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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Mecomtm1Miku
targeted mutation 1, Mineo Kurokawa
MGI:3849413
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Mecomtm1Miku/Mecomtm1Miku
Prdm16tm1.1Brsp/Prdm16tm1.1Brsp
Myf5tm1(cre)Mrc/Myf5+ 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6NCrlj * CBA/JCrlj MGI:6107171
cn2
 		Mecomtm1Miku/Mecomtm1Miku
Tg(Tek-cre)12Flv/0 		involves: C3H * C57BL/6 * CBA MGI:3849426
cn3
 		Mecomtm1Miku/Mecom+
Tg(Tek-cre)12Flv/0 		involves: C3H * C57BL/6 * CBA MGI:3849427
cn4
 		Mecomtm1Miku/Mecomtm1Miku
Tg(Mx1-cre)1Cgn/0 		involves: C57BL/6 * CBA MGI:3849428


Genotype
MGI:6107171
cn1
Allelic
Composition		 Mecomtm1Miku/Mecomtm1Miku
Prdm16tm1.1Brsp/Prdm16tm1.1Brsp
Myf5tm1(cre)Mrc/Myf5+
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6NCrlj * CBA/JCrlj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mecomtm1Miku mutation (0 available); any Mecom mutation (81 available)
Myf5tm1(cre)Mrc mutation (1 available); any Myf5 mutation (17 available)
Prdm16tm1.1Brsp mutation (1 available); any Prdm16 mutation (72 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
adipose tissue
increased brown fat cell lipid droplet size ( J:213153 )
• in the interscapular brown adipose tissue of mice older than 6 months
abnormal interscapular fat pad morphology ( J:213153 )
• pale in 3 month old mice




Genotype
MGI:3849426
cn2
Allelic
Composition		 Mecomtm1Miku/Mecomtm1Miku
Tg(Tek-cre)12Flv/0
Genetic
Background		 involves: C3H * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mecomtm1Miku mutation (0 available); any Mecom mutation (81 available)
Tg(Tek-cre)12Flv mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
lethality throughout fetal growth and development, incomplete penetrance ( J:149794 )
• lethality occurs between E13.5 and E16.5 for most embryos
• a few mice reach adulthood due to the presence of bone marrow cells that escape cre mediated excision

cardiovascular system
hemorrhage ( J:149794 )
• many E13.5 embryos exhibit hemorrhaging

hematopoietic system
decreased hematopoietic stem cell number ( J:149794 )
• the population of lin- Kit+CD34+ found among E14.5 fetal liver cells is severely reduced
• the number of myeloid or mixed CFUs isolated from fetal liver is also diminished

homeostasis/metabolism
skin edema ( J:149794 )
• many E13.5 embryos exhibit subcutaneous edema

integument
skin edema ( J:149794 )
• many E13.5 embryos exhibit subcutaneous edema




Genotype
MGI:3849427
cn3
Allelic
Composition		 Mecomtm1Miku/Mecom+
Tg(Tek-cre)12Flv/0
Genetic
Background		 involves: C3H * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mecomtm1Miku mutation (0 available); any Mecom mutation (81 available)
Tg(Tek-cre)12Flv mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased hematopoietic stem cell number ( J:149794 )
• the population of lin- Kit+CD34+ found among E14.5 fetal liver cells is somewhat reduced




Genotype
MGI:3849428
cn4
Allelic
Composition		 Mecomtm1Miku/Mecomtm1Miku
Tg(Mx1-cre)1Cgn/0
Genetic
Background		 involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mecomtm1Miku mutation (0 available); any Mecom mutation (81 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal platelet morphology ( J:149794 )
• platelets have delayed recovery of numbers after administration of Fluorouracil
thrombocytopenia ( J:149794 )
• platelet cell number is moderately decreased compared to controls 4 weeks after cre induction
abnormal hematopoietic stem cell morphology ( J:149794 )
• bone marrow HSC have reduced proliferation relative to controls when cultured in vitro
• both accelerated apoptosis and impaired cell cycling occur in these cells
• increased rates of apoptosis are observed in vivo in the fetal liver after cre induction
decreased hematopoietic stem cell number ( J:149794 )
• HSC and CFU numbers in the bone marrow significantly decrease after cre induction
• 12 weeks after induction, HSC numbers have recovered but consist entirely of HSC that have escaped cre-mediated excision of the gene locus




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
08/02/2024
MGI 6.24 		The Jackson Laboratory