Phenotypes associated with this allele

• Allele Symbol: Mecom^tm1.1Miku
• Allele Name: targeted mutation 1.1, Mineo Kurokawa
• Allele ID: MGI:3849414
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mecom^tm1.1Miku/Mecom^tm1.1Miku	B6.Cg-Mecom^tm1.1Miku	MGI:3849424
ht2	Mecom^tm1.1Miku/Mecom^+	B6.Cg-Mecom^tm1.1Miku	MGI:3849425
ht3	Mecom^tm1.1Miku/Mecom^+	involves: C57BL/6NCrlj * CBA/JNCrlj	MGI:5306624

Genotype
MGI:3849424

hm1

• Allelic Composition: Mecom^tm1.1Miku/Mecom^tm1.1Miku
• Genetic Background: B6.Cg-Mecom^tm1.1Miku

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:149794 )

• lethality occurs between E13.5 and E16.5

embryo

abnormal vitelline vasculature morphology ( J:149794 )

• yolk sacs show defective large vessel development

pale yolk sac ( J:149794 )

• yolk sacs of E13.5 embyros show severe anemia

hematopoietic system

abnormal hematopoietic stem cell morphology ( J:149794 )

• fetal liver cells fail to rescue lethally irradiated mice as wild-type fetal liver cells do

• no mutant cells are detected in competitive reconstitution assays

decreased hematopoietic stem cell number ( J:149794 )

• less HSC are cultured from E9.5 para-aortic splanchnopleural region explants compared to controls

abnormal fetal liver hematopoietic progenitor cell morphology ( J:149794 )

• the population of lin^- Kit⁺CD34⁺ found among E14.5 fetal liver cells is virtually absent

• the number of CFUs isolated from fetal liver is also severely diminished

cardiovascular system

abnormal vitelline vasculature morphology ( J:149794 )

• yolk sacs show defective large vessel development

hemorrhage ( J:149794 )

• many E13.5 embryos exhibit hemorrhaging

homeostasis/metabolism

skin edema ( J:149794 )

• many E13.5 embryos exhibit subcutaneous edema

integument

skin edema ( J:149794 )

• many E13.5 embryos exhibit subcutaneous edema

Genotype
MGI:3849425

ht2

• Allelic Composition: Mecom^tm1.1Miku/Mecom⁺
• Genetic Background: B6.Cg-Mecom^tm1.1Miku

hematopoietic system

abnormal platelet morphology ( J:149794 )

• platelets have delayed recovery of numbers after administration of Fluorouracil

abnormal hematopoietic stem cell morphology ( J:149794 )

• less mutant HSC than wild-type HSC reconstitute the bone marrow of lethally irradiated mice in competitive reconstitution assays

decreased hematopoietic stem cell number ( J:149794 )

• the population of lin^- Kit⁺CD34⁺ found among E14.5 fetal liver cells 2-3 fold compared to controls

• this population is also reduced by about half in the bone marrow of adult mice

Genotype
MGI:5306624

ht3

• Allelic Composition: Mecom^tm1.1Miku/Mecom⁺
• Genetic Background: involves: C57BL/6NCrlj * CBA/JNCrlj

hematopoietic system

decreased hematopoietic stem cell number ( J:178616 )

• mice exhibit an almost complete loss of long-term hematopoietic stem cells in a cell-autonomous manner compared with wild-type mice

abnormal hematopoietic stem cell physiology ( J:178616 )

• short-term and long-term hematopoietic stem cells self-renewal capacity is abrogated, as measured by reduced colony-forming units (granulocyte-macrophage and granulocyte-erythrocyte-monocyte-megakaryocyte progenitors) and erythroid burst-forming units, compared with wild-type cells