Phenotypes associated with this allele

• Allele Symbol: Plxnd1^tm1.1Tmj
• Allele Name: targeted mutation 1.1, Thomas M Jessell
• Allele ID: MGI:3848812
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Plxnd1^tm1.1Tmj/Plxnd1^tm1.1Tmj Tg(CAG-cre/Esr1*)5Amc/0	involves: 129S/SvEv * 129S4/SvJaeSor * C57BL/6 * CBA	MGI:3848824
cn2	Plxnd1^tm1.1Tmj/Plxnd1^tm1.1Tmj H2az2^Tg(Wnt1-cre)11Rth/H2az2^+	involves: 129S/SvEv * 129S4/SvJaeSor * C57BL/6 * CBA/J	MGI:3848822
cn3	Plxnd1^tm1.1Tmj/Plxnd1^tm1.1Tmj Tg(Tek-cre)1Ywa/0	involves: 129S/SvEv * 129S4/SvJaeSor * C57BL/6 * SJL	MGI:3848821
cn4	Plxnd1^tm1Ddg/Plxnd1^tm1.1Tmj H2az2^Tg(Wnt1-cre)11Rth/H2az2^+	involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA/J	MGI:3848832
cn5	Plxnd1^tm1Ddg/Plxnd1^tm1.1Tmj Tg(CAG-cre/Esr1*)1Egwa/0	involves: 129S/SvEv * C57BL/6 * DBA/2	MGI:5052373
cn6	Plxnd1^tm1Ddg/Plxnd1^tm1.1Tmj H2az2^Tg(Wnt1-cre)11Rth/H2az2^+	involves: 129S/SvEv * C57BL/6J * CBA/J	MGI:5550533
cn7	Plxnd1^tm1.1Tmj/Plxnd1^tm1.1Tmj Tg(Nes-cre)1Atp/0	involves: 129S/SvEv * FVB/N	MGI:5007643

Genotype
MGI:3848824

cn1

• Allelic Composition: Plxnd1^tm1.1Tmj/Plxnd1^tm1.1Tmj; Tg(CAG-cre/Esr1*)5Amc/0
• Genetic Background: involves: 129S/SvEv * 129S4/SvJaeSor * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal retina vasculature morphology ( J:143763 )

• tamoxifen-treated retinas exhibit reduced angiogenesis and hemorrhages compared to in wild-type mice

decreased angiogenesis ( J:143763 )

• tamoxifen-treated retinas exhibit reduced angiogenesis compared to in wild-type mice

abnormal vascular endothelial cell migration ( J:143763 )

• vascular invasion of tamoxifen-treated endothelial cells into matrigel plugs treated with endothelial cell growth factor is deficient compared to that of wild-type cells

hemorrhage ( J:143763 )

• tamoxifen-treated retinas exhibit hemorrhages unlike wild-type retinas

vision/eye

abnormal retina vasculature morphology ( J:143763 )

• tamoxifen-treated retinas exhibit reduced angiogenesis and hemorrhages compared to in wild-type mice

cellular

abnormal vascular endothelial cell migration ( J:143763 )

• vascular invasion of tamoxifen-treated endothelial cells into matrigel plugs treated with endothelial cell growth factor is deficient compared to that of wild-type cells

Genotype
MGI:3848822

cn2

• Allelic Composition: Plxnd1^tm1.1Tmj/Plxnd1^tm1.1Tmj; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺
• Genetic Background: involves: 129S/SvEv * 129S4/SvJaeSor * C57BL/6 * CBA/J

mortality/aging

mortality/aging ( J:143763 )

N • normal numbers of mice are born

cardiovascular system

cardiovascular system phenotype ( J:143763 )

N • mice exhibit normal cardiac development with normal outflow tract septation

Genotype
MGI:3848821

cn3

• Allelic Composition: Plxnd1^tm1.1Tmj/Plxnd1^tm1.1Tmj; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129S/SvEv * 129S4/SvJaeSor * C57BL/6 * SJL

mortality/aging

postnatal lethality, complete penetrance ( J:143763 )

• all but 1 mouse died by P2

neonatal lethality, incomplete penetrance ( J:143763 )

• many mice die by P1

cardiovascular system

abnormal blood vessel morphology ( J:143763 )

• mice exhibit abnormal microvasculature within bones

• however, large vessels attached to the long bones appear intact

abnormal aorta morphology ( J:143763 )

• the root of the aorta is located more distally from the ascending portion of the truncus arteriosis or from the ascending aorta than in wild-type mice

right aortic arch ( J:143763 )

• in most mice

abnormal intersomitic vessel morphology ( J:143763 )

• at E11.5, mice exhibit poor remodeling of the vascular plexus of the immature intersomitic vessels unlike in wild-type mice

abnormal myocardium layer morphology ( J:143763 )

• mice exhibit abnormally condensed discontinuous atrial myocardial architecture unlike in wild-type mice

• the atrial myocardium is separated from the epicardium unlike in wild-type mice

• the ventricular myocardial wall is abnormal and unusual epicardial blood islands unlike in wild-type mice

abnormal truncus arteriosus septation ( J:143763 )

• the outflow tract is malrotated compared to in wild-type mice

persistent truncus arteriosus ( J:143763 )

• in most mice

abnormal coronary vessel morphology ( J:143763 )

• at E12.5, mice exhibit coronary vessel defects

• originate more distally from the ascending portion of the truncus arteriosus, or from the ascending aorta (when partial or compete septation was present) in all mutant hearts examined (N=5)

abnormal heart atrium morphology ( J:143763 )

• the atria appear raspberry-like

• mice exhibit abnormally condensed discontinuous atrial myocardial architecture unlike in wild-type mice

dilated heart atrium ( J:143763 )

ventricular septal defect ( J:143763 )

detached epicardium ( J:143763 )

• the atrial myocardium is separated from the epicardium unlike in wild-type mice

• the ventricular myocardial wall is abnormal and unusual epicardial blood islands are present unlike in wild-type mice

hemorrhage ( J:143763 )

• mice exhibit subcutaneous hemorrhage, especially in the head, unlike in wild-type mice

• small hemorrhages are observed in the ventricular walls unlike in wild-type mice

intracranial hemorrhage ( J:143763 )

skeleton

rib fusion ( J:143763 )

• at E15.5

abnormal vertebral body morphology ( J:143763 )

• poorly formed and frequently fused or split in the lumbar and thoracic regions

fusion of vertebral bodies ( J:143763 )

• frequently fused in the lumbar and thoracic regions

• in the one mouse that survives into adulthood, vertebral bodies are fused along the entire anterior-posterior axis

nervous system

intracranial hemorrhage ( J:143763 )

homeostasis/metabolism

cyanosis ( J:143763 )

• at birth

growth/size/body

decreased body height ( J:143763 )

limbs/digits/tail

short tail ( J:143763 )

muscle

abnormal myocardium layer morphology ( J:143763 )

• mice exhibit abnormally condensed discontinuous atrial myocardial architecture unlike in wild-type mice

• the atrial myocardium is separated from the epicardium unlike in wild-type mice

• the ventricular myocardial wall is abnormal and unusual epicardial blood islands unlike in wild-type mice

Genotype
MGI:3848832

cn4

• Allelic Composition: Plxnd1^tm1Ddg/Plxnd1^tm1.1Tmj; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA/J

nervous system

abnormal motor neuron morphology ( J:149553 )

• mice exhibit a 3-fold increase in the number of proprioceptive synaptic contacts with identified cutaneous maximus motor neurons compared to in wild-type mice

abnormal motor neuron innervation pattern ( J:149553 )

• 43% of cutaneous motor neurons receive monosynaptic input after stimulation of cutaneous maximus afferents unlike wild-type motor neurons

• however, monosynaptic specificity from the tricep is normal

behavior/neurological

behavior/neurological phenotype ( J:149553 )

N • mice exhibit no behavior deficits

Genotype
MGI:5052373

cn5

• Allelic Composition: Plxnd1^tm1Ddg/Plxnd1^tm1.1Tmj; Tg(CAG-cre/Esr1*)1Egwa/0
• Genetic Background: involves: 129S/SvEv * C57BL/6 * DBA/2

Increase in extraretinal vascular outgrowth in ischemic Plxnd1^tm1.1Tmj/Plxnd1^tm1Ddg Tg(CAG-cre/Esr1*)1Egwa/0 retinas

vision/eye

abnormal induced retina neovascularization ( J:173943 )

• tamoxifen-treated mice exhibit increased extraretinal vascular outgrowth in ischemic retinas compared with control mice

cardiovascular system

abnormal induced retina neovascularization ( J:173943 )

• tamoxifen-treated mice exhibit increased extraretinal vascular outgrowth in ischemic retinas compared with control mice

Genotype
MGI:5550533

cn6

• Allelic Composition: Plxnd1^tm1Ddg/Plxnd1^tm1.1Tmj; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6J * CBA/J

nervous system

nervous system phenotype ( J:205528 )

N • the patterns of hamstring knee flexor muscle and gluteus homonymous monosynaptic sensory-motor connections are similar to controls

abnormal excitatory postsynaptic potential ( J:205528 )

• a large amplitude of monosynaptic EPSP is seen in 45% of gluteus motor neurons unlike in controls

Genotype
MGI:5007643

cn7

• Allelic Composition: Plxnd1^tm1.1Tmj/Plxnd1^tm1.1Tmj; Tg(Nes-cre)1Atp/0
• Genetic Background: involves: 129S/SvEv * FVB/N

vision/eye

vision/eye phenotype ( J:172268 )

N • mice exhibit normal retinal stratification