All Phenotypes Kcng4<tm1(KOMP)Vlcg> MGI Mouse

decreased sperm progressive motility ( J:302033 )

• progressive motility is only 5%, i.e. significantly less than that in wild-type males (73 +/- 7%)

asthenozoospermia ( J:302033 )

• only a few spermatozoa are found to be motile in semen

abnormal spermatogenesis ( J:302033 )

• differentiation of round spermatids into elongated spermatids is disturbed, suggesting abnormalities in the post-meiotic stages of spermatogenesis

• no differences are observed in the number or morphology of spermatogonia, spermatocytes, round spermatids and Sertoli cells

necrospermia ( J:302033 )

• eosin-nigrosin staining revealed that the fraction of dead spermatozoa detected in semen is significantly higher (61 +/- 5%) than that in wild-type males (28 +/- 2%)

oligozoospermia ( J:302033 )

• at 12-16 weeks of age, the number of cauda epididymal spermatozoa is significantly lower than that isolated from wild-type males

• H&E staining of caudae epididymides revealed only a few spermatozoa in the lumen of epididymal ductules

teratozoospermia ( J:302033 )

• analysis of the few spermatozoa produced revealed a significant reduction in the number of protoplasmic distal droplets, suggesting that epididymal maturation is affected

abnormal sperm midpiece morphology ( J:302033 )

• the % of midpiece abnormalities is significantly higher (25.0 +/- 0.9%) than that observed in wild-type semen (9.3 +/- 1.0%)

short sperm flagellum ( J:302033 )

• the sperm tail length is significantly reduced

abnormal sperm head morphology ( J:302033 )

• nearly all spermatozoa exhibit an abnormally shaped sperm head

small sperm head ( J:302033 )

• the sperm head area is significantly reduced

abnormal spermatid morphology ( J:302033 )

• elongated spermatids appear morphologically abnormal and are severely reduced in number

abnormal spermiogenesis ( J:302033 )

• spermiogenesis proceeds abnormally; elongated spermatids appear morphologically abnormal and are severely reduced in number in a large fraction of the seminiferous tubules

male infertility ( J:302033 )

• at 10-14 weeks of age, males are sterile; offspring from homozygous females can only be obtained after mating with wild-type or heterozygous males

• however, mating behavior, ejaculation, and vaginal plug formation are normal