Analysis Tools
mortality/aging
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• offspring of treated female mice die shortly after birth
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growth/size/body
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• by six weeks of age, animals display a dwarf phenotype; growth stops around 6 weeks
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• pups from transgenic females treated with doxycycline during gestation are not distinguishable from controls animals during the initial 10 days post-natal; by 14 days, animals are visibly smaller than controls
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• at birth, weight of animals treated with doxycycline is comparable to controls but lags behind during postnatal period; when growth stops, mice have weights about half to two thirds that of control
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endocrine/exocrine glands
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• at 6 weeks, gonadotropin staining is slightly reduced compared to wild-type adults or age-matched controls
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• prolactin-staining cells show marked reduction in number
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• nearly complete absence of growth hormone-staining cells is observed at 6 weeks
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• anterior lobe in doxycycline-treated mice (treatment from conception or from E14) are significantly smaller than wild-type at 6 weeks
• however, posterior and intermediate lobes are normal
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reproductive system
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• dwarf females can carry pregnancies to birth, but offspring die shortly after birth, suggesting that the dams have impaired lactation
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nervous system
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• at 6 weeks, gonadotropin staining is slightly reduced compared to wild-type adults or age-matched controls
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• prolactin-staining cells show marked reduction in number
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• nearly complete absence of growth hormone-staining cells is observed at 6 weeks
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• anterior lobe in doxycycline-treated mice (treatment from conception or from E14) are significantly smaller than wild-type at 6 weeks
• however, posterior and intermediate lobes are normal
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vision/eye
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• eye opening of mutants is delayed by 2-3 days relative to wild-type or single positive controls
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