All Phenotypes Nlrp3<tm1Hhf> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nlrp3^tm1Flv/Nlrp3^tm1Hhf Lyz2^tm1(cre)Ifo/Lyz2⁺	involves: 129 * C57BL/6	MGI:3850059
cn2	Il1r1^tm1Roml/Il1r1^tm1Roml Nlrp3^tm1Hhf/Nlrp3⁺ Lyz2^tm1(cre)Ifo/Lyz2⁺	involves: 129 * C57BL/6	MGI:3850053
cn3	Nlrp3^tm1Hhf/Nlrp3⁺ Pycard^tm1Flv/Pycard^tm1Flv Lyz2^tm1(cre)Ifo/Lyz2⁺	involves: 129 * C57BL/6	MGI:3850058
cn4	Nlrp3^tm1Hhf/Nlrp3⁺ Rag1^tm1Mom/Rag1^tm1Mom Lyz2^tm1(cre)Ifo/Lyz2⁺	involves: 129/Sv * 129P2/OlaHsd * C57BL/6	MGI:3850056
cn5	Nlrp3^tm1Hhf/Nlrp3⁺ Lyz2^tm1(cre)Ifo/Lyz2⁺	involves: 129/Sv * 129P2/OlaHsd * C57BL/6	MGI:3850045
cn6	Nlrp3^tm1Hhf/Nlrp3⁺ Tg(CAG-cre/Esr1*)5Amc/?	involves: 129/Sv * C57BL/6 * CBA	MGI:3850050
cn7	Nlrp3^tm1Hhf/Nlrp3⁺ Tg(Zp3-cre)3Mrt/?	involves: 129/Sv * FVB/N	MGI:3850044
cx8	Nlrp3^tm1Hhf/Nlrp3⁺ Tg(CAG-cre/Esr1*)5Amc/?	involves: 129/Sv * C57BL/6 * CBA	MGI:3850049

Allelic Composition	Nlrp3^tm1Flv/Nlrp3^tm1Hhf Lyz2^tm1(cre)Ifo/Lyz2⁺
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lyz2^tm1(cre)Ifo mutation (14 available); any Lyz2 mutation (40 available)
Nlrp3^tm1Flv mutation (1 available); any Nlrp3 mutation (64 available)
Nlrp3^tm1Hhf mutation (1 available); any Nlrp3 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:150054 )

• mice die between 2 and 14 days after birth probably due to multisystem organ failure secondary to inflammation and necrosis

• genotype demonstrates inflammatory disease of the conditional allele does not require presence of wild-type allele

immune system

increased inflammatory response ( J:150054 )

• mice have pronounced leukocytic infiltrates in skin, liver, spleen, joint, sinus, conjunctiva, bone marrow, and tongue that are mainly neutrophilic

Allelic Composition	Il1r1^tm1Roml/Il1r1^tm1Roml Nlrp3^tm1Hhf/Nlrp3⁺ Lyz2^tm1(cre)Ifo/Lyz2⁺
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il1r1^tm1Roml mutation (2 available); any Il1r1 mutation (39 available)
Lyz2^tm1(cre)Ifo mutation (14 available); any Lyz2 mutation (40 available)
Nlrp3^tm1Hhf mutation (1 available); any Nlrp3 mutation (64 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:150054 )

N	• postnatal lethality does not occur to mice on a IL-1 receptor null background

immune system

skin inflammation ( J:150054 )

• variable skin inflammation is observed

integument

skin inflammation ( J:150054 )

• variable skin inflammation is observed

Allelic Composition	Nlrp3^tm1Hhf/Nlrp3⁺ Pycard^tm1Flv/Pycard^tm1Flv Lyz2^tm1(cre)Ifo/Lyz2⁺
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lyz2^tm1(cre)Ifo mutation (14 available); any Lyz2 mutation (40 available)
Nlrp3^tm1Hhf mutation (1 available); any Nlrp3 mutation (64 available)
Pycard^tm1Flv mutation (0 available); any Pycard mutation (15 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:150054 )

• mice appear phenotypically normal and do not develop the inflammatory disease that conditional heterozygotes develop on an intact Pycard background

Allelic Composition	Nlrp3^tm1Hhf/Nlrp3⁺ Rag1^tm1Mom/Rag1^tm1Mom Lyz2^tm1(cre)Ifo/Lyz2⁺
Genetic Background	involves: 129/Sv * 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lyz2^tm1(cre)Ifo mutation (14 available); any Lyz2 mutation (40 available)
Nlrp3^tm1Hhf mutation (1 available); any Nlrp3 mutation (64 available)
Rag1^tm1Mom mutation (49 available); any Rag1 mutation (123 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:150054 )

• mice die between 2 and 14 days after birth probably due to multisystem organ failure secondary to inflammation and necrosis

• 70% of mice die between 7-14 days

• absence of B and T cells demonstrates disease is not caused by the adaptive immune system

growth/size/body

slow postnatal weight gain ( J:150054 )

• mutant pups gained weight slowly, and then lost weight before dying

immune system

absent B cells ( J:150054 )

absent T cells ( J:150054 )

hematopoietic system

absent B cells ( J:150054 )

absent T cells ( J:150054 )

integument

reddish skin ( J:150054 )

• 1-2 day old mice have skin abscesses that develop into erythema and scaly skin by day 4

scaly skin ( J:150054 )

• 1-2 day old mice have skin abscesses that develop into erythema and scaly skin by day 4

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:150054 )

• mice die between 2 and 14 days after birth probably due to multisystem organ failure secondary to inflammation and necrosis

• 70% of mice die between 7-14 days

growth/size/body

slow postnatal weight gain ( J:150054 )

• mutant pups gained weight slowly, and then lost weight before dying

digestive/alimentary system

abnormal intestine morphology ( J:150054 )

• substantial necrosis occurs in the gut that is not associated with inflammation

renal/urinary system

abnormal kidney morphology ( J:150054 )

• substantial necrosis occurs in the kidney that is not associated with inflammation

immune system

increased leukocyte cell number ( J:150054 )

• white blood cell count is mildly elevated

increased neutrophil cell number ( J:150054 )

• pronounced neutrophilia is evident in these mice

abnormal circulating cytokine level ( J:150054 )

• GCSF is elevated in the sera of 6-8 day old mice

abnormal circulating chemokine level ( J:150054 )

• CXCL1 levels are elevated in the sera of mice 6-8 days old

increased circulating interleukin-1 beta level ( J:150054 )

• IL-1beta levels in the sera of 6-8 day old mice are elevated 3-fold

• levels of this cytokine are also elevated in the dermis and epidermis, which may contribute to the skin pathology

increased circulating interleukin-18 level ( J:150054 )

• IL-18 levels are elevated in the sera of 6-8 day old mice

increased circulating interleukin-6 level ( J:150054 )

• IL-6 levels are elevated in the sera of 6-8 day old mice

• levels of this cytokine are also elevated in the dermis and epidermis, which may contribute to the skin pathology

increased circulating tumor necrosis factor level ( J:150054 )

• TNF levels in the sera of 6-8 day old mice are elevated

increased inflammatory response ( J:150054 )

• mice have pronounced leukocytic infiltrates in skin, liver, spleen, joint, sinus, conjunctiva, bone marrow, and tongue that are mainly neutrophilic

homeostasis/metabolism

abnormal circulating cytokine level ( J:150054 )

• GCSF is elevated in the sera of 6-8 day old mice

abnormal circulating chemokine level ( J:150054 )

• CXCL1 levels are elevated in the sera of mice 6-8 days old

increased circulating interleukin-1 beta level ( J:150054 )

• IL-1beta levels in the sera of 6-8 day old mice are elevated 3-fold

• levels of this cytokine are also elevated in the dermis and epidermis, which may contribute to the skin pathology

increased circulating interleukin-18 level ( J:150054 )

• IL-18 levels are elevated in the sera of 6-8 day old mice

increased circulating interleukin-6 level ( J:150054 )

• IL-6 levels are elevated in the sera of 6-8 day old mice

• levels of this cytokine are also elevated in the dermis and epidermis, which may contribute to the skin pathology

increased circulating tumor necrosis factor level ( J:150054 )

• TNF levels in the sera of 6-8 day old mice are elevated

hematopoietic system

thrombocytosis ( J:150054 )

• thrombocytosis is evident in these mice

increased leukocyte cell number ( J:150054 )

• white blood cell count is mildly elevated

increased neutrophil cell number ( J:150054 )

• pronounced neutrophilia is evident in these mice

integument

hairless ( J:150054 )

• hair growth does not occur in these mice

reddish skin ( J:150054 )

• 1-2 day old mice have skin abscesses that develop into erythema and scaly skin by day 4

scaly skin ( J:150054 )

• 1-2 day old mice have skin abscesses that develop into erythema and scaly skin by day 4

Allelic Composition	Nlrp3^tm1Hhf/Nlrp3⁺ Tg(CAG-cre/Esr1*)5Amc/?
Genetic Background	involves: 129/Sv * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nlrp3^tm1Hhf mutation (1 available); any Nlrp3 mutation (64 available)
Tg(CAG-cre/Esr1*)5Amc mutation (10 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal macrophage physiology ( J:150054 )

• peritoneal macrophages are able to secrete IL-1beta in the absence of ATP when activated in vitro

abnormal dendritic cell physiology ( J:150054 )

• bone marrow derived dendritic cells hypersecrete the mature form of IL-1beta in response to activation

• the maximal response is 300-fold greater than controls

• DCs are able to secrete IL1-beta without the presence of exogenous ATP

abnormal dendritic cell antigen presentation ( J:150054 )

• mutant DCs cells have an enhanced ability to stimulate T cells to differentiate into a Th17 subtypes when presenting cognate antigen

• mutant DCs also enhance Th1 and Th2 differentation when co-cultured with T cells in the presence of polarizing cytokines

hematopoietic system

abnormal macrophage physiology ( J:150054 )

• peritoneal macrophages are able to secrete IL-1beta in the absence of ATP when activated in vitro

Allelic Composition	Nlrp3^tm1Hhf/Nlrp3⁺ Tg(Zp3-cre)3Mrt/?
Genetic Background	involves: 129/Sv * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nlrp3^tm1Hhf mutation (1 available); any Nlrp3 mutation (64 available)
Tg(Zp3-cre)3Mrt mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:150054 )

• mice die between 2 and 14 days after birth probably due to multisystem organ failure secondary to inflammation and necrosis

growth/size/body

slow postnatal weight gain ( J:150054 )

• mutant pups gained weight slowly, and then lost weight before dying

immune system

increased leukocyte cell number ( J:150054 )

• white blood cell count is mildly elevated

increased neutrophil cell number ( J:150054 )

• pronounced neutrophilia is evident in these mice

increased inflammatory response ( J:150054 )

• mice have pronounced leukocytic infiltrates in skin, liver, spleen, joint, sinus, conjunctiva, bone marrow, and tongue that are mainly neutrophilic

digestive/alimentary system

abnormal intestine morphology ( J:150054 )

• substantial necrosis occurs in the gut that is not associated with inflammation

renal/urinary system

abnormal kidney morphology ( J:150054 )

• substantial necrosis occurs in the kidney that is not associated with inflammation

hematopoietic system

thrombocytosis ( J:150054 )

• thrombocytosis is evident in these mice

increased leukocyte cell number ( J:150054 )

• white blood cell count is mildly elevated

increased neutrophil cell number ( J:150054 )

• pronounced neutrophilia is evident in these mice

integument

hairless ( J:150054 )

• hair growth does not occur in these mice

reddish skin ( J:150054 )

• 1-2 day old mice have skin abscesses that develop into erythema and scaly skin by day 4

scaly skin ( J:150054 )

• 1-2 day old mice have skin abscesses that develop into erythema and scaly skin by day 4

Allelic Composition	Nlrp3^tm1Hhf/Nlrp3⁺ Tg(CAG-cre/Esr1*)5Amc/?
Genetic Background	involves: 129/Sv * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nlrp3^tm1Hhf mutation (1 available); any Nlrp3 mutation (64 available)
Tg(CAG-cre/Esr1*)5Amc mutation (10 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

slow postnatal weight gain ( J:150054 )

• some older mice have slower weight gain

immune system

skin inflammation ( J:150054 )

• some older mice develop skin inflammation suggesting leaky expression of the cre recombinase

integument

skin inflammation ( J:150054 )

• some older mice develop skin inflammation suggesting leaky expression of the cre recombinase

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