Phenotypes associated with this allele

• Allele Symbol: Nkx3-2^tm1(cre)Wez
• Allele Name: targeted mutation 1, Warren E Zimmer
• Allele ID: MGI:3848005
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nkx3-2^tm1(cre)Wez/Nkx3-2^tm1(cre)Wez	involves: 129S7/SvEvBrd * C57BL/6	MGI:3848515
cn2	Gt(ROSA)26Sor^tm1(Gli2)Jmao/Gt(ROSA)26Sor^+ Smo^tm2Amc/Smo^tm2Amc Nkx3-2^tm1(cre)Wez/Nkx3-2^+	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ	MGI:5518632
cn3	Smo^tm2Amc/Smo^tm2Amc Nkx3-2^tm1(cre)Wez/Nkx3-2^+	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ	MGI:5518634
cn4	Gli3^tm1Alj/Gli3^tm1Alj Nkx3-2^tm1(cre)Wez/Nkx3-2^+	involves: 129S6/SvEvTac * 129S7/SvEvBrd	MGI:5518635
cn5	Gli3^tm1Alj/Gli3^tm1Alj Nkx3-2^tm1(cre)Wez/Nkx3-2^+ Smo^tm2Amc/Smo^tm2Amc	involves: 129S6/SvEvTac * 129S7/SvEvBrd * 129X1/SvJ	MGI:5518637
cn6	Gt(ROSA)26Sor^tm1(Gli2)Jmao/Gt(ROSA)26Sor^+ Nkx3-2^tm1(cre)Wez/Nkx3-2^+	involves: 129S7/SvEvBrd	MGI:5518631
cn7	Gt(ROSA)26Sor^tm1(Smo/EYFP)Amc/Gt(ROSA)26Sor^+ Nkx3-2^tm1(cre)Wez/Nkx3-2^+	involves: 129S7/SvEvBrd * 129X1/SvJ	MGI:5518633

Genotype
MGI:3848515

hm1

• Allelic Composition: Nkx3-2^tm1(cre)Wez/Nkx3-2^tm1(cre)Wez
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

craniofacial

absent basioccipital bone ( J:148724 )

• loss of this bone of the cranial vault is observed at E18.5

absent basisphenoid bone ( J:148724 )

• observed at E18.5

skeleton

abnormal skeleton morphology ( J:148724 )

• skeletons are defective; skeletal anomalies are similar to those reported for other Nkx3-2 null homozygotes

absent basioccipital bone ( J:148724 )

• loss of this bone of the cranial vault is observed at E18.5

absent basisphenoid bone ( J:148724 )

• observed at E18.5

abnormal vertebral column morphology ( J:148724 )

• absence of midline structures in vertebral column is observed in E18.5 embryos

Genotype
MGI:5518632

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Gli2)Jmao}/Gt(ROSA)26Sor⁺; Smo^tm2Amc/Smo^tm2Amc; Nkx3-2^tm1(cre)Wez/Nkx3-2⁺
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ

Gli2 expression, but not Gli3 de-repression, rescues intestinal phenotypes in Smo^tm2Amc/Smo^tm2Amc Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ embryos

digestive/alimentary system

digestive/alimentary phenotype ( J:199664 )

N • intestinal development is rescued

Genotype
MGI:5518634

cn3

• Allelic Composition: Smo^tm2Amc/Smo^tm2Amc; Nkx3-2^tm1(cre)Wez/Nkx3-2⁺
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ

Intestinal mesenchymal proliferation and expansion are affected in Smo^tm2Amc/Smo^tm2Amc Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ embryos

mortality/aging

neonatal lethality, complete penetrance ( J:199664 )

• mice die immediately after birth

digestive/alimentary system

abnormal intestinal goblet cell morphology ( J:199664 )

• lack of Goblet and enteroendocrine cell lineage differentiation at E18.5

abnormal intestine development ( J:199664 )

• at E18.5, mice exhibit fewer mesenchyme cells between thin layers of mesentery and intestinal epithelium compared with control mice

abnormal intestinal enteroendocrine cell morphology ( J:199664 )

• lack of Goblet and enteroendocrine cell lineage differentiation at E18.5

abnormal intestinal smooth muscle morphology ( J:199664 )

• decreased SMA+ or Desmin+ intestinal smooth muscle cells

abnormal intestine physiology ( J:199664 )

• absent intestinal mesenchymal proliferation

abnormal enterocyte proliferation ( J:199664 )

• intestinal epithelium do not proliferate at E18.5

muscle

abnormal intestinal smooth muscle morphology ( J:199664 )

• decreased SMA+ or Desmin+ intestinal smooth muscle cells

cellular

abnormal intestinal goblet cell morphology ( J:199664 )

• lack of Goblet and enteroendocrine cell lineage differentiation at E18.5

abnormal enterocyte proliferation ( J:199664 )

• intestinal epithelium do not proliferate at E18.5

nervous system

abnormal enteric neuron morphology ( J:199664 )

• decreased population

Genotype
MGI:5518635

cn4

• Allelic Composition: Gli3^tm1Alj/Gli3^tm1Alj; Nkx3-2^tm1(cre)Wez/Nkx3-2⁺
• Genetic Background: involves: 129S6/SvEvTac * 129S7/SvEvBrd

Gli2 expression, but not Gli3 de-repression, rescues intestinal phenotypes in Smo^tm2Amc/Smo^tm2Amc Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ embryos

mortality/aging

mortality/aging ( J:199664 )

N • mice are born at the expected Mendelian ratios

digestive/alimentary system

digestive/alimentary phenotype ( J:199664 )

N • mice exhibit normal intestinal development

Genotype
MGI:5518637

cn5

• Allelic Composition: Gli3^tm1Alj/Gli3^tm1Alj; Nkx3-2^tm1(cre)Wez/Nkx3-2⁺; Smo^tm2Amc/Smo^tm2Amc
• Genetic Background: involves: 129S6/SvEvTac * 129S7/SvEvBrd * 129X1/SvJ

Gli2 expression, but not Gli3 de-repression, rescues intestinal phenotypes in Smo^tm2Amc/Smo^tm2Amc Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ embryos

digestive/alimentary system

abnormal digestive system morphology ( J:199664 )

• mice exhibit the same defects as in Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ Smo^m2Amc/Smo^m2Amc mice

Genotype
MGI:5518631

cn6

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Gli2)Jmao}/Gt(ROSA)26Sor⁺; Nkx3-2^tm1(cre)Wez/Nkx3-2⁺
• Genetic Background: involves: 129S7/SvEvBrd

Gli2 expression, but not Gli3 de-repression, rescues intestinal phenotypes in Smo^tm2Amc/Smo^tm2Amc Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ embryos

digestive/alimentary system

digestive/alimentary phenotype ( J:199664 )

N • mice exhibit normal intestinal development

Genotype
MGI:5518633

cn7

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc}/Gt(ROSA)26Sor⁺; Nkx3-2^tm1(cre)Wez/Nkx3-2⁺
• Genetic Background: involves: 129S7/SvEvBrd * 129X1/SvJ

digestive/alimentary system

abnormal intestine development ( J:199664 )

• intestinal mesenchymal compartment is expanded