All Phenotypes Rheb<tm1Pfw> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Rheb^tm1Pfw
targeted mutation 1, Paul Worley
MGI:3850507

Summary

4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Amh-cre)8815Reb/0	involves: 129S1/SvImJ * FVB/N	MGI:6460092
cn2	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Cd4-cre)1Cwi/0	involves: C57BL/6 * DBA/2	MGI:3850517
cn3	Gt(ROSA)26Sor^{tm1(CAG-Rheb*)Pfw}/Gt(ROSA)26Sor⁺ Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL	MGI:4939481
cn4	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL	MGI:4939480

Allelic Composition	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Amh-cre)8815Reb/0
Genetic Background	involves: 129S1/SvImJ * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rheb^tm1Pfw mutation (0 available); any Rheb mutation (26 available)
Tg(Amh-cre)8815Reb mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:268375 )

N	• adult males are fertile, have many normal sperm present in the epididymis, and show normal distribution of vimentin filaments in Sertoli cells (SCs) at 2 months of age, suggesting normal SC cytoskeletal organization

oligozoospermia ( J:268375 )

• 70% reduction in sperm count at 2 months of age

abnormal seminiferous tubule morphology ( J:268375 )

• adult mice display only mild morphological alterations in the seminiferous tubules

decreased testis weight ( J:268375 )

• 60% reduction in testis weight at 2 months of age

decreased epididymis weight ( J:268375 )

• 54% reduction in epididymis weight at 2 months of age

cellular

oligozoospermia ( J:268375 )

• 70% reduction in sperm count at 2 months of age

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:268375 )

• adult mice display only mild morphological alterations in the seminiferous tubules

decreased testis weight ( J:268375 )

• 60% reduction in testis weight at 2 months of age

Allelic Composition	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Cd4-cre)1Cwi/0
Genetic Background	involves: C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rheb^tm1Pfw mutation (0 available); any Rheb mutation (26 available)
Tg(Cd4-cre)1Cwi mutation (10 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:150108 )

N	• despite inhibition of TORC1 activity, T cells exhibit normal regulatory T cell differentiation

Allelic Composition	Gt(ROSA)26Sor^{tm1(CAG-Rheb*)Pfw}/Gt(ROSA)26Sor⁺ Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Nes-cre)1Kln/0
Genetic Background	involves: C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm1(CAG-Rheb*)Pfw} mutation (0 available); any Gt(ROSA)26Sor mutation (1006 available)
Rheb^tm1Pfw mutation (0 available); any Rheb mutation (26 available)
Tg(Nes-cre)1Kln mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

nervous system phenotype ( J:168571 )

N	• brain size, myelination, and oligodendrocyte numbers are similar to controls unlike in mutant mice without the knock-in allele in Gt(ROSA)26Sor

Allelic Composition	Rheb^tm1Pfw/Rheb^tm1Pfw Tg(Nes-cre)1Kln/0
Genetic Background	involves: C57BL/6 * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rheb^tm1Pfw mutation (0 available); any Rheb mutation (26 available)
Tg(Nes-cre)1Kln mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:168571 )

• most die at 5-6 weeks of age

nervous system

abnormal brain development ( J:168571 )

• increase in the number of oligodendrocyte precursors in the cortex and hippocampus at 3 weeks of age

decreased brain weight ( J:168571 )

• a decrease in brain weight is detected at P5-P9 but not at P1

• by 2-3 weeks of age brain weight is about 50% that of controls

thin cerebral cortex ( J:168571 )

• at 4 weeks of age cortical thickness is about 65% that of controls

• however, no reduction in neuronal counts is detected at P1, P5 or P28

decreased oligodendrocyte number ( J:168571 )

• the number of mature oligodendrocytes is reduced by 60-70% in multiple regions of the brain, including the corpus callosum, cortex, and hippocampus

• the total number of oligodendrocytes (OLIG2+ cells) is reduced especially in the corpus callosum and to a lesser extent in the cortex and hippocampus

abnormal myelin sheath morphology ( J:168571 )

• widespread loss of fine myelin binding protein reactive fibers in all cortical layers and in neuropil regions of the hippocampus

• decrease in the number of meylinated axons in the corpus callosum

demyelination ( J:168571 )

• prominent at 4 weeks of age

• reduced levels of myelin proteins are detected throughout the brain

• decrease in myelination in the dense white matter tracts

growth/size/body

postnatal growth retardation ( J:168571 )

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