Phenotypes associated with this allele

• Allele Symbol: Mecom^tm1Miku
• Allele Name: targeted mutation 1, Mineo Kurokawa
• Allele ID: MGI:3849413
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Mecom^tm1Miku/Mecom^tm1Miku Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Myf5^tm1(cre)Mrc/Myf5^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NCrlj * CBA/JCrlj	MGI:6107171
cn2	Mecom^tm1Miku/Mecom^tm1Miku Tg(Tek-cre)12Flv/0	involves: C3H * C57BL/6 * CBA	MGI:3849426
cn3	Mecom^tm1Miku/Mecom^+ Tg(Tek-cre)12Flv/0	involves: C3H * C57BL/6 * CBA	MGI:3849427
cn4	Mecom^tm1Miku/Mecom^tm1Miku Tg(Mx1-cre)1Cgn/0	involves: C57BL/6 * CBA	MGI:3849428

Genotype
MGI:6107171

cn1

• Allelic Composition: Mecom^tm1Miku/Mecom^tm1Miku; Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp; Myf5^tm1(cre)Mrc/Myf5⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6NCrlj * CBA/JCrlj

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

increased brown fat cell lipid droplet size ( J:213153 )

• in the interscapular brown adipose tissue of mice older than 6 months

abnormal interscapular fat pad morphology ( J:213153 )

• pale in 3 month old mice

Genotype
MGI:3849426

cn2

• Allelic Composition: Mecom^tm1Miku/Mecom^tm1Miku; Tg(Tek-cre)12Flv/0
• Genetic Background: involves: C3H * C57BL/6 * CBA

mortality/aging

lethality throughout fetal growth and development, incomplete penetrance ( J:149794 )

• lethality occurs between E13.5 and E16.5 for most embryos

• a few mice reach adulthood due to the presence of bone marrow cells that escape cre mediated excision

cardiovascular system

hemorrhage ( J:149794 )

• many E13.5 embryos exhibit hemorrhaging

hematopoietic system

decreased hematopoietic stem cell number ( J:149794 )

• the population of lin^- Kit⁺CD34⁺ found among E14.5 fetal liver cells is severely reduced

• the number of myeloid or mixed CFUs isolated from fetal liver is also diminished

homeostasis/metabolism

skin edema ( J:149794 )

• many E13.5 embryos exhibit subcutaneous edema

integument

skin edema ( J:149794 )

• many E13.5 embryos exhibit subcutaneous edema

Genotype
MGI:3849427

cn3

• Allelic Composition: Mecom^tm1Miku/Mecom⁺; Tg(Tek-cre)12Flv/0
• Genetic Background: involves: C3H * C57BL/6 * CBA

hematopoietic system

decreased hematopoietic stem cell number ( J:149794 )

• the population of lin^- Kit⁺CD34⁺ found among E14.5 fetal liver cells is somewhat reduced

Genotype
MGI:3849428

cn4

• Allelic Composition: Mecom^tm1Miku/Mecom^tm1Miku; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * CBA

hematopoietic system

abnormal platelet morphology ( J:149794 )

• platelets have delayed recovery of numbers after administration of Fluorouracil

thrombocytopenia ( J:149794 )

• platelet cell number is moderately decreased compared to controls 4 weeks after cre induction

abnormal hematopoietic stem cell morphology ( J:149794 )

• bone marrow HSC have reduced proliferation relative to controls when cultured in vitro

• both accelerated apoptosis and impaired cell cycling occur in these cells

• increased rates of apoptosis are observed in vivo in the fetal liver after cre induction

decreased hematopoietic stem cell number ( J:149794 )

• HSC and CFU numbers in the bone marrow significantly decrease after cre induction

• 12 weeks after induction, HSC numbers have recovered but consist entirely of HSC that have escaped cre-mediated excision of the gene locus