

homeostasis/metabolism

cyanosis ( J:300057 )

• newborn mice exhibit cyanosis at 1 hour after birth

Genotype
MGI:6509969

cn2

• Allelic Composition: Atg5^tm1Myok/Atg5^tm1Myok; Tg(SFTPC-cre)11Yo/0
• Genetic Background: involves: 129S/SvEv * C57BL/6

mortality/aging

mortality/aging ( J:300057 )

N • mice do NOT exhibit neonatal lethality

Genotype
MGI:5316896

cn3

• Allelic Composition: Tg(CAG-ANGPT1*)5Yo/0; Tg(SFTPC-cre)11Yo/0
• Genetic Background: involves: C57BL/6 * CBA

mortality/aging

neonatal lethality, incomplete penetrance ( J:147388 )

• around 50% of pups die within a few hours of birth

growth/size/body

growth/size/body region phenotype ( J:147388 )

N • surviving animals show no obvious growth retardation compared to controls

respiratory system

abnormal lung vasculature morphology ( J:147388 )

• muscular vessels of the lungs are abnormally dilated compared to controls; diameter of pulmonary artery in vicinity of the terminal bronchiole is around 2-fold larger than in controls

• lungs display greater number of pulmonary vessel branches than controls

• most peripheral part of pulmonary small vessels is detectable, suggesting that proximal muscular pulmonary vessels are more dilated than in controls

increased bronchioalveolar stem cell number ( J:147388 )

• density of bronchioalveolar stem cells (BASCs) per whole lung cell number is 4-fold greater than in control lungs; however, total number of lung cells in one-third of control number so BASC density is actually similar between groups

abnormal pulmonary alveolus morphology ( J:147388 )

• alveolar density is about 50% of that of controls

respiratory distress ( J:147388 )

• observed in about half of newborn animals

cardiovascular system

abnormal lung vasculature morphology ( J:147388 )

• muscular vessels of the lungs are abnormally dilated compared to controls; diameter of pulmonary artery in vicinity of the terminal bronchiole is around 2-fold larger than in controls

• lungs display greater number of pulmonary vessel branches than controls

• most peripheral part of pulmonary small vessels is detectable, suggesting that proximal muscular pulmonary vessels are more dilated than in controls

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:147388 )

N • surviving animals show no difference in oxygen consumption rate from controls at rest

impaired exercise endurance ( J:147388 )

• exercise tolerance periods are significantly shorter than single transgenic controls

cyanosis ( J:147388 )

• around 50% of pups exhibit severe cyanosis and expire

abnormal oxygen consumption ( J:147388 )

• mice show defective physiological response of oxygen consumption rate to exercise compared to controls

behavior/neurological

impaired exercise endurance ( J:147388 )

• exercise tolerance periods are significantly shorter than single transgenic controls