Phenotypes associated with this allele

• Allele Symbol: Tg(CAG-ANGPT1*)5Yo
• Allele Name: transgene insertion 5, Yuichi Oike
• Allele ID: MGI:3841702
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(cre/ERT2)Tyj/Gt(ROSA)26Sor^+ Tg(CAG-ANGPT1*)5Yo/0	involves: 129S4/SvJae * C57BL/6NCrlj * CBA/JNCrlj	MGI:5585468
cn2	Tg(CAG-ANGPT1*)5Yo/0 Tg(SFTPC-cre)11Yo/0	involves: C57BL/6 * CBA	MGI:5316896

Genotype
MGI:5585468

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}/Gt(ROSA)26Sor⁺; Tg(CAG-ANGPT1*)5Yo/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6NCrlj * CBA/JNCrlj

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal retina blood vessel morphology ( J:213456 )

• tamoxifen-treated mice exhibit increased radial length (1.5-fold), vascular density (1.6-fold), vessel diameter (1.9-fold), endothelial cell proliferative activity (2.0-fold) and sprouting activity (1.8-fold) compared with control mice

decreased vascular permeability ( J:213456 )

• 2.4-fold decreased vessel leakage following oxygen-induced retinopathy in tamoxifen-treated mice

vision/eye

abnormal retina blood vessel morphology ( J:213456 )

• tamoxifen-treated mice exhibit increased radial length (1.5-fold), vascular density (1.6-fold), vessel diameter (1.9-fold), endothelial cell proliferative activity (2.0-fold) and sprouting activity (1.8-fold) compared with control mice

decreased susceptilbility to retina ischemic injury ( J:213456 )

• following oxygen-induced retinopathy, mice exhibit decreased avascular area, neovascular tuft area and increased pericyte coverage in the superficial layer with more densely networked vessels in the central and middle regions of the deep layer, 2.4-fold decreased vessel leakage and 8.6-fold decrease in multifocal hemorrhages compared with control mice

nervous system

increased astrocyte number ( J:213456 )

• 1.3-fold increase in GFAP+ cell densities in tamoxifen-treated mice

• however, the number of Pax2+ astrocytes is normal

homeostasis/metabolism

decreased susceptilbility to retina ischemic injury ( J:213456 )

• following oxygen-induced retinopathy, mice exhibit decreased avascular area, neovascular tuft area and increased pericyte coverage in the superficial layer with more densely networked vessels in the central and middle regions of the deep layer, 2.4-fold decreased vessel leakage and 8.6-fold decrease in multifocal hemorrhages compared with control mice

Genotype
MGI:5316896

cn2

• Allelic Composition: Tg(CAG-ANGPT1*)5Yo/0; Tg(SFTPC-cre)11Yo/0
• Genetic Background: involves: C57BL/6 * CBA

mortality/aging

neonatal lethality, incomplete penetrance ( J:147388 )

• around 50% of pups die within a few hours of birth

growth/size/body

growth/size/body region phenotype ( J:147388 )

N • surviving animals show no obvious growth retardation compared to controls

respiratory system

abnormal lung vasculature morphology ( J:147388 )

• muscular vessels of the lungs are abnormally dilated compared to controls; diameter of pulmonary artery in vicinity of the terminal bronchiole is around 2-fold larger than in controls

• lungs display greater number of pulmonary vessel branches than controls

• most peripheral part of pulmonary small vessels is detectable, suggesting that proximal muscular pulmonary vessels are more dilated than in controls

increased bronchioalveolar stem cell number ( J:147388 )

• density of bronchioalveolar stem cells (BASCs) per whole lung cell number is 4-fold greater than in control lungs; however, total number of lung cells in one-third of control number so BASC density is actually similar between groups

abnormal pulmonary alveolus morphology ( J:147388 )

• alveolar density is about 50% of that of controls

respiratory distress ( J:147388 )

• observed in about half of newborn animals

cardiovascular system

abnormal lung vasculature morphology ( J:147388 )

• muscular vessels of the lungs are abnormally dilated compared to controls; diameter of pulmonary artery in vicinity of the terminal bronchiole is around 2-fold larger than in controls

• lungs display greater number of pulmonary vessel branches than controls

• most peripheral part of pulmonary small vessels is detectable, suggesting that proximal muscular pulmonary vessels are more dilated than in controls

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:147388 )

N • surviving animals show no difference in oxygen consumption rate from controls at rest

impaired exercise endurance ( J:147388 )

• exercise tolerance periods are significantly shorter than single transgenic controls

cyanosis ( J:147388 )

• around 50% of pups exhibit severe cyanosis and expire

abnormal oxygen consumption ( J:147388 )

• mice show defective physiological response of oxygen consumption rate to exercise compared to controls

behavior/neurological

impaired exercise endurance ( J:147388 )

• exercise tolerance periods are significantly shorter than single transgenic controls